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Role of Acid-Base Disturbance on Potassium Transport Along the Nephron

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Nephrology

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Summary

We evaluated the role of acidosis on the regulation of transepithelial potassium transport in rabbit early proximal convoluted tubules (PCT) and cortical collecting ducts (CCD) by using in vitro microperfusion and conventional microelectrode methods. In PCT, when the bath medium pH declined from 7.4 to 6.8, transepithelial voltage (Vt) and net potassium flux (JK) increased; however, in CCD, Vt and JK decreased significantly without changing net Na flux. In CCD, basolateral acidosis decreased basolateral membrane voltage and increased transepithelial resistance, with an increment of calculated fractional resistance of apical membrane in principal cells. Inhibition of JK by basolateral acidosis remained significant in the presence of 2mM luminal BaC12. Elimination of ambient bicarbonate (Hepes buffer solution) did not affect the inhibitory effect of basolateral acidosis on JK. Basolateral 1 mM amiloride diminished the inhibitory effect of basolateral acidosis on JK. The 86Rb and 22Na efflux coefficients were not significantly affected by basolateral acidosis.

In conclusion, the present study demonstrates that basolateral acidosis affects JK in both PCT and CCD, but it does so in opposite directions. In CCD, basolateral pH is indeed an important modulator of epithelial K transport. Mechanistically, basolateral acidosis appears to inhibit apical K conductance independently of Na conductance or ambient bicarbonate in rabbit CCD.

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Authors and Affiliations

  1. Div. of Nephrology, Dept. of Medicine, Jichi Medical School, 3311-1 Minamikawachi-machi, Kawachi-gun, Tochigi, 329-04, Japan

    Kaoru Tabei, Shigeaki Muto, Hiroaki Furuya, Yasunori Sakairi, Yasuhiro Ando & Yasushi Asano

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  1. Kaoru Tabei
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  2. Shigeaki Muto
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  3. Hiroaki Furuya
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  4. Yasunori Sakairi
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  5. Yasuhiro Ando
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  6. Yasushi Asano
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Editors and Affiliations

  1. Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan

    Michinobu Hatano M.D. (Professor of Medicine, Director) (Professor of Medicine, Director)

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© 1991 Springer Japan

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Tabei, K., Muto, S., Furuya, H., Sakairi, Y., Ando, Y., Asano, Y. (1991). Role of Acid-Base Disturbance on Potassium Transport Along the Nephron. In: Hatano, M. (eds) Nephrology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-35158-1_19

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  • DOI: https://doi.org/10.1007/978-3-662-35158-1_19

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-70074-6

  • Online ISBN: 978-3-662-35158-1

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