Cellulase and Amylase Complexes

  • A. Radford
  • P. J. Stone
  • F. Taleb
Chapter
Part of the The Mycota book series (MYCOTA, volume 3)

Abstract

Fungi with cellulolytic activity have a major role in recycling cellulose, the world’s most abundant biological molecule. This biodegradation occurs in the rotting process of all plant material. In this process, the cellulose macromolecule, a β-1,4-linked glucose polymer, usually found in close association with lignin, is converted into soluble glucose monomers from an insoluble polymer by a complex of three major classes of activity: (i) endoglucanase, (ii) exoglucanase (cellobiohydrolase) and (iii) β-glucosidase.

Keywords

Aspergillus Niger Catalytic Domain Hinge Region Trichoderma Reesei Neurospora Crassa 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Abdullah M, Fleming ID, Taylor PM, Whelan WJ (1963) Substrate specificity of the amyloglucosidases of Aspergillus niger. Biochem J 89, 35 pGoogle Scholar
  2. Abuja PM, Schmuck M, Pilz I, Claeyssens T, Esterbauer H (1988) Structural and functional domains in cellobiohydrolase I from Trichoderma reesei. EurBiophys J 15: 339 - 342Google Scholar
  3. Aleshin A, Golubev A, Firsov LM, Honzatko RB (1992) Crystal structure of glucoamylase from Aspergillus awamori var. X100 to 2.2 A resolution. J Biol Chem 267: 19291 - 19298PubMedGoogle Scholar
  4. Allen JD, Thoma JA (1976) Subsite mapping of enzymes: application of the depolymerase computer model to two a-amylases. Biochem J 159: 121 - 132PubMedGoogle Scholar
  5. Ashikari T, Nakamura N, Tanaka Y, Kiuchi N, Shihano Y, Tanaka T, Amachi T, Yosizumi H (1986) Rhizopus rawstarch-degrading glucoamylase–its cloning and expression in yeast. Agric Biol Chem 50: 957 - 964Google Scholar
  6. Azevedo M d’O, Felipe MSS, Astolfi-Filho S, Radford A (1990) Cloning, sequencing and homology of the cbh-I (exoglucanase) gene of Humicola grisea var. thermoidea. J Gen Microbiol 136: 2569 - 2576Google Scholar
  7. Beguin P (1990) Molecular biology of cellulose degradation. Ann Rev Microbiol 44: 219 - 248CrossRefGoogle Scholar
  8. Boel E, Hjort I, Svensson B, Norris F. Fiil NP (1984) Glucoamylase-G1 and glucoamylase-G2 from Aspergillus niger are synthesised from two different but closely related messenger RNAs. EMBO J 3: 1097 - 1102Google Scholar
  9. Boel E, Brady L, Brzozowski AM, Derewenda Z, Dodson GG, Jensen VJ, Petersen SB. Swift H, Thim L, Woldike HF (1990) Calcium binding in alpha-amylases: an X-ray diffraction study at 2.1 A resolution of two enzymes from Aspergillus. Biochemistry 29:6244-6249Google Scholar
  10. Chang M (1971) Folding chain model and annealing of cellulose. J Polymer Sci 36: 343 - 362Google Scholar
  11. Chen CM, Gritzali M, Stafford DW (1987) Nucleotide sequence and deduced primary structure of cellobiohydrolase II from Trichoderma reesei. Bio/Technology 5: 274 - 278CrossRefGoogle Scholar
  12. Chen LJ, Ford C, Nikolov Z (1991) Adsorption to starch of a ß-galactosidase fusion protein containing the starch-binding region of Aspergillus glucoamylase. Gene 99: 121 - 126PubMedCrossRefGoogle Scholar
  13. Cheng C, Tsukagoshi N, Udaka S (1990) Nucleotide sequence of the cellobiohydrolase gene from Trichoderma viride. Nucl Acids Res 18: 5559 - 5559PubMedCrossRefGoogle Scholar
  14. Clarke AJ, Svensson B (1984) The role of tryptophanyl residues in the function of Aspergillus niger glucoamylase-GI and glucoamylase-G2. Carlsberg Res Commun 49: 559 - 566CrossRefGoogle Scholar
