Genetic Resources, Germplasm Characterisation/Evaluation and Maintenance

Chapter

Abstract

Gene pool serves as a tool for conceptualising the ability of plant populations to cross with the conspecific population and with those of other species. Classification of gene pool of Momordica spp. has been discussed here on the basis of hybridisation studies. M. dioica and M. subangulata in India are reported under endangered category. However, these reports are based on assumption and do not have the support of authentic fieldwork. A large collection of M. charantia is maintained in the national gene bank and by different organisations in India and other countries. A good representation of diversity in M. charantia has been assembled from the Western Ghats, India, though there are still a few grey areas to be explored more intensely. However, other species and areas need extensive coverage. Momordica species assume significance for conservation as crop relative, source of economic product of aesthetic and ecological interest, of potential horticultural value and as future raw material for the medicinal and pharmaceutical industry. Establishment of genetic reserves within protected areas must be attempted for conserving diversity in M. dioica and M. sahyadrica. Ex situ conservation in home gardens and on-farm conservation in tribal homesteads in forest pockets are viable options for conservation of Momordica gene pool as the taxa are still wild or semi-domesticate with high dependence on biotic agents for pollination and seed dispersal.

Keywords

Momordica gene pool Endangered In situ and ex situ conservation Genetic reserves 

References

  1. Anonymous (1997) ICUN red list of threatened plants, p 230Google Scholar
  2. Arora RK (1998) Plant genetic resources of north eastern region: diversity domestication trends, conservation and uses. Proc Indian Natl Sci Acad 63:175Google Scholar
  3. AVGRIS (2009) AVRDC vegetable genetic resources information system. http://203.64.245.173/avgris/. Accessed 11 Feb 2009
  4. Behera TK (2004) Heterosis in bitter gourd. In: Singh PK, Dasgupta SK, Tripathi SK (eds) Hybrid vegetable development. Haworth Press, New York, pp 217–221Google Scholar
  5. Behera TK, Gaikwad AB, Singh AK, Staub JE (2008a) Relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia L.). J Sci Food Agric 88:733–737CrossRefGoogle Scholar
  6. Behera TK, Singh AK, Staub JE (2008b) Comparative analysis of genetic diversity of Indian bitter gourd (Momordica charantia L.) using RAPD and ISSR markers for developing crop improvement strategies. Sci Hortic 115:209–217Google Scholar
  7. Behera TK, Joseph JK, Bharathi LK, Karuppaiyan R (2011) Momordica. In: Kole C (ed) Wild crop relatives: genomic and breeding resources. Springer, Netherlands. ISBN 978-3-642-20449-4, pp 217–246Google Scholar
  8. Bettencourt E, Konopka J (1990) Directory of crop germplasm collections. 4. Vegetables. IBPGR, Rome, p 147Google Scholar
  9. Bharathi LK, Naik G, Dora DK (2005) Genetic divergence in spine gourd (Momordica dioica Roxb.). Veg Sci 32:179–181Google Scholar
  10. Bharathi LK, Munshi AD, Behera TK, Joseph JK, Vishalnath, Bisht IS (2010) Genetic resources of spine gourd (Momordica dioica Roxb. Ex Willd.): an underexplored nutritious vegetable from tribal regions of eastern India. Plant Genet Resour 8:225–228Google Scholar
  11. Bharathi LK, Munshi AD, Behera TK, Vinod, Joseph JK, Bhat KV, Das AB, Sidhu AS (2012a) Production and preliminary characterization of novel inter-specific hybrids derived from Momordica species. Curr Sci 103:178–186 Google Scholar
  12. Bharathi LK, Parida SK, Munshi AD, Behera TK, Raman KV, Mohapatra T (2012b) Molecular diversity and phonetic relationship of Momordica spp. of Indian occurrence. Genet Resour Crop Evol 59:937–948CrossRefGoogle Scholar
