Simultaneous Determination of Sulfur Metabolites in Plant Tissue by LC-ESI-MS/MS

Conference paper
Part of the Proceedings of the International Plant Sulfur Workshop book series (PIPSW, volume 1)

Abstract

An analytical method was developed for the accurate quantification of the contents of sulfur metabolites of plant tissues based on liquid chromatography – electrospray ionization – tandem mass spectrometry (LC-ESI-MS/MS). 34S-Labeled sulfur metabolites were biosynthesized in Arabidopsis thaliana from 34S-SO 4 2− and used as internal standards for the analysis and quantification of these sulfur-containing metabolites in A. thaliana and O. banksiaefolia fern spores.

Keywords

Flower Stem Fern Spore GSSG Content Photon Fluence Rate Sulfur Metabolite 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors acknowledge Dr. Ding-Tzai Li for fruitful discussions and Mass Solution Technology Co., Ltd for excellent technical support.

References

  1. Annesley TM (2003) Ion suppression in mass spectrometry. Clin Chem 49:1041–1044PubMedCrossRefGoogle Scholar
  2. Bajad S, Shulaev V (2007) Highly-parallel metabolomics approaches using LC-MS2 for pharmaceutical and environmental analysis. Trends Anal Chem 26:625–636CrossRefGoogle Scholar
  3. Bennett BD, Yuan J, Kimball EH, Rabinowitz JD (2008) Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach. Nat Protoc 3:1299–1311PubMedCrossRefGoogle Scholar
  4. Harwood DT, Kettle AJ, Brennan S, Winterbourn CC (2009) Simultaneous determination of reduced glutathione, glutathione disulphide and glutathione sulphonamide in cells and physiological fluids by isotope dilution liquid chromatography-tandem mass spectrometry. J Chromatogr B 877:3393–3399CrossRefGoogle Scholar
  5. Hawkesford MJ, De Kok LJ (2006) Managing sulphur metabolism in plants. Plant Cell Environ 29:382–395PubMedCrossRefGoogle Scholar
  6. Jacob C (2006) A scent of therapy: pharmacological implications of natural products containing redox-active sulfur atoms. Nat Prod Rep 23:851–863PubMedCrossRefGoogle Scholar
  7. Koralewska A, Buchner P, Stuiver CEE, Posthumus FS, Kopriva S, Hawkesford MJ, De Kok LJ (2009) Expression and activity of sulfate transporters and APS reductase in curly kale in response to sulfate deprivation and re-supply. J Plant Physiol 166:168–179PubMedCrossRefGoogle Scholar
  8. Lloyd RM, Klekowski EJ (1970) Spore germination and viability in pteridophyta: evolutionary significance of chlorophyllous spores. Biotropica 2:129–137CrossRefGoogle Scholar
  9. Lu W, Bennett BD, Rabinowitz JD (2008) Analytical strategies for LC-MS-based targeted metabolomics. J Chromatogr B 871:236–242CrossRefGoogle Scholar
  10. Mougous JD, Leavell MD, Senaratne RH, Leigh CD, Williams SJ, Riley LW, Leary JA, Bertozzi CR (2002) Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach. Proc Natl Acad Sci USA 99:17037–17042PubMedCrossRefGoogle Scholar
  11. Patzelt H, Keβler B, Oschkinat H, Oesterhelt D (1999) The entire metabolite spectrum of the green alga Scenedesmus obliquus in isotope-labelled form. Phytochemistry 50:215–217CrossRefGoogle Scholar
  12. Remane D, Wissenbach DK, Meyer MR, Maurer HH (2010) Systematic investigation of ion suppression and enhancement effects of fourteen stable-isotope-labeled internal standards by their native analogues using atmospheric-pressure chemical ionization and electrosprayionization and the relevance for multi-analyte liquid chromatographic/mass spectrometric procedures. Rapid Commun Mass Spectrom 24:859–867PubMedCrossRefGoogle Scholar
  13. Shahbaz M, Tseng MH, Stuiver CEE, Koralewska A, Posthumus FS, Venema JH, Parmar S, Schat H, Hawkesford MJ, De Kok LJ (2010) Copper exposure interferes with the regulation of the uptake, distribution and metabolism of sulfate in Chinese cabbage. J Plant Physiol 167:438–446PubMedCrossRefGoogle Scholar
  14. Wu L, Mashego MR, van Dam JC, Proell AM, Vinke JL, Ras C, van Winden WA, van Gulik WM, Heijnen JJ (2005) Quantitative analysis of the microbial metabolome by isotope dilution mass spectrometry using uniformly 13C-labeled cell extracts as internal standards. Anal Biochem 336:164–171PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Mei-Hwei Tseng
    • 1
  • Luit J. De Kok
    • 2
  • Yao-Moan Huang
    • 3
  1. 1.Department of Applied Physics and ChemistryTaipei Municipal University of EducationTaipeiTaiwan
  2. 2.Laboratory of Plant PhysiologyUniversity of GroningenGroningenThe Netherlands
  3. 3.Division of SilvicultureTaiwan Forestry Research InstituteTaipeiTaiwan

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