Open-Channel Separation Techniques for the Characterization of Nanomaterials and Their Bioconjugates for Drug Delivery Applications

  • Jiwon Lee
  • Roxana Coreas
  • Wenwan ZhongEmail author


Open-channel separation techniques can separate samples without reliance on column packing, minimizing sample loss due to adsorption onto the packing materials and reducing damage to samples, in particular, the complexes held together by non-chemical interactions. Field flow fractionation (FFF) and capillary electrophoresis (CE) are two representative open-channel separation techniques. In this chapter, we discuss the use of FFF and CE to separate and characterize various nanomaterials widely applied in biomedical research.



The authors thank the support from the National Institute of Environmental Health Sciences of the National Institutes of Health under the Award #U01ES027293 (to W. Z.) and T32ES018827 (to R. C.).


  1. 1.
    Kim S, Lim YT, Soltesz EG, De Grand AM, Lee J, Nakayama A, Parker JA, Mihaljevic T, Laurence RG, Dor DM, Cohn LH, Bawendi MG, Frangioni JV (2004) Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping. Nat Biotechnol 22:93–97. Scholar
  2. 2.
    So M-K, Xu C, Loening AM, Gambhur SS, Rao J (2006) Self-illuminating quantum dots aid in vivo imaging. Nat Biotechnol 24:339–343. Scholar
  3. 3.
    Yu MK, Jeong YY, Park J, Park S, Kim JW, Min JJ, Kim K, Jon S (2008) Drug-loaded superparamagnetic iron oxide nanoparticles for combined cancer imaging and therapy in vivo. Angew Chemie – Int Ed 47:5362–5365. Scholar
  4. 4.
    Alexiou C, Arnold W, Klein RJ, Parak FG, Hulin P, Bergemann C, Erhardt W, Wagenpfeil S, Lübbe AS (2000) Locoregional cancer treatment with magnetic drug targeting. Cancer Res 60:6641–6648Google Scholar
  5. 5.
    Wang Y, Sun Y, Wang J, Yang Y, Li Y, Yuan Y, Liu C (2016) Charge-reversal APTES-modified mesoporous silica nanoparticles with high drug loading and release controllability. ACS Appl Mater Interfaces 8:17166–17175. Scholar
  6. 6.
    Zhang CY, Yeh HC, Kuroki MT, Wang TH (2005) Single-quantum-dot-based DNA nanosensor. Nat Mater 4:826–831. Scholar
  7. 7.
    Lundqvist M, Stigler J, Elia G, Lynch I, Cedervall T, Dawson KA (2008) Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts. Proc Natl Acad Sci 105:14265–14270. Scholar
  8. 8.
    Tenzer S, Docter D, Kuharev J, Musyanovych A, Fetz V, Hecht R, Schlenk F, Fischer D, Kiouptsi K, Reinhardt C, Landfester K, Schild H, Maskos M, Knauer SK, Stauber RH (2013) Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology. Nat Nanotechnol 8:772–781. Scholar
  9. 9.
    An H, Jin B (2012) Prospects of nanoparticle-DNA binding and its implications in medical biotechnology. Biotechnol Adv 30:1721–1732. Scholar
  10. 10.
    Villanueva A, Cañete M, Roca AG, Calero M, Veintemillas-Verdaguer S, Serna CJ, del Puerto Morales M, Miranda R (2009) The influence of surface functionalization on the enhanced internalization of magnetic nanoparticles in cancer cells. Nanotechnology 20:115103. Scholar
  11. 11.
    Westmeier D, Stauber RH, Docter D (2016) The concept of bio-corona in modulating the toxicity of engineered nanomaterials (ENM). Toxicol Appl Pharmacol 299:53–57. Scholar
  12. 12.
    Wang F, Yu L, Monopoli MP, Sandin P, Mahon E, Salvati A, Dawson KA (2013) The biomolecular corona is retained during nanoparticle uptake and protects the cells from the damage induced by cationic nanoparticles until degraded in the lysosomes. Nanomed Nanotechnol Biol Med 9:1159–1168. Scholar
  13. 13.
    Ke PC, Lin S, Parak WJ, Davis TP, Caruso F (2017) A decade of the protein corona. ACS Nano 11:11773CrossRefGoogle Scholar
  14. 14.
