Fibrolase and Its Evolution to Clinical Trials: A Long and Winding Road

Chapter

Abstract

Fibrolase is the fibrinolytic proteinase isolated from Agkistrodon contortrix contortrix (southern copperhead snake) venom. The enzyme was purified by a three-step HPLC procedure and was shown to be homogeneous by standard criteria. The purified enzyme is inhibited by EDTA and other chelating agents and is a zinc metalloproteinase containing one mole of zinc per molecule. The enzyme is also rapidly inhibited by alpha2-macroglobulin (α2 M). Fibrolase is composed of 203 amino acids with a blocked amino-terminus due to cyclization of the terminal Gln residue. The enzyme is a direct-acting thrombolytic agent and does not rely on plasminogen for clot dissolution. Fibrolase rapidly cleaves the A(α)-chain of fibrinogen and the B(β)-chain at a slower rate; it has no activity on the γ-chain. The enzyme exhibits the same specificity with fibrin. Fibrolase was shown to have very effective thrombolytic activity in a reoccluding carotid arterial thrombosis model in the canine. A recombinant version of the enzyme was made in yeast by Amgen, Inc. (Thousand Oaks, CA, USA) and called alfimeprase. Alfimeprase is identical to fibrolase except for a two amino acid truncation at the amino-terminus and the insertion of a new amino-terminal amino acid in the truncated protein; these changes lead to a more stable enzyme for prolonged storage. Twenty years after it was first purified alfimeprase was taken into clinical trials by Nuvelo, Inc. (San Carlos, CA), which licensed the enzyme from Amgen. Alfimeprase was successful in Phase I and II clinical trials for peripheral arterial occlusion (PAO) and central venous access device (CVAD) occlusion. However, in Phase III trials alfimeprase did not meet the expected end points in either PAO or CVAD occlusion and in a Phase II stroke trial and Nuvelo dropped further development in 2008.

Keywords

Snake Venom Clot Lysis Fibrinolytic Enzyme Anisoylated Plasminogen Streptokinase Activator Complex Orphan Drug Status 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular BiologyUniversity of Southern California, Keck School of Medicine, Cancer Research Laboratory #106Los AngelesUSA
  2. 2.USC/Norris Comprehensive Cancer CenterUniversity of Southern California, Keck School of Medicine, Cancer Research Laboratory #106Los AngelesUSA

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