Summary
Squid ink, which has little commercial use and is usually discarded, was extracted with Tris-HCl buffer (pH 6.8). The extract was fractionated using DEAE Sephacel ion-exchange chromatography and Sephacryl S-300gel filtration to give a peptidoglycan fraction that exhibited strong antitumor activity against Meth-A fibrosarcoma in BALB/c mice following intraperitoneal injection. The fraction contained 17.8% peptide, 51.2% polysaccharide moiety, and 30.3% pigment. Furthermore three fucose (Fuc)-rich glycosaminoglycans (GAGs), illexin (ILX)-A, -B, and -C (MW 50000, 50000–80000, and 80000), were isolated from the peptidoglycan fraction, using Actinase E digestion, DEAE Sephadex A-50 ion-exchange chromatography, and Sephacryl S-300gel filtration. They gave a single band on the electrophoresis using cellulose acetate membrane and contained equimolar ratios of Fuc, glucuronic acid (GlcA), and N-acetylgalactosamine (GalNAc). These GAGs were not digested with any glycosaminoglycanases, but each gave tri- and hexasaccharides with mild acid hydrolysis. On the basis of spectral data, chemical compositional analysis, and chemical and enzymatic degradation analyses of GAGs and the pyridylaminated (PA) oligosaccharides, the unique repeating branched-structure of the GAGs was determined to be [-3GlcAβl-4(GalNAcαl-3)Fucαl-]n.
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References
Takaya Y, Uchisawa H, Matsue H, Okuzaki B, Narumi F, Sasaki J, Ishida K (1991) An investigation of the antitumor peptidoglycan fraction from squid ink. Biol Pharmacol Bull 17:846–849
Takaya Y, Uchisawa H, Hanamatsu K, Narumi F, Okuzaki B, Matsue H (1994) Novel fucose-rich glycosaminoglycans from squid ink bearing repeating unit of trisaccharide structure (-6GalNAcα1–3GlcAβ1–3Fucαl-)n. Biochem Biophys Res Commun, 198:560–567
Anno K, Kawai Y, Seno N (1964) Isolation of chondroitin from squid skin. Biochim Biophys Acta 83:348–349
Kawai Y, Seno N, Anno K (1966) Chondroitin polysulfate of squid cartilage. J Biochem (Tokyo) 60:317–321
Tanaka K (1978) Physicochemical properties of chondroitin sulfate. J Biochem (Tokyo) 83:647–653
Ohhashi Y, Hasumi F, Mori Y (1984) Comparative study on glycosaminoglycans synthesized in peripheral and peritoneal polymorphonuclear leucocytes from guinea pigs. Biochem J 217:199–207
Vieira RP, Mourao PAS (1988) Occurrence of a unique fucose-branched chondroitin sulfate in the body wall of a sea cucumber. J Biol Chem 263:18176–18183
Kariya Y, Watabe S, Hashimoto K, Yoshida K (1990) Occurrence of chondroitin sulfate E in glycosaminoglycan isolated from the body wall of a sea cucumber Stichopus japonicus. J Biol Chem 265:5081–5085
Matsue H, Takaya Y, Uchisawa H, Okuzaki B, Narumi F, Sasaki J, Ishida K (1996) Antitumor peptidoglycan having new carbohydrate chain originated from squid ink (in Japanese). Marine Biotechnol (in press)
Mochizuki A (1979) An antiseptic effect of cuttlefish ink (in Japanese). Bull Jpn Soc Sci Fish 45:1401–1403
Mimura T, Maeda K, Terada T, Oda Y, Morisita K, Aonuma S (1985) Studies on biological activities of melanin from marine animals. III. Chem Pharmacol Bull 33:2052–2060
Takaya Y, Uchisawa H, Narumi F, Matsue H (1996) Illexin A, B, and C from squid ink should have a branched structure. Biochem Biophys Res Commun 226:335–338
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© 1997 Springer Japan
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Matsue, H. et al. (1997). Antitumor Peptidoglycan with New Carbohydrate Structure from Squid Ink. In: Ohigashi, H., Osawa, T., Terao, J., Watanabe, S., Yoshikawa, T. (eds) Food Factors for Cancer Prevention. Springer, Tokyo. https://doi.org/10.1007/978-4-431-67017-9_65
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DOI: https://doi.org/10.1007/978-4-431-67017-9_65
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