Wheat Anther Culture Using Liquid Media
Chapter
Abstract
The production of doubled haploid lines from anther culture is limited by the relatively low callus/embryoid induction frequency, genotype-dependent response, and poor plant regeneration, a large number of which are albino plants. As a result, this technique has yet to gain wide acceptance by wheat breeders, although it is being used by a few. This chapter will review the progress and advantages offered by liquid culture media in wheat.
Keywords
Winter Wheat Induction Medium Anther Culture Doubled Haploid Line Concentration Of90
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References
- Araki C (1956) Structure of agarose constituent of agar-agar. Bull Chem Soc Jpn 29:543–544 Armstrong TA, Metz SG, Mascia PN (1987) Two regeneration systems for the production of haploid plants from wheat anther culture. Plant Sci 51: 231–237Google Scholar
- Datta SK, Wenzel G (1987) Isolated microspore derived plant formation via embryogenesis in Triticum aestivum L. Plant Sci 48: 49–54CrossRefGoogle Scholar
- Deaton WR, Metz SG, Armstrong TA, Mascia PN (1987) Genetic analysis of the anther culture-response of three spring wheat crosses. Theor Appl Genet 74: 334–338CrossRefGoogle Scholar
- Debergh PD (1983) Effects of agar brand and concentration on the tissue culture medium. Physiol Plant 59: 270–276CrossRefGoogle Scholar
- Heberle-Bors E, Odenbach W (1985) In vitro pollen embryogenesis and cytoplasmic male sterility in Triticum aestivum L. Z Pflanzenzucht 95: 14–22Google Scholar
- Henry Y, de Buyser J (1981) Float culture of wheat anthers. Theor Appl Genet 60: 77–79CrossRefGoogle Scholar
- Henry Y, de Buyser J, Guenegou T, Ory C (1984) Wheat microspore embryogenesis during in vitro anther culture. Theor Appl Genet 67: 439–442CrossRefGoogle Scholar
- Kao KN (1981) Plant formation from barley anther cultures with Ficoll media. Z Pflanzenphysiol 103: 437–443Google Scholar
- Kelly SL (1986) The effect of sucrose, dextran and glucose on anther culture of winter wheat (Triticum aestivum L.). M Sci Thesis, Univ Guelph, 103 ppGoogle Scholar
- Kohlenbach HW, Wernicke W (1978) Investigations on the inhibitory effect of agar and the function of active carbon in anther culture. Z Pflanzenphysiol 86: 463–472Google Scholar
- Lazar MD, Schaeffer GW, Baenziger PS (1985) The physical environment in relation to high frequency callus and plantlet development in anther cultures of wheat (Triticum aestivum L.) cv. Chris. J Plant Physiol 121: 103–109Google Scholar
- Lettre JJ (1986) Effects of anther orientation and medium composition on in vitro culture of wheat (Triticum aestivum L.) anthers. M Sci Thesis, Univ Guelph, 108 ppGoogle Scholar
- Lyne RL, Bennett RI, Hunter CP (1984) Embryoid and plant production from cultured barley anthers. In: Withers LA, Alderson PG (eds) Plant tissue culture and its agricultural applications. Butterworth, London Guildford, pp 405–411Google Scholar
- Marsolais AA (1985) Callus induction from barley microspores. Ph D Thesis, Univ Guelph, 124 pp Marsolais AA, Kasha KJ (1985) Callus induction from barley microspores 1. The role of sucrose and auxin in a barley anther culture medium. Can J Bot 63: 2209–2212Google Scholar
- Marsolais AA, Wheatley WA, Kasha KJ (1986) Progress in wheat and barley haploid induction using anther culture. In: Proc Plant Breed Symp, Lincoln, NZ, DSIR, pp 340–343Google Scholar
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
- Olsen FL (1987) Induction of microspore embryogenesis in cultured anthers of Hordeum vulgare. The effects of ammonium nitrate, glutamine and asparagine as nitrogen sources. Carlsberg Res Commun 52: 393–404CrossRefGoogle Scholar
- Schaeffer GW, Baenziger PS, Worley J (1979) Haploid plant development from anthers and in vitro embryo culture of wheat. Crop Sci 19: 667–702CrossRefGoogle Scholar
- Shillito RD, Paszkowski J, Potrykus I (1983) Agarose plating and bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species. Plant Cell Rep 2: 294–247CrossRefGoogle Scholar
- Thiebaut J, Kasha KJ, Tsai A (1979) Influence of plant development stage, temperature, and plant hormones on chromosome doubling of barley haploids using colchicine. Can J Bot 57: 480–483CrossRefGoogle Scholar
- Wei ZM (1982) Pollen callus culture in Triticum aestivum. Theor Appl Genet 63: 71–73CrossRefGoogle Scholar
- Ye JM, Kao KN, Harvey BL, Rossnagel BG (1987) Screening salt tolerant barley genotypes via anther culture in high salt media. Theor Appl Genet 74: 426–429CrossRefGoogle Scholar
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