  13. Bootprom N, Songsri P, Suriharn B, Chareonsap P, Sanitchon J, Lertrat K (2012) Molecular diversity among selected Momordica cochinchinensis (Lour.) Spreng accessions using RAPD markers. SABRAO J Breed Genet 44:406–417Google Scholar
  14. Borokini TI, Okere AU, Giwa AO, Daramola BO, Odofin WT (2010) Biodiversity and conservation of plant genetic resources in field Genebank of the National Centre for Genetic Resources and Biotechnology, Ibadan, Nigeria. Int J Biodiversity Conserv 2:37–50Google Scholar
  15. Botau D, Ciulca S, Frant A (2010) The variability study of some quantitative traits at Momordica charantia L. Bull UASVM Hortic 67:230–234Google Scholar
  16. Changyuan Z, Ni S, Kailin H (2005) RAPD analysis in genetic relationship among varieties of balsam pear. Mol Plant Breed 3:515–519Google Scholar
  17. Chung SM, Staub JE (2004) Consensus chloroplast primer analysis: a molecular tool for evolutionary studies in Cucurbitaceae. In: Lebeda A, Paris HS (eds) Proceedings of Cucurbitaceae 2004, 8th EUCARPIA meeting on Cucurbit genetics and breeding. Palacky University, Olomouc, Czech Republic, pp 477–483Google Scholar
  18. Dalamu, Behera TK, Gaikwad AB, Swati S, Bharadwaj C, Munshi AD (2012) Morphological and molecular analyses define the genetic diversity of Asian bitter gourd (Momordica charantia L.) Australian J Crop Sci 6:261–267Google Scholar
  19. Dey SS, Singh AK, Chandel D, Behera TK (2006) Genetic diversity of bitter gourd (Momordica charantia L.) genotypes revealed by RAPD markers and agronomic traits. Sci Hortic 109:21–28CrossRefGoogle Scholar
  20. Diez MJ, Pico B, Nuez F (2002) Cucurbit genetic resources in Europe. Ad hoc meeting, 19 Jan 2002, Adana, Turkey. International Plant Genetic Resources Institute, Rome, ItalyGoogle Scholar
  21. Dwivedi SN (1999) Traditional health care among tribals of Rewa district of Madhya Pradesh with special reference to conservation of endangered and vulnerable species. J Econ Taxon Bot 2:315–320Google Scholar
  22. Gaikwad AB, Behera TK, Singh AK, Chandel D, Karihaloo JL, Staub JE (2008) AFLP analysis provides strategies for improvement of bitter gourd (Momordica charantia). HortScience 43:127–133Google Scholar
  23. Ghosh SP, Kalloo G (2000) Genetic resources of indigenous vegetables and their uses in South Asia. Technical Bulletin No 4, IIVR, Varanasi, UP, India, pp 36–37Google Scholar
  24. Harlan JR, de Wet JMT (1971) Toward a rational classification of cultivated plants. Taxon 20:509–517CrossRefGoogle Scholar
  25. Harriet G (2002) Conservation and sustainable use of medicinal plants in Ghana medicinal plant accession data. Aburi Botanic Garden, Botanic Gardens Conservation International, Ghana, 53 pGoogle Scholar
  26. Heywood V (1998) Conservation and sustainable use of wild species of importance in Human activities. In: Proceedings of international conservation conference, The Jawaharlal Nehru Tropical Botanic Garden and Research Institute (TBGRI), ThiruvananthapuramGoogle Scholar
  27. Horvath L (2002) Status of the national cucurbit collection in Hungary. In: Diez MJ, Pico B, Nuez F (eds) First ad hoc meeting on cucurbit genetic resources, Adana, Turkey, 30 pGoogle Scholar
  28. Islam MS, Khan S (1998) Pollen viability of Momordica dioica Roxb. as affected by storage period and temperature. Bangladesh J Bot 27:153–155Google Scholar
  29. Islam MR, Hossain MS, Bhuiyan MSR, Hasan GN, Syed A (2010) Multivariate analysis of bitter gourd (Momordica charantia L.). Middle-East J Sci Res 5:86–90Google Scholar
  30. Jha UC, Ujawane RG (2002) Collection, evaluation and utilization of Momordica species. Contributed paper in the international seminar on vegetables at BangaloreGoogle Scholar
  31. Joseph JK (2005) Studies on ecogeography and genetic diversity of the genus Momordica L. in India. Dissertation, Mahatma Gandhi University, KottayamGoogle Scholar
