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cDNA probes for the detection of pestiviruses

  • Catherine Cruciere
  • L. Bakkali
  • Monique Gonzague
  • E. Plateau
Conference paper
Part of the Archives of Virology book series (ARCHIVES SUPPL, volume 3)

Summary

Probes were prepared from genomic RNA of Hog Cholera Virus (HCV) after synthesis of cDNA and cloning. Six probes were selected according to their place on the viral genome determined by sequencing and comparison with BVDV sequence. These probes were hybridized with two strains of HCV (Alfort and Nord), two strains of Bovine Viral Diarrhea (BVDV) (NADL, New York) and four strains of Border Disease (BD) (Lyon 1, Lyon 2, Aveyron, IEMVT). This panel of six probes seem to be able to differentiate pestiviruses but some differences rely only on slight intensity of the hybridization.

Key words

cDNA probes pestiviruses diagnosis distinction 

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Catherine Cruciere
  • L. Bakkali
  • Monique Gonzague
  • E. Plateau
    • 1
  1. 1.Centre National d’Etudes Vétérinaires et Alimentaires, Laboratoire Central de Recherches Vétérinaires, (C.N.E.V.A./L.C.R.V.)Maisons-Alfort CedexFrance

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