HU is one of the histone-like DNA binding proteins, which are involved in maintaining the nucleoid structure in bacteria. HU has also been shown to participate in transcriptional regulation of specific promoters. In this chapter, we provide an overview of the mechanism of HU action in the transcriptional regulation of the two promoters of the gal operon in E. coli by providing results of genetic, biophysical and biochemical experiments both in vivo and in vitro.
KeywordsAnti-parallel DNA Looping Atomic Force Microscopy gal Operon GalR HU
Much of the work described in this chapter is from the authors’ laboratory. We gratefully acknowledge the contributions of many of our past and present colleagues and collaborators too numerous to list. We are supported by the Intramural Research Program of the National Institutes of Health, the National Cancer Institute, the Center for Cancer Research. We thank Dr. Dhruba Chattoraj for suggestions and critical reading of the manuscript.
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