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Biopharmaceutical Evaluation of Intermolecular Interactions by AUC-SE

  • Shuntaro Saito
  • Susumu Uchiyama
Chapter

Abstract

Analytical ultracentrifugation sedimentation equilibrium (AUC-SE) is a useful technique to investigate the weak reversible intermolecular interactions among proteins in solution. It provides biophysical information such as the average apparent molecular weight, stoichiometry, and association constant of associating proteins. Several studies of intermolecular interaction in biopharmaceuticals by AUC-SE are introduced in this chapter. AUC-SE also provides the second virial coefficient (B2), which represents the type, i.e., repulsive and attractive, and a magnitude of intermolecular interactions. The B2 values obtained from the protein solution at low concentrations showed good correlation with aggregation and viscosity of MAb at high concentrations, indicating that B2 can be an effective indicator of aggregation propensity and viscosity. These findings suggest that AUC-SE provides clues to understand the self-association in biopharmaceuticals and to establish effective manufacturing process, formulation, and administration of biopharmaceuticals.

Keywords

Analytical ultracentrifugation sedimentation equilibrium Self-association Colloidal stability Aggregation Viscosity Second virial coefficient Antibody 

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Copyright information

© Springer Japan 2016

Authors and Affiliations

  1. 1.Biologics Technology Research LaboratoriesDaiichi Sankyo Co., Ltd.Hiratsuka-shiJapan
  2. 2.Department of Biotechnology, Graduate School of EngineeringOsaka UniversitySuitaJapan

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