Continuous Magnetic Extraction for Protein Purification

Chapter
First Online:
Part of the Lecture Notes in Bioengineering book series (LNBE)

Abstract

Continuous Magnetic Extraction (CME) is a process for the separation and purification of proteins from crude feedstocks. Magnetic particles with functional surface groups, e.g., ion exchange ligands, serve as carriers for the target protein. Together with a mixture of thermoresponsive surfactants and binding buffer these carriers are added to the feed containing the target protein. After binding the target within minutes, the mixture is heated above the LCST of the surfactant and phase separation is induced. Through selective partitioning of the carrier particles to the top phase and partitioning of contaminants to the bottom phase of the system, purification is possible. The proof-of-concept of this bioseparation process has been recently released, and new devices for CME have been developed within the framework of the EU project MagPro2Life and the respective results are reported in this chapter. Several particle types and feed streams, representing a broad field of potential applications, were tested with regard to their suitability for the CME process.

Keywords

Lower Critical Solution Temperature Feed Stream Bottom Phase Surfactant Rich Phase Separation Chamber 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AMTPS

Aqueous Micellar Two-Phase Systems

ATPS

Aqueous Two-Phase Systems

CME

Continuous Magnetic Extraction

DEAP

Diethylaminopropyl groups

LCST

Lower Critical Solution Temperature

MEP

Magnetic Extraction Phases

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Institute of Functional Interfaces (IFG)Karlsruhe Institute of TechnologyEggenstein-LeopoldshafenGermany

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