  15. Claros MG, del Pozo L, Abarca D, Jimenez A (1992) The promoter element GTACAAG of the SGA and STA2 genes is a possible target site for repression by the STAIO gene product from Saccharomyces cerevisiae. FEMS Microbiol Lett 92: 57 - 62CrossRefGoogle Scholar
  16. Coughlan MP (1992) Enzymatic hydrolysis of cellulose: an overview. Bioresource Technol 39: 107 - 115CrossRefGoogle Scholar
  17. Covert SF, Bolduc J, Cullen D (1992) Genomic organisation of a cellulase gene family in Phanerochaete chrysosporium. Curr Genet 22: 407 - 413PubMedCrossRefGoogle Scholar
  18. Dohmen JR, Strasser AW, Dahlems UM. Hollenberg CP (1990) Cloning the Schwanniornyces occidentalis glucoamylase gene ( GAMI) and its expression in Saccharomyces cerevisiae. Gene 95: 111-121Google Scholar
  19. El Gogary S, Leitz A, Crivellaro O, Eveleigh DE, El Doury H (1989) Mechanism by which cellulose triggers cellobiohydrolase I gene expression in Trichoderma reesei. Proc Natl Acad Sci USA 86: 6138 - 6141PubMedCrossRefGoogle Scholar
  20. Eriksson KE, Hamp SG (1978) Regulation of endo-1, 4-ßD-glucanase production in Sporotrichum pulverulentum. Eur J Biochem 90: 183 - 190PubMedCrossRefGoogle Scholar
  21. Fogarty WM (1983) Microbial amylases. In: Fogarty WM (ed) Microbial enzymes and biotechnology. Applied Science Publishers, London, pp 1 - 92Google Scholar
  22. Fogarty WM, Benson CP (1983) Purification and properties of a thermophilic amyloglycosidase from Aspergillus niger. Eur J Appl Microbiol Biotech 18: 271 - 278CrossRefGoogle Scholar
  23. Fowler T, Berka R, Ward M (1990) Regulation of the glaA gene of Aspergillus niger. Curr Genet 18: 537 - 545PubMedCrossRefGoogle Scholar
  24. Fujii M, Kawamura Y (1985) Synergistic action of a-amylase and glucoamylase on hydrolysis of starch. Biotechnol Bioeng 27: 260 - 265PubMedCrossRefGoogle Scholar
  25. Fujii M, Homma T, Taniguchi M (1988) Synergism of a-amylase and glucoamylase on hydrolysis of native starch granules. Biotechnol Bioeng 32: 910 - 915PubMedCrossRefGoogle Scholar
  26. Gilligan W, Reese ET (1954) Evidence for multiple components in microbial cellulases. Can J Microbiol 1: 90 - 107PubMedCrossRefGoogle Scholar
  27. Gong CS, Tsao GT (1979) Cellulase and biosynthesis regu- lation. Ann Rep Ferment Proc 3: 111 - 140Google Scholar
  28. Hata Y, Kitamoto K. Gomi K, Kumagai C, Tamura G (1991) Functional elements of the Aspergillus oryzae glaA gene encoding glucoamylase. Curr Genet 22: 85 - 91Google Scholar
  29. Hata Y, Kitamoto K, Gomi K, Kumagai C, Tamura G (1992) Functional elements of the promoter region of the Aspergillus orvzae glaA gene encoding glucoamylase. Curr Genet 22: 85 - 91PubMedCrossRefGoogle Scholar
  30. Hayashida S, Nomura T, Yoshino E, Hongo M (1975) The formation and properties of subtilisin-modified glucoamylase. Agric Biol Chem 40: 141 - 146CrossRefGoogle Scholar
  31. Hayashida S, Kuroda K, Ohta K, Kuhara S, Fukuda K, Sakaki Y (1989) Molecular cloning of the glucomnylaseI gene of Aspergillus awanrori var. kawachi for localization of the raw-starch-affinity site. Agric Biol Chem 53: 923 - 929CrossRefGoogle Scholar
  32. Henrissat B, Claessens M, Tomme P, Lemesle L, Mornon JP (1989) Cellulase families revealed by hydrophobic cluster analysis. Gene 81: 83 - 89PubMedCrossRefGoogle Scholar
  33. Hiromi K, Kawai M, Ono S (1966) Kinetic studies on glut-amylase–IV: hydrolysis of isomaltose. J Biochem 59: 476 - 480PubMedGoogle Scholar
