Transient Expression Using Agroinfiltration and Its Applications in Molecular Farming

Chapter

Abstract

Transient expression via agroinfiltration and/or viral vectors has quickly emerged as the preferred expression system for plant-made recombinant proteins. Transient expression can serve as a valuable research tool for finding optimal expression parameters before tedious and time-consuming production of stable transgenic plants or it can be scaled up to commercial production scale with vacuum infiltration. This technology is poised to compete with conventional production systems, large-scale production facilities are currently available and others are in the process of development. This chapter will introduce background and rationale in development of transient expression systems in plants and summarize the latest developments and examples in this area.

Keywords

Human Immunodeficiency Virus Recombinant Protein Human Papilloma Virus Vacuum Infiltration Transient Expression System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgement

This work was supported by a grant from an A-base grant from Agriculture and Agri-Food Canada and the Agricultural Bioproducts Innovation Program.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Southern Crop Protection and Food Research CentreAgriculture and Agri-Food CanadaLondonCanada
  2. 2.VTT Biotechnology, Technical Research Centre of FinlandEspooFinland

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