Development of a Biphasic Culture Process for Recombinant Protein Production in Human CAP Cells

  • Ruth Essers
  • Helmut Kewes
  • Gudrun Schiedner
Conference paper
Part of the ESACT Proceedings book series (ESACT, volume 5)


In order to evaluate physical parameters for batch cultivation of stable human CAP cell lines, a biphasic batch process with pH-shift was established. Importantly, the biphasic process resulted in a significant increase of volumetric productivity of a highly complex and fully glycosylated protein. To achieve high product yields of recombinant protein in stable cell lines, increased growth rate and high productivity are needed. Low levels of inhibitory metabolic products and sufficient substrate supply are known to lead to extended process time, increased volumetric productivity and optimized product quality.


Stable Cell Line Viable Cell Density Amniotic Fluid Cell Final Product Concentration Lactate Production Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  1. 1.CEVEC Pharmaceuticals GmbHKölnGermany
  2. 2.CEVEC Pharmaceuticals GmbHKölnGermany

Personalised recommendations