  34. Hostinova E, Balanova J, Gasperik J (1991) The nucleotide sequence of the glucoamylase gene glal from Saccharomycopsis fibuligera KZ. FEMS Microbiol Lett 83: 103 - 108CrossRefGoogle Scholar
  35. Itoh T, Ohtsuki I, Yamashita I, Fukui S (1987) Nucleotide sequence of the glucoamylase gene GLUT in the yeast Saccharomycopsis fibuligera. J Bacteriol 169: 41714176Google Scholar
  36. Jackson MA, Talburt DE (1988) Purification and partial characterisation of an extracellular ß-glucosidase of Trichoderma reesei using cathodic run, polyacrylamide-gel electrophoresis. Biotechnol Bioeng 32: 903909Google Scholar
  37. Jespersen HM, Macgregor EA, Sierks MR, Svensson B (1991) Comparison of domain-level organisation of starch hydrolases and related enzymes. Biochem J 280: 51 - 55PubMedGoogle Scholar
  38. Knowles J, Lehtovaara P, Teeri T (1987) Cellulase families and their genes. Trends Biotechnol 5: 255 - 260CrossRefGoogle Scholar
  39. Kubicek CP, Eveleigh DE, Esterbauer H, Steiner W, Pranz EM (eds) (1990) Trichoderma reesei cellulases: biochemistry, genetics, physiology and applications. Proc TRICEL 89 Meet, R Soc Chem. LondonGoogle Scholar
  40. MacGregor EA, Svensson B (1989) A super-secondary structure predicted to be common to several alpha1,4-o-glucan-cleaving enzymes. Biochem J 259: 145 - 152PubMedGoogle Scholar
  41. Matsuura Y, Kusunoki M, Harada W, Kakudo M (1984) Structure and possible catalytic residues of Takaamylase A. J Biochem 95: 697 - 702PubMedGoogle Scholar
  42. Neustroev KN, Golubev AM, Firsov LM, Ibatullin FM, Protasevich II, Makarov AA (1993) Effect of modification of carbohydrate component on properties of glucoamylase. FEBS Lett 316: 157 - 160PubMedCrossRefGoogle Scholar
  43. Nunberg JH, Meade JH, Cole G, Lawyer FC, McCabe P, Schweickart V, Tal R, Wittman VP, Flatgaard JE, Innis MA (1984) Molecular cloning and characterisation of the glucoamylase gene of Aspergillus awanrori. Mol Cell Biol 4: 2306 - 2315PubMedGoogle Scholar
  44. Ooi T, Shinmyo A, Okada H, Hara S, Ikenaka T, Murao S, Arai M (1990) Cloning and sequence analysis of a cDNA for cellulase (FI -CMCase) from Aspergillus aculeatus. Curr Genet 18: 217 - 222PubMedCrossRefGoogle Scholar
  45. Pardo JM, Ianez E, Zalacain M, Claros MG, Jimenez A (1988) Similar short elements in the 5’ regions of the ST-A2 and SGA genes from Saccharomyces cerevisiae. FEBS Lett 239: 179 - 184PubMedCrossRefGoogle Scholar
  46. Penttila M, Lehtovaara P, Nevalainen H, Bhikhabhai R, Knowles J (1986) Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endonuclease I gene. Gene 45: 253 - 263PubMedCrossRefGoogle Scholar
  47. Quiocho FA (1986) Carbohydrate-binding proteins: tertiary structures and protein-sugar interactions. Annu Rev Biochem 55: 287 - 315PubMedCrossRefGoogle Scholar
  48. Saloheimo M, Lehtovaara P, Penttila M, Teeri T, Stahlbeg J, Johansson J, Pettersson G, Claeyssens M, Tomme P, Knowles J (1988) EGIII, a new endoglucanase from Trichoderma reesei: the characterisation of both gene and enzyme. Gene 63: 11 - 21PubMedCrossRefGoogle Scholar
  49. Shibuya I, Gomi K, Iimura Y, Takahashi K, Tamura G, Hara S (1990) Molecular cloning of the glucoamylase gene of Aspergillus shirousami and its expression in Aspergillus oryzae. Agric Biol Chem 54: 1905 - 1914PubMedCrossRefGoogle Scholar
  50. Shima H, Inui M, Akada R, Yamashita I (1989) Upstream regions of the yeast glucoamylase gene which are required for efficient transcription. Agric Biol Chem 53: 749 - 755CrossRefGoogle Scholar