  32. Joseph JK, Antony VT (2007) A quantitative analysis of genetic erosion in the genus Momordica L. in South Peninsular India. Indian J Plant Genet Resour 20:186–192Google Scholar
  33. Joseph JK, Antony VT (2009) Collection and preliminary evaluation of small bitter gourd (Momordica charantia L.) a relict vegetable of Southern Peninsular India. Genet Resour Crop Evol 56:99–104CrossRefGoogle Scholar
  34. Joseph JK, Antony VT (2011) Developing descriptors for dioecious Momordica species for germplasm characterization and evaluation. Indian J Plant Genet Resour 24:31–39Google Scholar
  35. Joshi V, Gautham PL, Bhagmal, Sharma GD, Kochhar S (2002) Conservation and use of underutilized crops. An Indian perspective. In: Engles JMM, Ramanatha Rao V, Brown AHD, Jackson MT (eds) Managing plant genetic diversity, IPGRI, RomeGoogle Scholar
  36. Karlova K (2008) Cucurbitaceae genetic resources in the Czech gene bank. Current status of the collection. In: Pitrat M (ed) 9th EUCARPIA meeting on genetics and breeding of Cucurbitaceae, INRA, Avignon, France, 21–24 May 2008Google Scholar
  37. Kole C, Olukolu B, Kole P, Abbott AG (2009) Characterization of a Momordica charantia germplasm with AFLP and SSR markers. In: Molecular markers in horticulture symposium, Oregon State University, Corvallis, 29 July–1 Aug 2009Google Scholar
  38. Kole C, Olukolu BA, Kole P, Abbott AG (2010a) Towards phylomedomics with bitter melon (Momordica charantia L.) as a model. In: International conference on the status of plant and animal genome research, San Diego, CA, USA, 9–13 Jan 2010, p 164Google Scholar
  39. Kucuk A, Abak, K, Sari N (2002) Cucurbit genetic resources collections in Turkey. In: Diez MJ, Pico B, Nuez F (eds) First ad hoc meeting on cucurbit genetic resources, Adana, Turkey, p 46Google Scholar
  40. Lebeda A, Widrlechner MP, Staub J, Ezura H, Zalapa J, Kristkova E (2006) Cucurbits (Cucurbitaceae; Cucumis spp., Cucurbita spp., Citrullus spp.) In: Ram J Singh (ed) Genetic resources, chromosome engineering and crop improvement, vol 3-Vegetable crops. CRC Press, Taylor and Francis group, Boca Raton, pp 271–376Google Scholar
  41. Liao PC, Tsai CC, Chou CH, Chiang YC (2012) Introgression between cultivars and wild populations of Momordica charantia L. (Cucurbitaceae) in Taiwan. Int J Mol Sci 13:6469–6491PubMedCrossRefGoogle Scholar
  42. Marr KL, Xia YM, Bhattarai NK (2004) Allozyme, morphological and nutritional analysis bearing on the domestication of Momordica charantia L. (Cucurbitaceae). Econ Bot 58:435–455CrossRefGoogle Scholar
  43. Paul A, Bandyopadhyay S, Acharyya P, Raychaudhuri SS (2010) Studies on genetic diversity of twelve accessions of Momordica charantia L. using morphological, RAPD and Scar markers. Asian J Plant Sci 9:471–478CrossRefGoogle Scholar
  44. Piskunova T (2002) Status of the cucurbit collections in Russia. In: Diez MJ, Pico B, Nuez F (eds) First ad hoc meeting on cucurbit genetic resources. Adana, Turkey, 19 Jan 2002, pp 37Google Scholar
  45. Promote P, Pornthip P, Numuen C (2011) Phenotypic diversity and classification of Thai bitter melon (Momordica charantia L.) land races from three provinces in central region of Thailand. J Agric Technol 7:849–856Google Scholar
  46. Raj NM, Prasanna KP, Peter KV (1993) Bitter Gourd. In: Kalloo G, Bergh BO (eds) Genetic improvement of vegetable plants. Pergamon Press, Oxford, UK, pp 239–246Google Scholar
  47. Rajashekaran PE, Sunitha B, Joseph JK, Eapen KP, Antony VT (2010) Pollen cryopreservation feasibility studies in Momordica dioica and M. sahyadrica. IUP J Genet Evol 3:53Google Scholar
  48. Rajashekaran PE, Sunitha B, Joseph JK, Eapen KP, Antony VT (2011) In vitro multiplication and conservation of Wild Momordica sahyadrica. IUP J Biotechnol 6:50–56Google Scholar
  49. Ram D, Srivastava U (1999) Some lesser known minor cucurbitaceous vegetables: their distribution, diversity and use. Indian J Plant Genet Resour 12:307–316Google Scholar
