Advertisement

Experimental Design and Practical Aspects

  • Fumio Arisaka
  • Susumu Uchiyama
Chapter

Abstract

This chapter summarizes basic experimental setup and precautions to be made for successful measurements in analytical ultracentrifugation. There are two types of ultracentrifuge, XL-A and XL-I from Beckman Coulter Co. Ltd. A new ultracentrifuge, CFA analytical ultracentrifuge, is described in Chap.  3.

Keywords

Analytical ultracentrifugation Sedimentation velocity Sedimentation equilibrium Rotor Cell 

References

  1. Brautigam CA, Padrick SB, Schuck P (2013) Multi-signal sedimentation velocity analysis with mass conservation for determining the stoichiometry of protein complexes. PLoS ONE 8(5):e62694CrossRefPubMedPubMedCentralGoogle Scholar
  2. Harding SE, Rowe AJ, Horton JC (eds) (1992) Analytical ultracentrifugation in biochemistry and polymer science. Royal Society of Chemistry, CambridgeGoogle Scholar
  3. Laue TM (1996) Beckman application information A-1821A, pp 1–18Google Scholar
  4. Schuster TM, Laue TM (eds) (1994) Modern analytical ultracentrifugation: acquisition and interpretation of data for biological and synthetic polymer systems. Birkhäuser, BostonGoogle Scholar
  5. Scott D, Harding SE, Rowe A (eds) (2005) Analytical ultracentrifugation: techniques and methods. Royal Society of Chemistry, CambridgeGoogle Scholar

Copyright information

© Springer Japan 2016

Authors and Affiliations

  1. 1.Life Science Research Center, College of Bioresource ScienceNihon UniversityFujisawaJapan
  2. 2.Department of Biotechnology, Graduate School of EngineeringOsaka UniversitySuitaJapan
  3. 3.Department of Bioorganization ResearchOkazaki Institute for Integrative BioscienceOkazakiJapan

Personalised recommendations