  51. Shoemaker S, Schweikart V, Ladner M, Gelfand D, Kwok S, Myambo K, Innis M (1983) Molecular cloning of exo-cellobiohydrolase I derived from Trichoderma reesei strain L27. Bio/Technology 1: 691 - 696CrossRefGoogle Scholar
  52. Sierks MR, Svensson B (1992) Kinetic identification of a hydrogen-bonding pair in the glucoamylase-maltose transition-state complex. Protein Eng 5: 185 - 188PubMedCrossRefGoogle Scholar
  53. Sierks MR, Svensson B (1993) Functional roles of the invariant aspartic acid 55, tyrosine 306, and aspartic acid 309 in glucoamylase from Aspergillus awamori studied by mutagenesis. Biochemistry 32: 1113 - 1117PubMedCrossRefGoogle Scholar
  54. Sierks MR, Ford C, Reilly PJ, Svensson B (1990) Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of ASP176, GLU179 and GLU180 in the enzyme from Aspergillus awamori. Protein Eng 3: 193 - 198PubMedCrossRefGoogle Scholar
  55. Sierks MR, Ford C, Reilly PJ, Svensson B (1993) Functional roles and subsite locations of LEU177, TRP178 and ASNI82 of Aspergillus awamori glucoamylase determined by site-directed mutagenesis. Protein Eng 6: 75 - 79PubMedCrossRefGoogle Scholar
  56. Sims P, James C, Broda P (1988) The identification, molecular cloning and characterisation of a gene from Phanerochaete chrysosporium that shows strong homology to the exocellobio-hydrolase of Trichoderma reesei. Gene 74: 411 - 422PubMedCrossRefGoogle Scholar
  57. Stahlberg J, Johansson G, Pettersson G (1988) A bindingsite-deficient, catalytically active core protein of endoglucanase-III from the culture filtrate of Trichoderma reesei. Eur J Biochem 173: 179 - 183PubMedCrossRefGoogle Scholar
  58. Stone PJ, Makoff AJ, Parish JH, Radford A (1993) Cloning and sequence analysis of the glucoamylase gene of Neurospora crassa. Curr Genet 24: 205 - 211PubMedCrossRefGoogle Scholar
  59. Strasser AWM, Selk R, Dohmen RJ, Niermann T, Bielefeld M, Seeboth P, Tu G, Hollenberg CP (1989) Analysis of the alpha-amylase gene of Schwanniomyces occidentalis and the secretion of its gene product in transformants of different yeast genera. Eur J Biochem 184: 699 - 706PubMedCrossRefGoogle Scholar
  60. Svensson B, Sierks MR (1992) Roles of the aromatic side-chains in the binding of substrates, inhibitors, and cyclomaltooligo-saccharides to the glucoamylase from Aspergillus niger probed by perturbation difference spectroscopy, chemical modification, and mutagenesis. Carbohydr Res 227: 29 - 47PubMedCrossRefGoogle Scholar
  61. Svensson B, Clarke AJ, Svendsen I (1986a) Influence of acarbose and maltose on the reactivity of individual tryptophanyl residues in glucoamylase from Aspergillus niger. Carlsberg Res Commun 51: 61 - 73CrossRefGoogle Scholar
  62. Svensson B, Larsen K, Gunuarrson A (1986b) Characterisation of a glucoamylase G2 from Aspergillus niger. Eur J Biochem 154: 497 - 502PubMedCrossRefGoogle Scholar
  63. Svensson B, Jespersen H, Sierks MR, Macgregor EA (1989) Sequence homology between putative raw-starch binding domains from different starch-degrading enzymes. Biochem J 264: 309 - 311PubMedGoogle Scholar
  64. Svensson B, Clarke AJ, Svendsen I, Moller H (1990) Identification of carboxylic acid residues in glucoamylase-G2 from Aspergillus niger that participate in catalysis and substrate binding. Eur J Biochem 188: 29 - 38PubMedCrossRefGoogle Scholar
  65. Tada S, Imura Y, Gomi K, Takahashi K, Hara S, Yoshizawa K (1989) Cloning and nucleotide sequence of the genomic Taka-amylase A gene of Aspergillus oryzae. Agric Biol Chem 53: 593 - 599CrossRefGoogle Scholar
