Multi-parameter Process Optimization Using the SimCell™ System

  • A. Peter Russo
  • Brian Benoit
  • Christian Wood
  • David Jan
  • Sadettin S. Ozturk
Conference paper
Part of the ESACT Proceedings book series (ESACT, volume 5)

Abstract

Bioprocess development requires the use of scale-down models for clone selection, media optimization, process development and characterization. Different models are commonly used at various stages: well plates and shake flasks for clone selection, shake flasks and bench-top bioreactors for media optimization and process development, bench-scale and larger bioreactors for process characterization. The choice of model is based on a combination of throughput, economics and similarity to the final manufacturing scale. The SimCell System provides a high-throughput micro-bioreactor platform which can perform these various tasks under bioreactor relevant conditions (Legmann et al. 2009). By using such a system, clone, media and process optimization can be conducted in parallel earlier in the development process, resulting in significant time savings, as well as the ability to identify significant interactions between factors.

Keywords

Shake Flask Central Composite Design Chinese Hamster Ovary Cell Line Product Titer Total Cell Density 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Reference

  1. Legmann R, Schreyer HB, Combs RG, McCormick EL, Russo AP, Rodgers ST. 2009. A predictive high-throughput scale-down model of monoclonal antibody production in CHO cells. Biotechnol Bioeng. 104(6):1107–20.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • A. Peter Russo
    • 1
    • 2
  • Brian Benoit
    • 1
    • 2
  • Christian Wood
    • 3
  • David Jan
    • 3
  • Sadettin S. Ozturk
    • 4
    • 5
  1. 1.Seahorse BioscienceNorth BillericaUSA
  2. 2.BioProcessors CorporationWoburnUSA
  3. 3.CentocorMalvernUSA
  4. 4.Centocor R&DMalvernUSA
  5. 5.Johnson & Johnson & JohnsonSkillmanUSA

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