PCR for Clinical Microbiology

pp 209-214


Universal Bacterial Identification by PCR and DNA Sequencing of 16S rRNA Gene

  • Greg JamesAffiliated withCentre for Infectious Diseases and Microbiology Laboratory Services (CIDMLS), Institute for Clinical Pathology and Medical Research (ICPMR) Email author 

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The assay is a single amplification PCR incorporating three separate reactions. The primers used are broad range primers which recognise conserved sequences within the 16S rRNA gene and amplify the intervening variable regions. The variable portions of the 16S rRNA gene provide unique signatures that can be analyzed to provide an identification of the bacteria species in the sample.