Universal Bacterial Identification by PCR and DNA Sequencing of 16S rRNA Gene
The assay is a single amplification PCR incorporating three separate reactions. The primers used are broad range primers which recognise conserved sequences within the 16S rRNA gene and amplify the intervening variable regions. The variable portions of the 16S rRNA gene provide unique signatures that can be analyzed to provide an identification of the bacteria species in the sample.
KeywordsCulture Isolate Sterile Site Commercial Service Provider Chromatography Fatty Acid Control Dilution Series
- 1.Clinical and Laboratory Standards Institute (2007) Interpretive criteria for microorganism identification by DNA target sequencing; proposed guideline. 27(22):MP18-PGoogle Scholar
- 3.Relman DA (1993) Universal bacterial 16S rRNA amplification and sequencing. In: Persing DH, Smith TF, Tenover FC, White TJ (eds) Diagnostic Molecular Biology, Principles and Applications. Mayo Foundation Rochester, MN, USA, pp 489–495Google Scholar