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Important and Essential Theoretical Aspects of AUC

  • Susumu Uchiyama
  • Fumio Arisaka
Chapter

Abstract

Analytical ultracentrifugation (AUC) is a powerful method to reveal biophysical behavior of solute in solution. AUC has a long history and is based on well-established and concrete thermodynamic and hydrodynamic theory. AUC provides valuable parameters such as the sedimentation and diffusion coefficients, from which molar mass and information on hydrodynamic shape and solvation of the solute can be derived. Here, important and essential theoretical aspects of AUC are described.

Keywords

Analytical ultracentrifugation Sedimentation equilibrium Sedimentation velocity Theory 

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Copyright information

© Springer Japan 2016

Authors and Affiliations

  1. 1.Department of Biotechnology, Graduate School of EngineeringOsaka UniversitySuitaJapan
  2. 2.Department of Bioorganization ResearchOkazaki Institute for Integrative BioscienceOkazakiJapan
  3. 3.Life Science Center, College of Bioresource ScienceNihon UniversityFujisawaJapan

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