    Cedervall T, Lynch I, Lindman S, Berggard T, Thulin E, Nilsson H, Dawson KA, Linse S (2007) Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles. Proc Natl Acad Sci 104:2050–2055. Scholar
  15. 15.
    Ulbrich K, Holá K, Šubr V, Bakandritsos A, Tuček J, Zbořil R (2016) Targeted drug delivery with polymers and magnetic nanoparticles: covalent and noncovalent approaches, release control, and clinical studies. Chem Rev 116:5338–5431. Scholar
  16. 16.
    Filipe V, Hawe A, Jiskoot W (2010) Critical evaluation of nanoparticle tracking analysis (NTA) by NanoSight for the measurement of nanoparticles and protein aggregates. Pharm Res 27:796–810. Scholar
  17. 17.
    Wei GT, Liu F-K, Wang CRC (1999) Shape separation of nanometer gold particles by size-exclusion chromatography. Anal Chem 71:2085–2091. Scholar
  18. 18.
    Wilcoxon JP, Martin JE, Provencio P (2000) Size distributions of gold nanoclusters studied by liquid chromatography. Langmuir 16:9912–9920. Scholar
  19. 19.
    Hanauer M, Pierrat S, Zins I, Lotz A, Sönnichsen C (2007) Separation of nanoparticles by gel electrophoresis according to size and shape. Nano Lett 7:2881–2885. Scholar
  20. 20.
    Xu X, Caswell KK, Tucker E, Kabisatpathy S, Brodhacker KL, Scrivens WA (2007) Size and shape separation of gold nanoparticles with preparative gel electrophoresis. J Chromatogr A 1167:35–41. Scholar
  21. 21.
    Giddings JC (1966) A new separation concept based on a coupling of concentration and flow nonuniformities. Sep Sci 1:123–125. PublishedCrossRefGoogle Scholar
  22. 22.
    Giddings JC (1993) Field-flow fractionation – analysis of macromolecular, colloidal, and particulate materials. Science (80- ) 260:1456–1465. Scholar
  23. 23.
    Liu MK, Li P, Giddings JC (1993) Rapid protein separation and diffusion coefficient measurement by frit inlet flow field-flow fractionation. Protein Sci 2:1520–1531. Scholar
  24. 24.
    Giddings JC, Yang FJ, Myers MN (1977) Flow field-flow fractionation as a methodology for protein separation and characterization. Anal Biochem 81:395–407. Scholar
  25. 25.
    Liu MK, Giddings JC (1993) Separation and measurement of diffusion coefficients of linear and circular DNAs by flow field-flow fractionation. Macromolecules 26:3576–3588. Scholar
  26. 26.
    Ashby J, Schachermeyer S, Duan Y, Jimenez LA, Zhong W (2014) Probing and quantifying DNA-protein interactions with asymmetrical flow field-flow fractionation. J Chromatogr A 1358:217–224. Scholar
  27. 27.
    Bousse T, Shore DA, Goldsmith CS, Hossain MJ, Jang Y, Davis CT, Donis RO, Stevens J (2013) Quantitation of influenza virus using field flow fractionation and multi-angle light scattering for quantifying influenza A particles. J Virol Methods 193:589–596. Scholar
  28. 28.
    Giddings JC, Yang FJ, Myers MN (1977) Flow field-flow fractionation – new method for separating, purifying, and characterizing diffusivity of viruses. J Virol 21:131–138. Scholar
  29. 29.
    Flack K, Jimenez LA, Zhong W (2017) Analysis of the distribution profiles of circulating microRNAs by asymmetrical flow field flow fractionation. In: Rani S. (eds) MicroRNA profiling. Methods in molecular biology, vol 1509. Humana Press, New York, NY, pp 161–168Google Scholar
  30. 30.
    Wagner M, Pietsch C, Tauhardt L, Schallon A, Schubert US (2014) Characterization of cationic polymers by asymmetric flow field-flow fractionation and multi-angle light scattering-a comparison with traditional techniques. J Chromatogr A 1325:195–203. Scholar
  31. 31.
    Giddings JC (1973) The conceptual basis of field-flow fractionation. J Chem Educ 50:667. Scholar
  32. 32.
