Advertisement

Antifouling Activity of Prodigiosin from Estuarine Isolate of Serratia marcescens CMST 07

  • K. Ananda Priya
  • S. Satheesh
  • B. Ashokkumar
  • P. Varalakshmi
  • G. Selvakumar
  • N. SivakumarEmail author
Chapter

Abstract

Microbial biofilms on the surfaces of man–made structures in the marine environment cause serious problems for marine industries. Currently used heavy metal-based toxic antifoulants has created environmental problem, which mandates the necessity of “eco-friendly” antifoulants. Marine-based microbial secondary metabolites are promising potential sources of nontoxic antifouling compounds. In the present study, we have investigated the antifouling potentials of bacterial red pigment prodigiosin extracted from Serratia marcescens CMST 07. Prodigiosin was showed high antifouling activity against marine fouling bacteria like Alteromonas sp. and Gallionella sp. minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the pigment was about 6.75 and 12.5 μg/ml respectively against Alteromonas sp. LD50 of prodigiosin against artemia (artemia toxicity study) was about 50 μg/ml. Prodigiosin significantly (P < 0.01) inhibits cyanobacterial adhesion on glass surface, which augments the possibility of using bacterial pigments as the source of antifouling compounds for controlling the fouling problem in the marine environments.

Keywords

Antifouling Bactericidal Prodigiosin Serratia marcescens 

References

  1. Abarzua S, Jakubowski S, Eckert S, Fuchs P (1999) Biotechnological investigation for the prevention of marine biofouling II. Blue-Green Algae as potential producers of biogenic agents for the growth inhibition of micro fouling organisms. Bot Mar 42:459–465CrossRefGoogle Scholar
  2. Basri DF, Fan SH (2005) The potential of aqueous and acetone extracts of galls of Quercus infectoria as antibacterial agents. Indian J Pharmacol 37(1):26–29CrossRefGoogle Scholar
  3. Bauer AW, Kirby MM, Sherris JC, Truck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496PubMedGoogle Scholar
  4. Bellas J (2006) Comparative toxicity of alternative antifouling biocides on embryos and larvae of marine invertebrates. Sci Total Environ 367:573–585PubMedCrossRefGoogle Scholar
  5. Bennett JW, Bentley R (2000) Seeing red: the story of prodigiosin. Adv Appl Microbiol 47:1–32PubMedCrossRefGoogle Scholar
  6. Berlanaga M, Vinas M (2000) Role of outer membrane in the accumulation of quinolones by Serratia marcescens. Can J Microbiol 46:716–721Google Scholar
  7. Bhosale SH, Nagle VL, Jagtap TG (2002) Antifouling potential of some marine organisms from India against species of Bacillus and Pseudomonas. Mar Biotechnol 4:111–118PubMedCrossRefGoogle Scholar
  8. Casullo de Araujo HW, Fukushima K, Campos Takaki GM (2010) Rodigiosin production by Serratia marcescens UCP 1549 using renewable-resources as a low cost substrate. Molecul 15:6931–6940CrossRefGoogle Scholar
  9. Clare AS (1996) Marine natural product anti foulants: status and potential. Biofouling 9:211–229CrossRefGoogle Scholar
  10. Depree C (2006) Developing natural solutions for a ‘foul’ problem. Wat Atmos 14(3):20–21Google Scholar
  11. Dobretsov S, Dahms HU, Qian PY (2006) A review: inhibition of biofouling by marine microorganisms and their metabolites. Biofoul 22:43–54CrossRefGoogle Scholar
  12. Egan S, James S, Holmstrom C, Kjelleberg S (2002) Correlation between pigmentation and antifouling compounds produced by Pseudoalteromonas tunicata. Environ Microbiol 4:433–442PubMedCrossRefGoogle Scholar
  13. Frank KT, Petrie B, Choi JS, Leggete WC (2005) Trophic cascades in a formerly cod-dominated ecosystem. Science 308:1621–1623PubMedCrossRefGoogle Scholar
  14. Fusetani N (2004) Biofouling and antifouling. Nat Prod Rep 21:94–104PubMedCrossRefGoogle Scholar
  15. Giri AV, Anandkumar N, Muthukumaran G, Pennathur G (2004) A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiol 4(11):1–10Google Scholar
  16. Greer SP, Iken KB, McClintock JB, Amsler CD (2003) Individual and coupled effects of echinoderm extracts and surface hydrophobicity on spore settlement and germination in the brown alga Hincksia irregularis. Biofoul 19:315–326CrossRefGoogle Scholar
  17. Hellio C, De La Broise D, Dufosse L, Gal Y, Bourgougnon N (2001) Inhibition of marine bacteria by extracts of macroalgae: Potential use for environmentally friendly antifouling paints. Mar Environ Res 52(3):231–247PubMedCrossRefGoogle Scholar
  18. IMO (2007) Revision of MARPOL annex VI and the NOx technical code. Input from the four subgroups and individual experts. IMO sub-committee on bulk liquids and gases (BLG). BLG 12/INF.10Google Scholar
  19. Kang YH, Kim JD, Kim BH, Kong DS, Han MS (2005) Isolation and characterization of a bio-agent antagonistic to the diatom Stephanodiscus hantzschii. J Appl Microbiol 98(5):1030–1038PubMedCrossRefGoogle Scholar
  20. Kubanek J, Whalen KE, Engel S, Kelly SR, Henkel TP, Fenical W, Pawlik JR (2002) Multiple defensive roles for triterpene glycosides from two Caribbean sponges. Oecologia 31:125–136CrossRefGoogle Scholar
