Aging Research in Yeast pp 101-121
Chronological Aging in Saccharomyces cerevisiae
The two paradigms to study aging in Saccharomyces cerevisiae are the chronological life span (CLS) and the replicative life span (RLS). The chronological life span is a measure of the mean and maximum survival time of non-dividing yeast populations while the replicative life span is based on the mean and maximum number of daughter cells generated by an individual mother cell before cell division stops irreversibly. Here we review the principal discoveries associated with yeast chronological aging and how they are contributing to the understanding of the aging process and of the molecular mechanisms that may lead to healthy aging in mammals. We will focus on the mechanisms of life span regulation by the Tor/Sch9 and the Ras/adenylatecyclase/PKA pathways with particular emphasis on those implicating age-dependent oxidativestress and DNA damage/repair.
KeywordsChronological aging TOR (target of rapamycin) RAS Adaptive regrowth Caloric restriction
- Fabrizio P et al (2010), Genome-wide screen in Saccharomyces cerevisiae identifies vacuolar protein sorting, autophagy, biosynthetic, and tRNA methylation genes involved in life span regulation. PLoS Genet 6(8):1227–1228Google Scholar
- Longo V (1997) The chronological life span of Saccharomyces cerevisiae. Studies of superoxide dismutase, Ras and Bcl-2. Thesis, University of California, Los Angeles, CAGoogle Scholar
- Schriner SE, Linford NJ, Martin GM, Treuting P, Ogburn CE et al (2005) Extension of murine lifespan by overexpression of catalase targeted to mitochondria. Science 308(5730):1909–1911Google Scholar