    Kowalkowski T, Buszewski B, Cantado C, Dondi F (2006) Field-flow fractionation: theory, techniques, applications and the challenges. Crit Rev Anal Chem 36:129–135. Scholar
  33. 33.
    Contado C (2017) Field flow fractionation techniques to explore the “nano-world”. Anal Bioanal Chem 409:2501–2518. Scholar
  34. 34.
    Bednar AJ, Poda AR, Mitrano DM, Kennedy AJ, Gray EP, Ranville JF, Hayes CA, Crocker FH, Steevens JA (2013) Comparison of on-line detectors for field flow fractionation analysis of nanomaterials. Talanta 104:140–148. Scholar
  35. 35.
    Schachermeyer S, Ashby J, Zhong W (2012) Advances in field-flow fractionation for the analysis of biomolecules: instrument design and hyphenation. Anal Bioanal Chem 404:1151–1158. Scholar
  36. 36.
    Szolar OHJ, Brown RS, Luong JHT (1995) Separation of PAHs by capillary electrophoresis with laser-induced fluorescence detection using mixtures of neutral and Anionic.beta.-cyclodextrins. Anal Chem 67:3004–3010. Scholar
  37. 37.
    Cheng HL, Liao YM, Chiou SS, Wu SW (2008) On-line stacking capillary electrophoresis for analysis of methotrexate and its eight metabolites in whole blood. Electrophoresis 29:3665–3673. Scholar
  38. 38.
    Sun L, Zhu G, Zhang Z, Mou S, Dovichi NJ (2015) Third-generation electrokinetically pumped sheath-flow nanospray interface with improved stability and sensitivity for automated capillary zone electrophoresis-mass spectrometry analysis of complex proteome digests. J Proteome Res 14:2312–2321. Scholar
  39. 39.
    Han F, Huynh BH, Ma Y, Lin B (1999) High-efficiency DNA separation by capillary electrophoresis in a polymer solution with ultralow viscosity. Anal Chem 71:2385–2389. Scholar
  40. 40.
    Nehme H, Nehme R, Lafite P, Routier S, Morin P (2012) New development in in-capillary electrophoresis techniques for kinetic and inhibition study of enzymes. Anal Chim Acta 722:127–135. Scholar
  41. 41.
    Mattarozzi M, Suman M, Cascio C, Calestani D, Weigel S, Undas A, Peters R (2017) Analytical approaches for the characterization and quantification of nanoparticles in food and beverages. Anal Bioanal Chem 409:63–80. Scholar
  42. 42.
    Bandyopadhyay S, Peralta-Videa JR, Gardea-Torresdey JL (2013) Advanced analytical techniques for the measurement of nanomaterials in complex samples: a comparison. Environ Eng Sci 30:118–125. Scholar
  43. 43.
    Sadik OA, Du N, Kariuki V, Okello V, Bushlyar V (2014) Current and emerging technologies for the characterization of nanomaterials. ACS Sustain Chem Eng 2:1707–1716. Scholar
  44. 44.
    Meermann B, Laborda F (2015) Analysis of nanomaterials by field-flow fractionation and single particle ICP-MS. J Anal At Spectrom 30:1226–1228. Scholar
  45. 45.
    Chetwynd A, Guggenheim E, Briffa S, Thorn J, Lynch I, Valsami-Jones E (2018) Current application of capillary electrophoresis in nanomaterial characterisation and its potential to characterise the protein and small molecule corona. Nanomaterials 8. Scholar
  46. 46.
    Yohannes G, Jussila M, Hartonen K, Riekkola ML (2011) Asymmetrical flow field-flow fractionation technique for separation and characterization of biopolymers and bioparticles. J Chromatogr A 1218:4104–4116. Scholar
  47. 47.
    Giddings JC, Yang FJF, Myers MN (1976) Flow field-flow fractionation: a versatile new separation method. Science (80- ) 193:1244–1245CrossRefGoogle Scholar
  48. 48.
    Mudalige TK, Qu H, Sánchez-Pomales G, Sisco PN, Linder SW (2015) Simple functionalization strategies for enhancing nanoparticle separation and recovery with asymmetric flow field flow fractionation. Anal Chem 87:1764–1772. Scholar
  49. 49.
