Innovations in Measuring Cellular Mechanics
This article describes several novel mechanical methods for elucidating cellular responses to different types of mechanical loading (adhesive, pulling, pushing, shearing, and stretching forces). Understanding how cells deform and transmit stresses into the cell is important for gene expression, cytoskeletal remodeling, and focal adhesion reorganization and crucial for a variety of higher fundamental cell functions including cell division, motility, and differentiation. Introducing these unique methods of measuring and understanding cellular mechanics, therefore, provides a valuable platform for cell biology research.
KeywordsMagnetic tweezer Magnetic twisting cytometry Traction force microscopy Cell poking Plate rheometer Nano-scale tracking
We thank Dr. Ben Fabry for helpful discussions and Dr. Vicky Jackiw for proofreading the manuscript. This work was supported in part by grants from Deutscher Akademischer Austauschdienst (DAAD) and Deutsche Forschungsgemeinschaft (DFG).
- Bonakdar N, Schilling A, Sprrer M, Lennert P, Mainka A, Winter L, Walko G, Wiche G, Fabry B, Goldmann WH (2015) Determining the mechanical properties of plectin in mouse myoblasts and keratinocytes. Exp Cell Res 331:331–337Google Scholar
- Daily B, Elson EL, Zahalak GL (1984) Cell poking. Determination of the eleastic area compressibility modulus of the erythrocyte membrane. Biophys J 45:671–82Google Scholar
- Duszyk M, Schwab B 3rd, Zahalak GL, Qian H, Elson EL (1989) Cell poking: quantitative analysis of indentation of thick viscoelastic layers. Biophys J 55:683–90Google Scholar
- Goldmann WH, Alonso JL et al (2000) Cell shape control and mechanical signaling through the cytoskeleton. In: Carraway KL, Carraway CAC (eds) Cytoskeleton: signaling and cell regulation: Chapter 11. Oxford University Press, OxfordGoogle Scholar
- Hill AV (1965) Trails and trials in physiology. E. Arnold, London, pp 14–15Google Scholar