Kinetics of the Interaction of Peptidases with Substrates and Modifiers

  • Antonio BaiciEmail author
  • Marko Novinec
  • Brigita Lenarčič


This chapter is dedicated to reviewing, commenting and illustrating with examples kinetic tools that are essential to the study of peptidases interacting with their substrates, inhibitors and activators. Kinetic characterization starts with the measurement of the kinetic parameters for substrates using graphical and mathematical methods because this information is essential for further characterizing the action of modifiers. The bulk of the chapter is dedicated to the description of the properties and mechanisms of classical, tight binding and slow onset reversible inhibition, as well as to enzyme inactivation. The reader may notice that some beloved, ‘classical’ kinetic methods are not mentioned in this chapter. With all due respect for the past, we believe that modern methods, such as numerical integration of differential equations and robust statistical approaches, can reasonably replace less powerful investigation tools.


Progress Curve Reactive Center Loop Peptide Bond Hydrolysis Individual Rate Constant Active Site Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Antonio Baici
    • 1
    Email author
  • Marko Novinec
    • 2
  • Brigita Lenarčič
    • 2
  1. 1.Department of BiochemistryUniversity of ZurichZurichSwitzerland
  2. 2.Department of Chemistry and BiochemistryUniversity of LjubljanaLjubljanaSlovenia

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