    Rambaldi DC, Reschiglian P, Zattoni A (2011) Flow field-flow fractionation: recent trends in protein analysis. Anal Bioanal Chem 399:1439–1447. Scholar
  50. 50.
    Wahlund KG, Giddings JC (1987) Properties of an asymmetrical flow field-flow fractionation channel having one permeable wall. Anal Chem 59:1332–1339. Scholar
  51. 51.
    Mudalige TK, Qu H, Van Haute D, Ansar SM, Linder SW (2018) Capillary electrophoresis and asymmetric flow field-flow fractionation for size-based separation of engineered metallic nanoparticles: a critical comparative review. TrAC – Trends Anal Chem 106:202–212. Scholar
  52. 52.
    Schachermeyer S, Ashby J, Kwon M, Zhong W (2012) Impact of carrier fluid composition on recovery of nanoparticles and proteins in flow field flow fractionation. J Chromatogr A 1264:72–79. Scholar
  53. 53.
    Ashby J, Schachermeyer S, Pan S, Zhong W (2013) Dissociation-based screening of nanoparticle-protein interaction via flow field-flow fractionation. Anal Chem 85:7494–7501. Scholar
  54. 54.
    Ashby J, Flack K, Jimenez LA, Duan Y, Khatib AK, Somlo G, Wang SE, Cui X, Zhong W (2014) Distribution profiling of circulating MicroRNAs in serum. Anal Chem 86:9343–9349. Scholar
  55. 55.
    Chu YH, Avila LZ, Biebuyck HA, Whitesides GM (1992) Use of affinity capillary electrophoresis to measure binding constants of ligands to proteins. J Med Chem 35:2915–2917. Scholar
  56. 56.
    Chu Y-H, Avila LZ, Gao J, Whitesides GM (1995) Affinity capillary electrophoresis. Acc Chem Res 28:461–468. Scholar
  57. 57.
    Li N, Zeng S, He L, Zhong W (2010) Probing nanoparticle− protein interaction by capillary electrophoresis. Anal Chem 82:7460–7466CrossRefGoogle Scholar
  58. 58.
    Terabe S, Otsuka K, Ichikawa K, Tsuchiya A, Ando T (1984) Electrokinetic separations with micellar solutions and open-tubular capillaries. Anal Chem 56:111–113. Scholar
  59. 59.
    Liu FK, Wei GT (2004) Adding sodium dodecylsulfate to the running electrolyte enhances the separation of gold nanoparticles by capillary electrophoresis. Anal Chim Acta 510:77–83. Scholar
  60. 60.
    Ciriello R, Iallorenzi PT, Laurita A, Guerrieri A (2017) Improved separation and size characterization of gold nanoparticles through a novel capillary zone electrophoresis method using poly(sodium4-styrenesulfonate) as stabiliser and a stepwise field strength gradient. Electrophoresis 38:922–929. Scholar
  61. 61.
    Kairdolf BA, Qian X, Nie S (2017) Bioconjugated nanoparticles for biosensing, in vivo imaging, and medical diagnostics. Anal Chem 89:1015–1031. Scholar
  62. 62.
    Bazak R, Houri M, El Achy S, Kamel S, Refaat T (2015) Cancer active targeting by nanoparticles: a comprehensive review of literature. J Cancer Res Clin Oncol 141:769–784. Scholar
  63. 63.
    Hizir MS, Top M, Balcioglu M, Rana M, Robertson NM, Shen F, Sheng J, Yigit MV (2016) Multiplexed activity of perAuxidase: DNA-capped AuNPs act as adjustable peroxidase. Anal Chem 88:600–605. Scholar
  64. 64.
    Li H, Shen J, Cui R, Sun C, Zhao Y, Wu X, Li N, Tang B (2017) A highly selective and sensitive fluorescent nanosensor for dopamine based on formate bridged Tb(iii) complex and silver nanoparticles. Analyst 142:4240–4246. Scholar
  65. 65.
    Safenkova IV, Slutskaya ES, Panferov VG, Zherdev AV, Dzantiev BB (2016) Complex analysis of concentrated antibody-gold nanoparticle conjugates’ mixtures using asymmetric flow field-flow fractionation. J Chromatogr A 1477:56–63. Scholar
  66. 66.
