Recycling of the Seaweed Wakame Through Degradation by Halotolerant Bacteria

  • Jing-Chun Tang
  • Hideji Taniguchi
  • Qixing Zhou
  • Shinichi Nagata
Chapter
First Online:
Part of the Cellular Origin, Life in Extreme Habitats and Astrobiology book series (COLE, volume 15)

Abstract

A review on the recycling of seaweed by halotolerant bacteria was conducted for the cleaning of the marine environment. Planting of seaweed is effective in dealing with organic pollutants, especially N and P. Thus, the composting through recycling of seaweeds is supposed to be suitable, although their salinity is awkward in the composting process. The activity of inoculated bacteria decreased with the increase of salinity in wakame composting with Bacillus sp. HR6. On the contrary, halotolerant bacterium AW4, isolated, showed better degradation of wakame during composting than others that were examined. Degradation of alginate, which is the central component in wakame, is a key point in the recycling of seaweed. Thus, alginate-degrading bacterium A7 was isolated from the compost, by which alginate was successfully decomposed to 14.3% after 72 h of composting. The decomposition product of wakame by A7 sufficiently enhanced the relative root length of Komatsuna to 263.2%, suggesting that the applications of A7 to produce special fertilizer using seaweeds might be a meaningful way for preserving the seashore environment.

Keywords

Compost Process Viable Cell Number Alginate Lyase Halotolerant Bacterium Relative Root Length 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

