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Muse Cells pp 255-271 | Cite as

Artificial Pigmented Human Skin Created by Muse Cells

  • Takeshi Yamauchi
  • Kenshi YamasakiEmail author
  • Kenichiro Tsuchiyama
  • Setsuya Aiba
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1103)

Abstract

The skin composes physiological and chemical barrier and renews skin component cells throughout the human life. Melanocytes locate in the basal layer of the epidermis and produce melanin to protect the skin from ultraviolet. Melanin plays key roles in determining human skin and hair color. Melanocyte dysfunction observed in albinism and vitiligo not only causes cosmetic problems but also increases risk of skin cancer. As rejuvenate therapy, embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have been reported to generate melanocytes. Other than ES and iPS cells, human skin tissues maintain pluripotent stem cells, named multilineage-differentiating stress-enduring (Muse) cells. We employ Muse cells isolated from human fibroblasts and adipose tissue to differentiate into melanocytes (Muse-MC). Muse-MC express melanocyte-related molecules, such as tyrosinase and DCT, and show tyrosinase activity. We also succeeded to differentiate Muse cells into fibroblasts and keratinocytes and created three-dimensional (3D) reconstituted skin with Muse cell-derived melanocytes, fibroblasts, and keratinocytes. The 3D reconstituted skin of Muse cell-derived cells coordinately showed epidermis layers and Muse-MC localized in the basal layer of the epidermis. Thus Muse cells in the human skin can be a source of rejuvenation medicine for the skin reconstruction.

Keywords

Melanocyte Fibroblast Keratinocyte Melanin 3D skin a-MSH 

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Copyright information

© Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Takeshi Yamauchi
    • 1
  • Kenshi Yamasaki
    • 1
    Email author
  • Kenichiro Tsuchiyama
    • 1
  • Setsuya Aiba
    • 1
  1. 1.Department of DermatologyTohoku University Graduate School of MedicineSendaiJapan

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