    Poda AR, Bednar AJ, Kennedy AJ, Harmon A, Hull M, Mitrano DM, Ranville JF, Steevens J (2011) Characterization of silver nanoparticles using flow-field flow fractionation interfaced to inductively coupled plasma mass spectrometry. J Chromatogr A 1218:4219–4225. Scholar
  67. 67.
    Tadjiki S, Montaño MD, Assemi S, Barber A, Ranville J, Beckett R (2017) Measurement of the density of engineered silver nanoparticles using centrifugal FFF-TEM and single particle ICP-MS. Anal Chem 89:6056–6064. Scholar
  68. 68.
    Marassi V, Roda B, Casolari S, Ortelli S, Blosi M, Zattoni A, Costa AL, Reschiglian P (2018) Hollow-fiber flow field-flow fractionation and multi-angle light scattering as a new analytical solution for quality control in pharmaceutical nanotechnology. Microchem J 136:149–156. Scholar
  69. 69.
    Marassi V, Casolari S, Roda B, Zattoni A, Reschiglian P, Panzavolta S, Tofail SAM, Ortelli S, Delpivo C, Blosi M, Costa AL (2015) Hollow-fiber flow field-flow fractionation and multi-angle light scattering investigation of the size, shape and metal-release of silver nanoparticles in aqueous medium for nano-risk assessment. J Pharm Biomed Anal 106:92–99. Scholar
  70. 70.
    Wimuktiwan P, Shiowatana J, Siripinyanond A (2015) Investigation of silver nanoparticles and plasma protein association using flow field-flow fractionation coupled with inductively coupled plasma mass spectrometry (FlFFF-ICP-MS). J Anal At Spectrom 30:245–253. Scholar
  71. 71.
    Yu B, Zhou Y, Song M, Xue Y, Cai N, Luo X, Long S, Zhang H, Yu F (2016) Synthesis of selenium nanoparticles with mesoporous silica drug-carrier shell for programmed responsive tumor targeted synergistic therapy. RSC Adv 6:2171–2175. Scholar
  72. 72.
    Zheng S, Li X, Zhang Y, Xie Q, Wong YS, Zheng W, Chen T (2012) PEG-nanolized ultrasmall selenium nanoparticles overcome drug resistance in hepatocellular carcinoma HepG2 cells through induction of mitochondria dysfunction. Int J Nanomedicine 7:3939–3949. Scholar
  73. 73.
    M-M P, Somchue W, Shiowatana J, Siripinyanond A (2014) Flow field-flow fractionation for particle size characterization of selenium nanoparticles incubated in gastrointestinal conditions. Food Res Int 57:208–209. Scholar
  74. 74.
    Seabra A, Durán N (2015) Nanotoxicology of metal oxide nanoparticles. Metals (Basel) 5:934–975. Scholar
  75. 75.
    Peng N, Wu B, Wang L, He W, Ai Z, Zhang X, Wang Y, Fan L, Ye Q (2016) High drug loading and pH-responsive targeted nanocarriers from alginate-modified SPIONs for anti-tumor chemotherapy. Biomater Sci 4:1802–1813. Scholar
  76. 76.
    Martínez-Carmona M, Gun’ko Y, Vallet-Regí M (2018) ZnO nanostructures for drug delivery and theranostic applications. Nanomaterials 8. Scholar
  77. 77.
    Bogdan J, Plawinska-Czarnak J, Zarzynska J (2017) Nanoparticles of titanium and zinc oxides as novel agents in tumor treatment: a review Janusz. Nanoscale Res Lett 12:225. Scholar
  78. 78.
    Ashby J, Pan S, Zhong W (2014) Size and surface functionalization of iron oxide nanoparticles influence the composition and dynamic nature of their protein corona. ACS Appl Mater Interfaces 6:15412–15419. Scholar
  79. 79.
    Weber C, Simon J, Mailänder V, Morsbach S, Landfester K (2018) Preservation of the soft protein corona in distinct flow allows identification of weakly bound proteins. Acta Biomater 76:217–224. Scholar
  80. 80.
    Wang S, McGuirk CM, d’Aquino A, Mason JA, Mirkin CA (2018) Metal-organic framework nanoparticles. Adv Mater 30:1800202. Scholar
  81. 81.