  21. Lau SCK, Thiyagarajan V, Qian PY (2003) The bioactivity of bacterial isolates in Hong Kong waters for the inhibition of barnacle (Balanus amphitrite Darwin) settlement. J Exp Mar Biol Ecol 282:43–60CrossRefGoogle Scholar
  22. Long Richard A, Asfia Q, Faulkner D, Farooq JA (2003) 2-n-pentyl-4-quinolinol produced by a marine Alteromonas sp. and its potential ecological and biogeochemical roles. Appl Environ Microbiol 69:568–576PubMedCrossRefGoogle Scholar
  23. Maki JS, Rittschof D, Costlow JD, Mitchell R (1998) Inhibition of attachment of larval barnacles, Balanus amphitrite, by bacterial surface films. Marine Biol 97(2):199–206CrossRefGoogle Scholar
  24. Maldonado M (2007) Intergenerational transmission of symbiotic bacteria in oviparous and viviparous demo sponges, with emphasis on intr cytoplasmically-compartmented bacterial types. J Mar Biol Assoc UK 87:1701–1713CrossRefGoogle Scholar
  25. Marechal JP, Culioli G, Hellio C, Thomas-Guyon H, Callow ME, Clare AS, Ortalo-Magne A (2004) Seasonal variation in antifouling activity of crude extracts of the brown alga Bifurcaria bifurcate (Cystoseiraceae) against cyprids of Balanus amphitrite and the marine bacteria Cobetia marina and Pseudoalteromonas haloplanktis. J Exp Mar Biol Ecol 313:47–62CrossRefGoogle Scholar
  26. Mekhael R, Yousif SY (2009) The role of red pigment produced by Serratia marcescens as antibacterial and plasmid curing agent. J Duhok Univ 12(1):268–274Google Scholar
  27. Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols DE, McLaughlin JL (1982) Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 45:31–34CrossRefGoogle Scholar
  28. Montaner B, Perez-Tomas R (2003) The prodigiosins: a new family of anticancer drugs. Curr Can Drug Targ 3(1):57–65CrossRefGoogle Scholar
  29. Nakashima T, Kurachi M, Kato Y, Yamaguchi K, Oda T (2005) Characterization of bacterium isolated from the sediment Coast area of Omura Bay in Japan and several biological activities of pigment produced by this isolated. Microbiol Immunol 49:407–415PubMedGoogle Scholar
  30. Oclarit JM, Ohta S, Kamimura K, Yamaoka Y, Ikegami S (1994) Production of an antibacterial agent, O-aminophenol, by a bacterium isolated from a marine sponge Adocia sp. 3rd international marine biotechnology conference. IMBC, Programme, Abstracts and List of Participants. Tromsoe, Norway, p 98Google Scholar
  31. Paul VJ, Puglisi MP (2004) Chemical mediation of interactions among marine organisms. Nat Prod Rep 21:189–209PubMedCrossRefGoogle Scholar
  32. Paul VJ, Puglisi MP, Ritson-Williams R (2006) Marine chemical ecology. Nat Prod Rep 23:153–180PubMedCrossRefGoogle Scholar
  33. Qian PY, Xu Y, Fusetani N (2010) Natural products as antifouling compounds: recent progress and future perspectives. Biofoul 26(2):223–234CrossRefGoogle Scholar
  34. Railkin AI (2003) Marine biofouling: colonization processes and defenses. Boca Raton. CRC Press, Florida, p 320CrossRefGoogle Scholar
  35. Rappe MS, Giovannoni SJ (2003) The uncultured microbial majority. Ann Rev Microbiol 57:369–394CrossRefGoogle Scholar
  36. Rasmussen K, Ostgaard K (2003) Adhesion of the marine bacterium Pseudomonas sp. NCIMB 2021 to different hydrogel surfaces. Wat Res 37:519–524CrossRefGoogle Scholar
  37. Ren D, Sims JJ, Wood TK (2001) Inhibition of biofilm formation and swarming of Escherichia coli by (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2 (5H)- furanone. Environ Microbiol 3:731–736PubMedCrossRefGoogle Scholar
  38. Rittschof D, Branscomb ES, Costlow JD (1984) Settlement and behavior in relation to flow and surface in larval barnacles, Balanus amphitrite Darwin. J Exp Mar Biol Ecol 82:131–146CrossRefGoogle Scholar
  39. Samrot AV, Chandana K, Senthilkumar P, Narendra Kumar G (2011) Optimization of prodigiosin production by Serratia marcescens SU-10 and evaluation of its bioactivity. Int Res J Biotechnol 2(5):128–133Google Scholar
  40. Thomas KV, Brooks S (2010) The environmental fate and effects of antifouling paint biocides. Biofoul 26:73–88CrossRefGoogle Scholar
  41. Whitman WB, Coleman DC, Wiebe WJ (1998) Prokaryotes: the unseen majority. Proc Natl Acad Sci USA 95:6578–6583PubMedCrossRefGoogle Scholar
  42. Wieczorek SK, Todd CD (1998) Biofilm cues and larval settlement. Biofoul 12:81–118CrossRefGoogle Scholar
  43. Wilsanand V, Wagh AB, Bapuji M (1999) Antifouling activities of marine sedentary invertebrates on some macrofoulers. Ind J Mar Sci 28:280–284Google Scholar

Copyright information

© Springer India 2013

Authors and Affiliations

  • K. Ananda Priya
    • 1
  • S. Satheesh
    • 1
  • B. Ashokkumar
    • 2
  • P. Varalakshmi
    • 2
  • G. Selvakumar
    • 3
  • N. Sivakumar
    • 2
    Email author
  1. 1.Centre for Marine Science and TechnologyManonmaniam Sundaranar UniversityRajakkamangalam Kanyakumari DistrictIndia
  2. 2.School of BiotechnologyMadurai Kamaraj UniversityMaduraiIndia
  3. 3.Department of MicrobiologyAlagappa UniversityKaraikudiIndia

Personalised recommendations