Entamoeba histolytica is morphologically identical to the non-pathogenic species Entamoeba dispar, and Entamoeba moshkovskii though genetic differences have confirmed the separation of these three as independent species [4, 13]. Due to this conserved morphology, stained smears of stool specimens are insufficient for differentiation of these species. While staining of fixed faecal smears can determine the presence of the Entamoeba histolytica/dispar/moshkovskii complex within a stool specimen, other techniques such as PCR or ELISA must be employed for differentiation. A number of PCR assays are available for detection and/or differentiation of Entamoeba species [3, 6, 11].
KeywordsTaqMan Assay Stool Specimen Entamoeba Histolytica Product Detection SYBR Green Assay
- 13.Som I, Azam A, Bhattacharya A et al (2000) Inter- and intra-strain variation in the 5.8S ribosomal RNA and internal transcribed spacer sequences of Entamoeba histolytica and comparison with Entamoeba dispar, Entamoeba moshkovskii and Entamoeba invadens. Int J Parasitol 30:723–728PubMedCrossRefGoogle Scholar