    Roda B, Marassi V, Zattoni A, Borghi F, Anand R, Agostoni V, Gref R, Reschiglian P, Monti S (2018) Flow field-flow fractionation and multi-angle light scattering as a powerful tool for the characterization and stability evaluation of drug-loaded metal–organic framework nanoparticles. Anal Bioanal Chem 410:5245–5253. Scholar
  82. 82.
    Hinna AH, Hupfeld S, Kuntsche J, Brandl M (2016) The use of asymmetrical flow field-flow fractionation with on-line detection in the study of drug retention within liposomal nanocarriers and drug transfer kinetics. J Pharm Biomed Anal 124:157–163. Scholar
  83. 83.
    Elgqvist J, Frost S, Pouget J-P, Albertsson P (2014) The potential and hurdles of targeted alpha therapy – clinical trials and beyond. Front Oncol 3:1–9. Scholar
  84. 84.
    Huclier-Markai S, Grivaud-Le Du A, N’tsiba E, Montavon G, Mougin-Degraef M, Barbet J (2018) Coupling a gamma-ray detector with asymmetrical flow field flow fractionation (AF4): application to a drug-delivery system for alpha-therapy. J Chromatogr A 1573:107–114. Scholar
  85. 85.
    Moquin A, Neibert KD, Maysinger D, Winnik FM (2015) Quantum dot agglomerates in biological media and their characterization by asymmetrical flow field-flow fractionation. Eur J Pharm Biopharm 89:290–299. Scholar
  86. 86.
    Bouzas-Ramos D, García-Cortes M, Sanz-Medel A, Encinar JR, Costa-Fernández JM (2017) Assessment of the removal of side nanoparticulated populations generated during one-pot synthesis by asymmetric flow field-flow fractionation coupled to elemental mass spectrometry. J Chromatogr A 1519:156–161. Scholar
  87. 87.
    Menéndez-Miranda M, Encinar JR, Costa-Fernández JM, Sanz-Medel A (2015) Asymmetric flow field-flow fractionation coupled to inductively coupled plasma mass spectrometry for the quantification of quantum dots bioconjugation efficiency. J Chromatogr A 1422:247–252. Scholar
  88. 88.
    Matczuk M, Anecka K, Scaletti F, Messori L, Keppler BK, Timerbaev AR, Jarosz M (2015) Speciation of metal-based nanomaterials in human serum characterized by capillary electrophoresis coupled to ICP-MS: a case study of gold nanoparticles. Metallomics 7:1364–1370. Scholar
  89. 89.
    Belder D, Deege A, Husmann H, Kohler F, Ludwig M (2001) Cross-linked poly(vinyl alcohol) as permanent hydrophilic column coating for capillary electrophoresis. Electrophoresis 22:3813–3818.<3813::AID-ELPS3813>3.0.CO;2-DCrossRefGoogle Scholar
  90. 90.
    Matczuk M, Legat J, Shtykov SN, Jarosz M, Timerbaev AR (2016) Characterization of the protein corona of gold nanoparticles by an advanced treatment of CE-ICP-MS data. Electrophoresis 37:2257–2259. Scholar
  91. 91.
    Legat J, Matczuk M, Scaletti F, Messori L, Timerbaev A, Jarosz M (2017) Erratum to: CE separation and ICP-MS detection of gold nanoparticles and their protein conjugates. Chromatographia 80:1719. Scholar
  92. 92.
    Man Y, Lv X, Iqbal J, Jia F, Xiao P, Hasan M, Li Q, Dai R, Geng L, Qing H, Deng Y (2013) Adsorptive BSA coating method for CE to separate basic proteins. Chromatographia 76:59–65. Scholar
  93. 93.
    Boulos SP, Davis TA, Yang JA, Lohse SE, Alkilany AM, Holland LA, Murphy CJ (2013) Nanoparticle-protein interactions: a thermodynamic and kinetic study of the adsorption of bovine serum albumin to gold nanoparticle surfaces. Langmuir 29:14984–14996. Scholar
  94. 94.
    Gao J, Huang X, Liu H, Zan F, Ren J (2012) Colloidal stability of gold nanoparticles modified with thiol compounds: bioconjugation and application in cancer cell imaging. Langmuir 28:4464–4471. Scholar
  95. 95.
