Superresolution Optical Fluctuation Imaging (SOFI)

  • Thomas Dertinger
  • Ryan Colyer
  • Robert Vogel
  • Mike Heilemann
  • Markus Sauer
  • Jörg Enderlein
  • Shimon Weiss
Chapter

Abstract

Superresolution microscopy has shifted the limits for fluorescence microscopy in cell ­biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed “superresolution optical fluctuation ­imaging” (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.

Keywords

Superresolution Statistical analysis Correlation function 

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Thomas Dertinger
    • 1
  • Ryan Colyer
    • 1
  • Robert Vogel
    • 1
  • Mike Heilemann
    • 2
  • Markus Sauer
    • 3
  • Jörg Enderlein
    • 4
  • Shimon Weiss
    • 1
    • 5
    • 6
  1. 1.Department of Chemistry and BiochemistryUniversity of California Los AngelesLos AngelesUSA
  2. 2.Department of Physics, Applied Laser PhysicsBielefeld UniversityBielefeldGermany
  3. 3.Department of Biotechnology and BiophysicsJulius-Maximilians-Universität WürzburgWürzburgGermany
  4. 4.III Institute of PhysicsGeorg August UniversityGöttingenGermany
  5. 5.Department of PhysiologyUniversity of California Los Angeles, UCLALos AngelesUSA
  6. 6.California NanoSystems Institute, University of California Los Angeles, UCLALos AngelesUSA

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