Abstract
The assay is a single amplification PCR incorporating three separate reactions. The primers used are broad range primers which recognise conserved sequences within the 16S rRNA gene and amplify the intervening variable regions. The variable portions of the 16S rRNA gene provide unique signatures that can be analyzed to provide an identification of the bacteria species in the sample.
Keywords
- Culture Isolate
- Sterile Site
- Commercial Service Provider
- Chromatography Fatty Acid
- Control Dilution Series
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Clinical and Laboratory Standards Institute (2007) Interpretive criteria for microorganism identification by DNA target sequencing; proposed guideline. 27(22):MP18-P
Drancourt M, Raoult D (2005) Sequence based identification of new bacteria: a proposition for creation of an orphan bacterium repository. J Clin Microbiol 43:4311–4315
Relman DA (1993) Universal bacterial 16S rRNA amplification and sequencing. In: Persing DH, Smith TF, Tenover FC, White TJ (eds) Diagnostic Molecular Biology, Principles and Applications. Mayo Foundation Rochester, MN, USA, pp 489–495
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James, G. (2010). Universal Bacterial Identification by PCR and DNA Sequencing of 16S rRNA Gene. In: Schuller, M., Sloots, T., James, G., Halliday, C., Carter, I. (eds) PCR for Clinical Microbiology. Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9039-3_28
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DOI: https://doi.org/10.1007/978-90-481-9039-3_28
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