Aquaporin-1 deletion reduces osmotic water permeability and cerebrospinal fluid production

  • K. Oshio
  • Y. Song
  • A. S. Verkman
  • Geoffrey T. ManleyEmail author
Conference paper
Part of the Acta Neurochirurgica Supplements book series (NEUROCHIRURGICA, volume 86)


Aquaporin-1 (AQP1) is a water channel that is strongly expressed at the ventricular-facing surface of choroid plexus epithelium. Using wildtype and AQP1 null mice, we developed novel methods to compare the water permeability in isolated choroid plexus, and cerebrospinal fluid (CSF) production in living mice. Osmoticallyinduced water transport was rapid in freshly isolated choroid plexus from wildtype mice as measured by a spatial-filtering optical method, and reduced by 5-fold by AQP1 deletion. CSF production, an isosmolar fluid secretion process, was measured by a dye dilution method involving fluid collections using a second microneedle introduced into the cisterna magna. CSF production in wildtype mice was (in μl/min) 0.37 ± 0.04 μl/min (control), 0.16 ± 0.03 μl/min (acetazolamide-treated) and 1.14 ± 0.15 μl/min (forskolin-treated), and reduced by up to 25% in AQP1 null mice. The impaired CSF production in AQP1 null mice provides direct functional evidence for the involvement of AQP1 in CSF formation.


Null Mouse Choroid Plexus Nephrogenic Diabetes Insipidus Wildtype Mouse Osmotic Water Permeability 
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Copyright information

© Springer-Verlag Wien 2003

Authors and Affiliations

  • K. Oshio
    • 1
  • Y. Song
    • 2
  • A. S. Verkman
    • 2
  • Geoffrey T. Manley
    • 1
    Email author
  1. 1.Department of Neurological SurgeryUniversity of CaliforniaSan FranciscoUSA
  2. 2.Department of MedicineUniversity of CaliforniaSan FranciscoUSA

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