    López-Lorente ÁI, Soriano ML, Valcárcel M (2014) Analysis of citrate-capped gold and silver nanoparticles by thiol ligand exchange capillary electrophoresis. Microchim Acta 181:1789–1796. Scholar
  96. 96.
    Pakiari AH, Jamshidi Z (2010) Nature and strength of M-S bonds (M = Au, Ag, and Cu) in binary alloy gold clusters. J Phys Chem A 114:9212–9221CrossRefGoogle Scholar
  97. 97.
    Gautier J, Munnier E, Soucé M, Chourpa I, Douziech Eyrolles L (2015) Analysis of doxorubicin distribution in MCF-7 cells treated with drug-loaded nanoparticles by combination of two fluorescence-based techniques, confocal spectral imaging and capillary electrophoresis. Anal Bioanal Chem 407:3425–3435. Scholar
  98. 98.
    Blazkova I, Nguyen HV, Dostalova S, Kopel P, Stanisavljevic M, Vaculovicova M, Stiborova M, Eckschlager T, Kizek R, Adam V (2013) Apoferritin modified magnetic particles as doxorubicin carriers for anticancer drug delivery. Int J Mol Sci 14:13391–13402. Scholar
  99. 99.
    Oukacine F, Bernard S, Bobe I, Cottet H (2014) Physico-chemical characterization of polymeric micelles loaded with platinum derivatives by capillary electrophoresis and related methods. J Control Release 196:139–145. Scholar
  100. 100.
    Musile G, Cenci L, Andreetto E, Ambrosi E, Tagliaro F, Bossi AM (2016) Screening of the binding properties of molecularly imprinted nanoparticles via capillary electrophoresis. Anal Bioanal Chem 408:3435–3443. Scholar
  101. 101.
    Taylor G (1953) Dispersion of soluble matter in solvent flowing slowly through a tube. Proc R Soc A Math Phys Eng Sci 219:186–203. Scholar
  102. 102.
    Ibrahim A, Meyrueix R, Pouliquen G, Chan YP, Cottet H (2013) Size and charge characterization of polymeric drug delivery systems by Taylor dispersion analysis and capillary electrophoresis. Anal Bioanal Chem 405:5369–5379. Scholar
  103. 103.
    Franzen U, Østergaard J (2012) Physico-chemical characterization of liposomes and drug substance-liposome interactions in pharmaceutics using capillary electrophoresis and electrokinetic chromatography. J Chromatogr A 1267:32–44. Scholar
  104. 104.
    Nguyen TTTN, Østergaard J, Stürup S, Gammelgaard B (2013) Metallomics in drug development: characterization of a liposomal cisplatin drug formulation in human plasma by CE-ICP-MS. Anal Bioanal Chem 405:1845–1854. Scholar
  105. 105.
    Nguyen TTTN, Østergaard J, Stürup S, Gammelgaard B (2013) Determination of platinum drug release and liposome stability in human plasma by CE-ICP-MS. Int J Pharm 449:95–102. Scholar
  106. 106.
    Otarola J, Lista AG, Fernández Band B, Garrido M (2015) Capillary electrophoresis to determine entrapment efficiency of a nanostructured lipid carrier loaded with piroxicam. J Pharm Anal 5:70–73. Scholar
  107. 107.
    Janu L, Stanisavljevic M, Krizkova S, Sobrova P, Vaculovicova M, Kizek R, Adam V (2013) Electrophoretic study of peptide-mediated quantum dot-human immunoglobulin bioconjugation. Electrophoresis 34:2725–2732. Scholar
  108. 108.
    Zhou ZM, Feng Z, Zhou J, Fang BY, Ma ZY, Liu B, Zhao YD, Hu XB (2015) Quantum dot-modified aptamer probe for chemiluminescence detection of carcino-embryonic antigen using capillary electrophoresis. Sensors Actuators B Chem 210:158–164. Scholar

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Authors and Affiliations

  1. 1.Department of ChemistryUniversity of California-RiversideRiversideUSA
  2. 2.Environmental Toxicology Graduate ProgramUniversity of California-RiversideRiversideUSA

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