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Integrated Pathway Analysis of Genome-Wide Expression Changes Associated with Serum-Free Suspension Adaptation of an Antibody-Producing Chinese Hamster Ovary (CHO) Cell Line

  • Yung-Shyeng TsaoEmail author
  • Ankit A. Merchant
  • Aaron Meyer
  • Zhong Liu
  • Marsha Smith
  • Diane Levitan
  • Eric Gustafson
Conference paper
  • 1.4k Downloads
Part of the Animal Cell Technology: Basic & Applied Aspects book series (ANICELLTECH, volume 16)

Abstract

Genome-wide expression profiles of antibody-producing CHO cells during suspension-adaptation into a serum-free medium have been characterized using CHO DNA microarrays. The transcriptome was analyzed with the RNA samples of cultures collected prior to and after adaptation. Over 500 genes and many major pathways were found to be profoundly affected by suspension adaptation. Among the down-regulated pathways were cell-cell adhesion, cell cycle, nucleotide synthesis, transcription, translation, vesiclular transport, microfilaments, and motor proteins. Among the up-regulated pathways were extracellular matrix, basement membrane, hypoxia signal pathway, antioxidant response elements, amino acid transporters as well as protein processing mechanisms in ER and Golgi. Most strikingly, the metabolic pathways were shifted to the de novo synthesis of fatty acids and sterols. The genes for fatty acid transport were significantly up-regulated and among them the mRNA of several key enzymes increased several hundred fold.

Keywords

Chinese Hamster Ovary Sterol Synthesis Cell Culture Process Gene Expression Fold Change Suspension Adaptation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Yung-Shyeng Tsao
    • 1
    Email author
  • Ankit A. Merchant
    • 2
  • Aaron Meyer
    • 3
  • Zhong Liu
    • 1
  • Marsha Smith
    • 4
  • Diane Levitan
    • 4
  • Eric Gustafson
    • 4
  1. 1.Protein Expression Technologies, BSPDMerck Research LaboratoriesUnionUSA
  2. 2.Protein Expression Technologies, GBSPDSchering-Plough Research InstituteUnionUSA
  3. 3.Bioengineering DepartmentUniversity of CaliforniaLos AngelesUSA
  4. 4.Functional Genomics, Discovery TechnologiesSchering-Plough Research InstituteKenilworthUSA

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