  66. Tada S, Gomi K, Kitamoto K, Kumagai C, Tamuta G, Hara S (1991) Identification of the promoter region of the Taka-amylase A gene required for starch induction. Agric Biol Chem 55: 1939 - 1941PubMedCrossRefGoogle Scholar
  67. Taleb F, Radford A (1995) The cellulase complex of Neurospora coassa: cbh-1 cloning: sequencing and homologies. Gene 161: 137 - 138PubMedCrossRefGoogle Scholar
  68. Teeri T, Salovuori I, Knowles J (1 983) The molecular cloning of the major cellulase gene from Trichoderma reesei. Bio/Technology 1: 696 - 699Google Scholar
  69. Teeri T, Lehtovaara P, Kauppinen S, Salovuori I, Knowles J (1987) Homologous domains in Trichoderma reesei cellulase enzymes: gene sequence and expression of CBHII. Gene 51: 43 - 52PubMedCrossRefGoogle Scholar
  70. Toda H, Kondo K, Narita K (1982) The complete amino acid sequence of Taka-amylase A. Proc Jpn Acad 58B: 208 - 212Google Scholar
  71. Tonomura K, Suzuki H, Nakamura N, Kuraya K, Tanabe O (1961) On the inducers of a-amylase formation in Aspergillus oryzae. Agric Biol Chem 25: 1 - 10Google Scholar
  72. Williamson G, Belshaw NJ, Noel TR, Ring SG, Williamson MP (1992a) O-glycosylation and stability - unfolding of glucoamylase induced by heat and guanidine hydrochloride. Eur J Biochem 207: 661 - 670PubMedCrossRefGoogle Scholar
  73. Williamson G, Belshaw NJ, Williamson MP (1992b) Oglycosylation in Aspergillus glucoamylase - conformation and role in binding. Biochem J 282: 423 - 428PubMedGoogle Scholar
  74. Wirsel S, Lachmund A, Wildhardt G, Ruttkowski E (1989) Three alpha-amylase genes of Aspergillus oryzae exhibit identical intron-exon organisation. Mol Microbiol 3: 3 - 14PubMedCrossRefGoogle Scholar
  75. Wood M (1975) Properties of cellulolytic enzyme systems. Biochem Soc Trans 13: 407 - 410Google Scholar
  76. Woodward J (1991) Synergism in cellulase systems. Bioresource Technol 36: 67 - 75CrossRefGoogle Scholar
  77. Yamashita I, Suzuki K, Fukui S (1985) Nucleotide sequence of the extraacellular glucoamylase gene STAZ in the yeast Saccharomyces cerevisiae. J Bacteriol 161: 567573Google Scholar
  78. Yamashita I, Nakamura M, Fukui S (1987) Gene fusion is a possible mechanism underlying the evolution of STAZ. J Bacteriol 169: 2142 - 2149PubMedGoogle Scholar
  79. Yazdi MT, Woodward JR, Radford A (1990a) Cellulase production by Neurospora crassa: the enzymes of the complex and their regulation. Enzyme Microb Technol 12: 116 - 119CrossRefGoogle Scholar
  80. Yazdi MT, Radford A, Keen JR, Woodward JR (1990b) Cellulose production by Neurospora crassa: purification and characterisation of cellulolytic enzymes. Enzyme Microb Technol 12: 120 - 123PubMedCrossRefGoogle Scholar
  81. Yazdi MT, Woodward JR, Radford A (1990e) The cellulase complex of Neurospora crassa: activity, stability and release. J Gen Microbiol 136: 1313 - 1319PubMedGoogle Scholar
  82. Yoshino E, Hayashida S (1978) Formation of active derivatives of glucoamylase I during the digestion with fungal acid protease and a-mannosidase. Agric Biol Chem 42: 927 - 933CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1996

Authors and Affiliations

  • A. Radford
    • 1
  • P. J. Stone
    • 2
  • F. Taleb
    • 1
  1. 1.Department of GeneticsThe University of LeedsLeedsUK
  2. 2.Department of Biochemistry and Molecular BiologyThe University of LeedsLeedsUK

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