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Tracking of Autologous VSOP-Labeled Mesenchymal Stem Cells in the Sheep Brain Using 3.0 T MRI

  • Antje Y. Dreyer
  • Donald Lobsien
  • Claudia Pösel
  • Karl-Titus Hoffmann
  • Björn Nitzsche
  • Matthias Findeisen
  • Albrecht Stroh
  • Johannes Boltze
Chapter

Abstract

Assessment of biodistribution and monitoring of cell migration processes in vivo are essential for the safety of novel cell-based therapies for ischemic stroke and early-stage clinical trials, but are mainly lacking investigation in large animal models which are closer to the situation found in human patients. This chapter reports a series of experiments which establish a MRI-sensitive labeling procedure for autologous ovine mesenchymal stem cells (MSC) and the assessment of in vivo and in vitro detection limits of the cells at 3.0 T. Cell migration was monitored after intravenous transplantation following experimental stroke in sheep. Cell detection was feasible at 3.0 T with detection limits defined at 500 cells in vitro and 1,000 cells after local stereotaxic administration in vivo. No signs for MSC homing toward the ischemic lesion were observed after systemic cell delivery. Iron-containing cells were identified in the lung and skin wounds, but not in brain parenchyma after intravenous cell delivery. These findings are in contrast to results obtained in small animal models and may indicate significant differences of MSC behavior in large organisms. They also revealed the necessity for sensitivity-enhanced MRI sequences for improved cell detection in large animals.

Keywords

Single Photon Emission Compute Tomography Mesenchymal Stem Cell Middle Cerebral Artery Occlusion Prussian Blue Ischemic Lesion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Antje Y. Dreyer
    • 1
    • 2
  • Donald Lobsien
    • 3
  • Claudia Pösel
    • 1
  • Karl-Titus Hoffmann
    • 3
  • Björn Nitzsche
    • 1
  • Matthias Findeisen
    • 4
  • Albrecht Stroh
    • 5
  • Johannes Boltze
    • 1
    • 2
  1. 1.Department of Cell TherapyFraunhofer-Institute for Cell Therapy and ImmunologyLeipzigGermany
  2. 2.Translational Centre for Regenerative MedicineUniversity of LeipzigLeipzigGermany
  3. 3.Department for NeuroradiologyUniversity of LeipzigLeipzigGermany
  4. 4.Department of Chemistry and Mineralogy, Institute of Analytical ChemistryUniversity of LeipzigLeipzigGermany
  5. 5.Focus Group Translational Neurosciences (FTN), Institute for Microscopic Anatomy and NeurobiologyJohannes Gutenberg-University MainzMainzGermany

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