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The Effects of Negative Stains on Lipids and Proteins Observed in the Electron Microscope

  • R. W. Horne
Chapter

Abstract

Negative staining techniques have been applied to a wide variety of biological structures, including bacteria, viruses, sub-cellular components, proteins and lipids (cf. Horne 1965). Phospholipids in the form of lecithin as well as mixtures of lecithin and cholesterol have been studied at the molecular level by B a n g h a m and Horne (1964) and Lucy and Glauert (1964). Moreover, modifications of the phospholipid biomolecular leaflet by various chemical agents were also observed.

References

  1. B a n g h a m, A. D., and R. W. Horn e, 1964: J. Molec. Biol. 8, 660.Google Scholar
  2. Grant, R. A., R. W. Horne, and R. W. Cox, 1963: Nature 207, 822.CrossRefGoogle Scholar
  3. Horn e, R. W., 1965: In: “Techniques for Electron Microscopy” (Ed. D. K a y). 2nd Edition. Blackwells, Oxford, p. 328.Google Scholar
  4. Lucy, J. A., and A. M. GI a u e r t, 1964: J. Molec. Biol. 8, 727.Google Scholar
  5. Roth field, L., and R. W. Ho r n e: (To be published.)Google Scholar

Copyright information

© Springer-Verlag Wien 1967

Authors and Affiliations

  • R. W. Horne
    • 1
  1. 1.Agricultural Research CouncilInstitute of Animal PhysiologyCambridgeEngland

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