References

  1. Bernal, M.P., Paredes, C., Sanchez-Monedero, M.A. and Cegarra, J. (1998) Maturity and stability parameters of composts prepared with a wide range of organic wastes. Bioresource Technol. 63: 91–99.CrossRefGoogle Scholar
  2. Cao, L.X., Xie, L.J., Xue, X.L., Tan, H.M., Liu, Y.H. and Zhou, S.N. (2007) Purification and characterization of alginate lyase from Streptomyces species strain A5 isolated from Banana Rhizosphere. J. Agric. Food Chem. 55: 5113–5117.PubMedCrossRefGoogle Scholar
  3. Castlehouse, H., Smith, C., Raab, A., Deacon, C., Meharg, A.A. and Feldmann, J. (2003) Biotransformation and accumulation of arsenic in soil amended with seaweed. Environ. Sci. Technol. 37: 951–957.PubMedCrossRefGoogle Scholar
  4. Eyras, M.C., Rostagno, C.M. and Defosse, G.E. (1998) Biological evaluation of seaweed composting. Compost Sci. Util. 6: 74–81.Google Scholar
  5. Fei, X.G. (2004) Solving the coastal eutrophication problem by large scale seaweed cultivation. Hydrobiologia 512: 145–151.CrossRefGoogle Scholar
  6. Iwamoto, Y., Araki, R., Iriyama, K., Oda, T., Fukuda, H., Hayashida, S. and Muramatsu, T. (2001) Purification and characterization of bifunctional alginate lyase from Alteromonas sp. strain no. 272 and its action on saturated oligomeric substrates. Biosci. Biotechnol. Biochem. 65: 133–142.PubMedCrossRefGoogle Scholar
  7. Iwasaki, K. and Matsubara, Y. (2000) Purification of alginate oligosaccharides with root growth-promoting activity toward lettuce. Biosci. Biotechnol. Biochem. 64: 1067–1070.PubMedCrossRefGoogle Scholar
  8. Ivanova, E.P., Bakunina, I.Y., Sawabe, T., Hayashi, K., Alexeeva, Y.V., Zhukova, N.V., Nicolau, D.V., Zvaygintseva, T.N. and Mikhailov, V.V. (2002) Two species of culturable bacteria associated with degradation of brown algae Fucus evanescens. Microb. Ecol. 43: 242–249.PubMedCrossRefGoogle Scholar
  9. Kawamoto, H., Horibe, A., Miki, Y., Kimura, T., Tanaka, K., Nakagawa, T., Kawamukai, M. and Matsuda, H. (2006) Cloning and sequencing analysis of alginate lyase genes from the marine bacterium Vibrio sp. O2. J. Mar. Biotechnol. 8: 481–490.CrossRefGoogle Scholar
  10. Matsushima, K., Minoshima. H., Kawanami, H., Ikushima, Y., Nishizawa, M., Kawamukai, A. and Hara, K. (2005) Decomposition reaction of alginic acid using subcritical and supercritical water. Ind. Eng. Chem. Res. 44: 9626–9630.CrossRefGoogle Scholar
  11. Miller, G.L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31: 426–428.CrossRefGoogle Scholar
  12. Mimura, H., Maeda, K. and Nagata, S. (1999) Chromatographic analysis of bean curd refuse decomposed by Bacillus sp. HR6. Biocontrol Sci. 4: 23–26.CrossRefGoogle Scholar
  13. Mimura, H. and Nagata, S. (1999) Physiological characteristics of Bacillus sp. HR6 in the process of decomposing bean curd refuse. Biocontrol Sci. 4: 105–108.CrossRefGoogle Scholar
  14. Moen, E., Horn, S. and Ostgaard, K. (1997) Alginate degradation during anaerobic digestion of Laminaria hyperborea stipes. J. Appl. Phycol. 9: 157-166.CrossRefGoogle Scholar
  15. Moen, E. and Ostgaard, K. (1997) Aerobic digestion of Ca-alginate gels studied as a model system of seaweed tissue degradation. J. Appl. Phycol. 9: 261–267.CrossRefGoogle Scholar
  16. Mormile, M.R., Romine, M.F., Garcia, T., Ventosa, A., Bailey, T.J. and Peyton, B.M. (1999) Halomonas campisalis sp nov., a denitrifying, moderately haloalkaliphilic bacterium. Syst. Appl. Microbiol. 22: 551–558.PubMedCrossRefGoogle Scholar
  17. Nagasawa, N., Mitomo, H., Yoshii, F. and Kume, T. (2000) Radiation-induced degradation of sodium alginate. Polym. Degrad. Stab. 69: 279–285.CrossRefGoogle Scholar
  18. Nagata, S. and Zhou, X. (2006) Analyses of factors to affect the bioassay system using luminescent bacterium Vibrio fischeri. J. Health Sci. 52: 9–16.CrossRefGoogle Scholar
  19. Ntougias, S., Zervakis, G..I., Ehaliotis, C., Kavroulakis, N. and Papadopoulou, K.K. (2006) Ecophysiology and molecular phylogeny of bacteria isolated from alkaline two-phase olive mill wastes. Res. Microbiol. 157: 376–385.PubMedCrossRefGoogle Scholar
  20. Ohno, M. and Critchley, A.T. (1993) Seaweed Cultivation and Marine Ranching, Kanagawa International Fisheries Training Center, Japan International Cooperation Agency (JICA), Tokyo.Google Scholar
  21. Schaumann, K. and Weide, G. (1990) Enzymatic degradation of alginate by marine fungi. Hydrobiologia 204/205: 589–596.CrossRefGoogle Scholar
  22. Skriptsova, A., Khomenko, V. and Isakov, V. (2004) Seasonal changes in growth rate, morphology and alginate content in Undaria pinnatifida at the northern limit in the Sea of Japan (Russia). J. Appl. Phycol. 16: 17–21.CrossRefGoogle Scholar
  23. Tang, J.C., Inoue, Y., Yasuta, T., Yoshida, S. and Katayama, A. (2003) Chemical and microbial properties of various compost products. Soil. Sci. Plant Nutr. 49: 273–280.CrossRefGoogle Scholar
  24. Tang, J.C., Wei, J.H., Maeda, K., Kawai, H., Zhou, Q., Hosoi-Tanabe, S. and Nagata, S. (2007) Degradation of seaweed wakame (Undaria pinnatifida) by composting process with inoculation of Bacillus sp. HR6. Biocontrol Sci. 12: 47–54.PubMedCrossRefGoogle Scholar
  25. Tang, J.C., Xiao, Y., Oshima, A., Kawai, H. and Nagata, S. (2008) Disposal of seaweed wakame (Undaria pinnatifida) in composting process by marine bacterium Halomonas sp. AW4. Int. J. Biotechnol. 10: 73–85.CrossRefGoogle Scholar
  26. Tiquia, S.M. and Tam, N.F.Y. (1998) Elimination of phytotoxicity during co-composting of spent pig-manure sawdust litter and pig sludge. Bioresource Technol. 65: 43–49.CrossRefGoogle Scholar
  27. Vendrame, W. and Moore, K.K. (2005) Comparison of herbaceous perennial plant growth in seaweed compost and biosolids compost. Compost Sci. Util. 13: 122–126.Google Scholar
  28. Ventosa, A., Nieto, J.J. and Oren, A. (1998) Biology of moderately halophilic aerobic bacteria. Microbiol. Mol. Biol. Rev. 62: 504–544.PubMedGoogle Scholar
  29. Waino, M., Tindall, B.J., Schumann, P. and Ingvorsen, K. (1999) Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int. J. Syst. Bacteriol. 49: 821–831.PubMedCrossRefGoogle Scholar
  30. Wong, T.Y., Preston, L.A. and Schiller, N.L. (2000) Alginate lyase: review of major sources and enzyme characteristics, structure–function analysis, biological roles, and applications. Ann. Rev. Microbiol. 54: 289–340.CrossRefGoogle Scholar
  31. Xu, X., Iwamoto, Y., Kitamura, Y., Oda, T. and Muramatsu, T. (2003) Root growth-promoting activity of unsaturated oligomeric uronates from alginate on carrot and rice plants. Biosci. Biotechnol. Biochem. 67: 2022–2025.PubMedCrossRefGoogle Scholar
  32. Yamada, N. (2001) Science of Seaweed Utilization. Seizando Press, Tokyo, Japan.Google Scholar
  33. Zhou, X., Okamura, H. and Nagata, S. (2006) Applicability of luminescent assay using fresh cells of Vibrio fischeri for toxicity evaluation. J. Health Sci. 52: 811–816.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Jing-Chun Tang
    • 1
  • Hideji Taniguchi
    • 2
  • Qixing Zhou
    • 1
  • Shinichi Nagata
    • 2
  1. 1.Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of EducationCollege of Environmental Science and Engineering, Nankai UniversityTianjinChina
  2. 2.Environmental Biochemistry Division, Research Center for Inland Seas, Organization of Advanced Science and TechnologyKobe UniversityKobeJapan

Personalised recommendations