Bone Reconstruction Utilizing Mesenchymal Stem Cell Sheets for Cell Delivery
Large bone defects often arise from traumatic injury. Mesenchymal stem cells (MSCs) hold great potential for bone regeneration. However to date, MSCs have not yet been incorporated into structural bone allografts in clinical practice. MSCs possess high proliferative capacity and the potential to differentiate into at least three mesodermal lineages – bone, cartilage and fat. The high proliferative capacity of bMSCs enables a more rapid formation of cell sheets compared to terminally differentiated cell types. The multi-lineage differentiation potential of MSCs broadens the application of the cell sheet technique, providing a wider scope of application for connective tissue engineering. In particular, assembly of MSC sheets and large allografts provides a convenient and practical tissue engineering platform for clinical regeneration of large musculoskeletal defects. This is anticipated to be a major future direction for enhancing allograft healing and repair via tissue engineering and stem cell engraftment.
KeywordsBone Graft Bone Tissue Engineering Cell Sheet Demineralized Bone Matrix Large Bone Defect
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