Multi-parameter Process Optimization Using the SimCell™ System
Bioprocess development requires the use of scale-down models for clone selection, media optimization, process development and characterization. Different models are commonly used at various stages: well plates and shake flasks for clone selection, shake flasks and bench-top bioreactors for media optimization and process development, bench-scale and larger bioreactors for process characterization. The choice of model is based on a combination of throughput, economics and similarity to the final manufacturing scale. The SimCell System provides a high-throughput micro-bioreactor platform which can perform these various tasks under bioreactor relevant conditions (Legmann et al. 2009). By using such a system, clone, media and process optimization can be conducted in parallel earlier in the development process, resulting in significant time savings, as well as the ability to identify significant interactions between factors.