Biochemical Engineering for 2001 pp 331-334 | Cite as
Production of t-PA by Recombinant Mouse Fibroblast Cell Cultures in a Perfusion Bioreactor
Abstract
As heart disease is known to be one of the leading cause of human death, t-PA(tissue-type plasminogen activator) has been received great attention as a promising thrombolytic agent and also as one of the first commercial products via the recombinant DNA-technology. Breaking up blood clot is the essential treatment of restoring the normal flow of blood in the acute myocardial infarction cases [1]. Thrombolytic agents activates plasminogen to form plasmin, which dissolves fibrin-the main component of blood clots. Plasminogen is a pro-enzyme that naturally exists in blood as free form and/or plasminogen-fibrin complex by binding to blood clots, more specifically fibrin.
Keywords
tissue-type plasminogen activator (t-PA) fibroblast cell perfusion bioreactor collagen microcarrierPreview
Unable to display preview. Download preview PDF.
References
- [1]Chien K, Meidell R, Gerard R (1987) Tissue plasminogen activator: From molecular biology to myocardial infarction. Amer. J. of Med. Sci., 292, 201–207.CrossRefGoogle Scholar
- [2]Geiger M, Binder B (1987) Tissue-type plasminogen activator and urokinase: Differences in the reaction pattern with active-site titrant 4-methyumbelliferyl-p guanidinobenzoate hydrochloride. Biochimica Biophysica Acta, 912, 34–40CrossRefGoogle Scholar
- [3]Kretzmer G, Scrugerl K (1991) Response of mammalian cells to shear stress. Appl Microbiol Biotechnol, 34, 613–616.PubMedCrossRefGoogle Scholar