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Muse Cells pp 69-101 | Cite as

Protocols for Isolation and Evaluation of Muse Cells

  • Kazuki Tatsumi
  • Yoshihiro Kushida
  • Shohei Wakao
  • Yasumasa Kuroda
  • Mari DezawaEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1103)

Abstract

This chapter provides the detailed method for isolation of Muse cells and evaluation of their pluripotency. The basic population of Muse cells is cultured mesenchymal stem cells such as bone marrow-mesenchymal stem cells, fibroblasts, and adipose-derived stem cells. The detailed method for handling mesenchymal stem cells is also provided in this protocol.

Keywords

MSCs Fibroblasts Adipose-derived stem cells Serum lot check Anti-SSEA-3 antibody Cell sorting FACS Clusters 

Copyright information

© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Kazuki Tatsumi
    • 1
    • 2
  • Yoshihiro Kushida
    • 1
  • Shohei Wakao
    • 1
  • Yasumasa Kuroda
    • 1
  • Mari Dezawa
    • 1
    Email author
  1. 1.Department of Stem Cell Biology and HistologyTohoku University Graduate School of MedicineSendaiJapan
  2. 2.Analytical Research Department, Technology Development Unit, Regenerative Medicine DivisionLife Science Institute, Inc.TokyoJapan

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