Methods to Assess Vascular Permeability During Angiogenic Processes

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Abstract

Angiogenesis is a multi-faceted process that is, in part, reliant on dynamic regulation of the endothelium that lines the bodies vasculature. Here we have detailed angiogenic factors VEGF, angiopoietin 1 and 2, sphingosine 1-Phosphate (S1P), and hepatocyte growth factor, all of which act, in part, via alterations of endothelial barrier function. As these alterations in endothelial integrity provide insight into angiogenic responses, we further specify detailed protocols that may be used in its study. These include Transwell Albumin clearance, Evans Blue Albumin Permeability assays, and Trans-endothelial Electrical Resistance.

Keywords

Vascular Endothelial Growth Factor Hepatocyte Growth Factor Evans Blue Cortical Actin Stalk Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  1. 1.Department of PharmacologyThe University of Illinois at ChicagoChicagoUSA
  2. 2.Department of Medicine, Institute for Personalized Respiratory MedicineThe University of Illinois at ChicagoChicagoUSA
  3. 3.Earl Bane Professor of Medicine, Pharmacology and BioengineeringThe University of Illinois at ChicagoChicagoUSA

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