Important and Essential Theoretical Aspects of AUC

  • Susumu UchiyamaEmail author
  • Fumio Arisaka


Analytical ultracentrifugation (AUC) is a powerful method to reveal biophysical behavior of solute in solution. AUC has a long history and is based on well-established and concrete thermodynamic and hydrodynamic theory. AUC provides valuable parameters such as the sedimentation and diffusion coefficients, from which molar mass and information on hydrodynamic shape and solvation of the solute can be derived. Here, important and essential theoretical aspects of AUC are described.


Analytical ultracentrifugation Sedimentation equilibrium Sedimentation velocity Theory 


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Copyright information

© Springer Japan 2016

Authors and Affiliations

  1. 1.Department of Biotechnology, Graduate School of EngineeringOsaka UniversitySuitaJapan
  2. 2.Department of Bioorganization ResearchOkazaki Institute for Integrative BioscienceOkazakiJapan
  3. 3.Life Science Center, College of Bioresource ScienceNihon UniversityFujisawaJapan

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