Universal Bacterial Identification by PCR and DNA Sequencing of 16S rRNA Gene

  • Greg JamesEmail author


The assay is a single amplification PCR incorporating three separate reactions. The primers used are broad range primers which recognise conserved sequences within the 16S rRNA gene and amplify the intervening variable regions. The variable portions of the 16S rRNA gene provide unique signatures that can be analyzed to provide an identification of the bacteria species in the sample.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Centre for Infectious Diseases and Microbiology Laboratory Services (CIDMLS)Institute for Clinical Pathology and Medical Research (ICPMR)WestmeadAustralia

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