  50. Ram D, Banerjee MK, Pandey S, Srivastava U (2001) Collection and evaluation of kartoli (Momordica dioica Roxb. Ex. Willd.) Indian J Plant Genet Resour 14:114–116Google Scholar
  51. Ram D, Kumar S, Banerjee MK, Singh B, Singh S (2002) Developing bitter gourd (Momordica charantia L.) populations with very high proportion of pistillate flowers. Cucurbit Genet Coop Rep 25:65–66Google Scholar
  52. Rasul MG, Okubo H (2002) Genetic diversity in teasel gourd (M. dioica Roxb.) Bangladesh. J Plant Breed Genet 15:7–15Google Scholar
  53. Rasul MG, Hiramatsu M, Okubo H (2004) Morphological and physiological variation in Kakrol (M. dioica Roxb.) J Fac Agric Kyushu Univ 49:1–11Google Scholar
  54. Rasul MG, Hiramatsu M, Okubo H (2007) Genetic relatedness (diversity) and cultivar identification by randomly amplified polymorphic DNA (RAPD) markers in teasel gourd (Momordica dioica Roxb.). Sci Hortic 111:271–279CrossRefGoogle Scholar
  55. Rathod V, Narasegowda NC, Papanna N, Simon L (2008) Evaluation of genetic diversity and genome fingerprinting of bitter gourd genotypes (Momordica charantia L.) by morphological and RAPD markers. Int J Plant Breed 2:79–84Google Scholar
  56. Robinson RW, Decker-Walters DS (1997) Cucurbits. CABI Publishers, Wallingford, pp 97–101Google Scholar
  57. Schaefer H, Renner SS (2010) A three-genome phylogeny of Momordica (Cucurbitaceae) suggests seven returns from dioecy to monoecy and recent long-distance dispersal to Asia. Mol Phylogenet Evol 54:553–560PubMedCrossRefGoogle Scholar
  58. Sikdar B, Bhattacharya A, Mukherjee A, Banerjee A, Ghosh E, Ghosh B, Roy SC (2010) Genetic diversity in important members of Cucurbitaceae using isozyme, RAPD and ISSR markers. Biol Plantarum 54:135–140CrossRefGoogle Scholar
  59. Singh AK, Behera TK, Chandel D, Sharma P, Singh NK (2007) Assessing genetic relationships among bitter gourd (Momordica charantia L.) accessions using inter simple sequence repeat (ISSR) markers. J Hortic Sci Biotechnol 82:217–222Google Scholar
  60. Sirohi PS, Choudhury B (1983) Diallel analysis for variability in bitter gourd (Momordica charantia L.) Indian J Agric Sci 53:8–88Google Scholar
  61. Srivastava U, Mahajan RK, Gangopadyay KK, Singh M, Dhillon BS (2001) Minimal descriptors of agri-horticultural crops. Part-II: Vegetable crops. NBPGR, New Delhi, India, pp 61–66Google Scholar
  62. Vishalnath, Mandal S, Bharathi LK, Naik G, Singh HS (2008a) CHTG 2 (Neelachal Gaurav): a soft-seeded teasel gourd variety. ICAR News 14:15Google Scholar
  63. Vishalnath, Mandal S, Bharathi LK, Naik G, Singh HS (2008b) CHSG 28 (Neelachal Shree): an elegant spine gourd variety. ICAR News 14:15Google Scholar
  64. Walters TW, Decker-Walters DS (1988) Balsam pear (Momordica charantia, Cucurbitaceae). Econ Bot 42:286–288CrossRefGoogle Scholar
  65. Walter R, Epperson BK (2001) Geographic pattern of genetic variation in Pinus resinosa: area of greatest diversity is not the origin of postglacial populations. Mol Ecol 10:103–111Google Scholar
  66. Wang SZ, Pan L, Hu K, Chen CY, Ding Y (2010) Development and characterization of microsatellite markers in Momordica charantia (Cucurbitaceae). Am J Bot 97:e75–e78PubMedCrossRefGoogle Scholar
  67. Yadav M, Singh DB, Chaudhary R, Singh D (2008) Genetic variability in bitter gourd (Momordica charantia L.). J Hortic Sci 3:35–38Google Scholar
  68. Zuberi ML, Biswas A (1998) Biodiversity degradation in Bangladesh: wild kakrol (Momordica dioica Roxb.) an endangered species. Asia Pac J Environ Develop 5:66–74Google Scholar

Copyright information

© Springer India 2013

Authors and Affiliations

  1. 1.HorticultureCentral Horticultural Experiment StationBhubaneswarIndia
  2. 2.Economic BotanyNational Bureau of Plant Genetic ResourcesThrissurIndia

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