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Photosynthesis pp 113-126 | Cite as

Assembly of Light Harvesting Pigment-Protein Complexes in Photosynthetic Eukaryotes

  • Tomas Morosinotto
  • Roberto BassiEmail author
Chapter
Part of the Advances in Photosynthesis and Respiration book series (AIPH, volume 34)

Summary

In photosynthetic eukaryotes, the antenna system includes members of a protein family of Light-harvesting complexes encoded by the Lhc genes. These proteins bind 8–14 chlorophylls (Chls) and 2–4 carotenoid molecules per 22–28 kDa polypeptide; further, the pigments are needed for the assembly of monomeric proteins. Some members form dimers (LHCI) or trimers (LHCII). Chl a is needed for the assembly of all the Lhc proteins, while two members, Lhca1 and Lhca3, can refold in vitro without Chl b. Among carotenoids, lutein is bound to site L1 in all the Lhc proteins, whose occupancy is essential for protein assembly. Violaxanthin and zeaxanthin can also drive protein folding, although with a lower efficiency with respect to lutein. Current knowledge on the assembly mechanisms is also reviewed in this chapter: in vitro experiments have shown how Lhc folding is triggered by the binding of Chl a and of lutein; in contrast Chl b is only bound in a second phase and functions to stabilize the pigment-protein complexes. Together with the reaction centers, antenna complexes are organized as supercomplexes in the thylakoid membranes. The structural organization of the antenna of Photosystem I (PS I) is quite different from that of Photosystem II (PS II): the PS II antenna system is flexible and its size is modulated according to environmental conditions, while in PS I antenna protein content is maintained constant with respect to the reaction center.

Keywords

Antenna System High Light Condition Antenna Size Antenna Complex Antenna Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations:

Chl –

Chlorophyll;

Lhca (b) –

Light-harvesting complex of Photosystem I (II);

LHCI (II) –

Antenna complex of Photosystem I (II);

PQ –

Plastoquinone;

PS I (II) –

Photosystem I (II)

Notes

Acknowledgements

Authors thank all present and past co-workers for useful discussions. R.B. acknowledges funding from FIRB RBLA0345SF002 (Solanacee) and RBIP06CPBR_006 (Parallelomics). R.B. would also like to thank the von Humbolt association for support.

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© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.Dipartimento di BiologiaUniversità di PadovaPadovaItaly
  2. 2.Dipartimento di BiotecnologieUniversità di VeronaVeronaItaly
  3. 3.ICG-3: PhytosphäreJülichGermany

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