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Genome-Wide Analysis of Yeast Aging

  • George L. Sutphin
  • Brady A. Olsen
  • Brian K. Kennedy
  • Matt Kaeberlein
Part of the Subcellular Biochemistry book series (SCBI, volume 57)

Abstract

In the past several decades the budding yeast Saccharomyces cerevisiae has emerged as a prominent model for aging research. The creation of a single-gene deletion collection covering the majority of open reading frames in the yeast genome and advances in genomic technologies have opened yeast research to genome-scale screens for a variety of phenotypes. A number of screens have been performed looking for genes that modify secondary age-associated phenotypes such as stress resistance or growth rate. More recently, moderate-throughput methods for measuring replicative life span and high-throughput methods for measuring chronological life span have allowed for the first unbiased screens aimed at directly identifying genes involved in determining yeast longevity. In this chapter we discuss large-scale life span studies performed in yeast and their implications for research related to the basic biology of aging.

Keywords

Acetic acid Apoptosis Asymmetric segregation Chronological life span Counter flow centrifugation elutriation (CCE) Dietary restriction (DR) Genome-wide Genomics High-throughput Loss of heterozygosity (LOH) Metabolomics Microarrays Mitochondria Mitochondrial back-signaling Mother Enrichment Program (MEP) Oxidative damage Proteomics Replicative life span Retrograde response Ribosomal DNA (rDNA) Sirtuins Target of rapamycin (TOR) signaling Translation Worms Yeast Outgrowth Data Analysis (YODA) 

Abbreviations and Accronyms

CCE

counter flow centrifugation elutriation

DR

dietary restriction

ERC

extrachromosomal rDNA circles

FNR

false negative rate

FPR

false positive rate

LL

long-lived

LLM

long-lived mutant

MEP

mother enrichment program

NLL

not long-lived

NSE

no significant extension

OD

optical density

ORF

open reading frame

PKA

protein kinase A

ROS

reactive oxygen species

SL

short-lived

SIR

silent information regulator

TOR

target of rapamycin

uORF

upstream open reading frame

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • George L. Sutphin
    • 3
  • Brady A. Olsen
    • 1
  • Brian K. Kennedy
    • 2
  • Matt Kaeberlein
    • 1
  1. 1.Department of PathologyUniversity of WashingtonSeattleUSA
  2. 2.Buck InstituteNovatoUSA
  3. 3.Department of Pathology and the Molecular and Cellular Biology ProgramUniversity of WashingtonSeattleUSA

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