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Development of Provisional Extracellular Matrix on Biomaterials Interface: Lessons from In Vitro Cell Culture

  • George AltankovEmail author
  • Thomas Groth
  • Elisabeth Engel
  • Jonas Gustavsson
  • Marta Pegueroles
  • Conrado Aparicio
  • Francesc J. Gil
  • Maria-Pau Ginebra
  • Josep A. Planell
Conference paper
Part of the NATO Science for Peace and Security Series A: Chemistry and Biology book series (NAPSA)

Abstract

The initial cellular events that take place at the biomaterials interface mimic to a certain extent the natural interaction of cells with the extracellular matrix (ECM). The cells adhering to the adsorbed soluble matrix proteins, such as fibronectin (FN) and fibrinogen (FNG) tend to re-arrange them in fibril-like pattern. Using model surfaces we have demonstrated that this cellular activity is abundantly dependent on the surface properties of materials, such as wettability, surface chemistry, charge and topography. This raises the possibility that tissue compatibility of materials is connected with the allowance of cells to remodel substratum associated proteins presumably to form provisional ECM. We have further shown that antibodies which bind β1 and αv integrins (subunits of the FN and FNG receptors respectively) may induce their linear rearrangement on the dorsal surface of living cells – a phenomenon presumably related to the same early molecular events of fibrillar matrix assembly. Because the quantitative measurements revealed that this receptor dynamics is strongly altered on the low compatible (hydrophobic) substrata we hypothesized that in order to be biocompatible, materials need to adsorb matrix proteins loosely, i.e. in such a way that the cells can easily remove and organize them in matrix-like fibrils via coordinated functioning of integrins. More recent studies on the fate of FN on some real biomaterial surfaces, including different rough titanium (Ti) and hydroxyapatite (HA) cements and the surface of biosensors confirmed this point of view. They also show that quantitative measurements of adsorbed matrix proteins and their dynamic rearrangement at cell-material interface might provide insight to the biocompatibility of given material and even predict its tissue integration.

Keywords

Biomaterials interface Fibronectin matrix Reorganizattion Provisional ECM Cellular interaction 

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • George Altankov
    • 1
    • 2
    • 4
    Email author
  • Thomas Groth
    • 5
  • Elisabeth Engel
    • 3
  • Jonas Gustavsson
    • 3
  • Marta Pegueroles
    • 3
  • Conrado Aparicio
    • 3
  • Francesc J. Gil
    • 3
  • Maria-Pau Ginebra
    • 3
  • Josep A. Planell
    • 3
  1. 1.Institute of Biophysics Bulgarian Academy of SciencesSofiaBulgaria
  2. 2.ICREA (Institucio Catala para Recercia i Estudis Avancats)BarcelonaSpain
  3. 3.Institute for Bioengineering of CataluniaL’Hospitalet de Llobregat BarcelonaSpain
  4. 4.Department of PharmacyMartin-Luther University Halle-WittenbergHalle (Saale)Germany
  5. 5.Department of Material ScienceUniversitat Politècnica de CatalunyaBarcelonaSpain

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