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A Modular Framework for Post-Processing and Analysis of Fluorescence Microscopy Image Sequences of Subcellular Calcium Dynamics

  • Daniel Schetelig
  • Insa M.A. Wolf
  • Björn-P. Diercks
  • Ralf Fliegert
  • Andreas H. Guse
  • Alexander Schlaefer
  • Rene Werner
Conference paper
Part of the Informatik aktuell book series (INFORMAT)

Abstract

Calcium (Ca2+) signaling is essential for activation of Tlymphocytes and can be understood as fundamental on-switch for the adaptive immune system. The activation is supposed to start by initial spatially and temporally localized Ca2+ signals. Imaging and analysis of these signals require high spatio-temporal resolution fluorescence microscopy – which, in turn, results in the need for an efficient and reliable post-processing and analysis workflow of the acquired image data. Started with a well established but time-consuming post-processing process, we report on our efforts to automatize and optimize it. The efforts led to a modular post-processing and analysis framework, which is presented. In addition, the influence of instances of the main blocks of the framework (e.g. bleaching correction, deconvolution) on Ca2+ dynamics analysis measures is evaluated.

Keywords

Main Block Deconvolution Algorithm Modular Framework Deconvolution Approach Acquire Image Data 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Daniel Schetelig
    • 1
  • Insa M.A. Wolf
    • 2
  • Björn-P. Diercks
    • 2
  • Ralf Fliegert
    • 2
  • Andreas H. Guse
    • 2
  • Alexander Schlaefer
    • 3
  • Rene Werner
    • 1
  1. 1.Department of Computational NeuroscienceHamburg-HarburgDeutschland
  2. 2.Department of Biochemistry and Molecular Cell BiologyUniversity Medical Center Hamburg-EppendorfHamburg-EppendorfDeutschland
  3. 3.Institute of Medical TechnologyHamburg University of TechnologyHamburgDeutschland

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