Mating-Type Cassettes: Structure, Switching and Silencing
From a single haploid P (plus) or M (minus) cell, S. pombe is able to produce a population of haploids containing both mating types in nearly equal proportions. This ability is widespread among fungi, the presence of both mating types permitting the formation of diploids and eventually spores. Single-cell lineage approaches have revealed the asymmetry and rules of mating-type switching in S. pombe. Genetic and biochemical analyses have identified the organization of the mating-type loci and a number of genes required for the process of switching. They have yielded insights into the molecular mechanism of the switch and into two processes of epigenetic inheritance. The first process exploits the intrinsic asymmetry of DNA synthesis to restrain a gene conversion event to one of two sister chromatids. The second allows the formation and maintenance of a silent chromatin state. The interplay of both epigenetic events provides a striking example of dynamic chromatin choreography allowing progression into the S. pombe developmental program.
KeywordsGene Conversion Fission Yeast Schizosaccharomyces Pombe Gene Conversion Event Switching Potential
Unable to display preview. Download preview PDF.
- Bjerling P (1998) Positional silencing in the mating-type region of Schizosaccharomyces pombe. PhD Thesis, University of CopenhagenGoogle Scholar
- Borgstrom B (1995) Characterization of the Schizosaccharomyces pombe rikl gene. PhD thesis, University of CopenhagenGoogle Scholar
- Klar AJ (1992) Molecular genetics of fission yeast cell type: mating type and mating-type interconversion. In: Jones EW, Pringle JR, Broach JR (eds) The molecular and cellular biology of the yeast Saccharomyces - gene expression. CSH Laboratory Press, Cold Spring Harbor, pp 745–777Google Scholar
- Leupold U (1950) Die Vererbung von Homothallie und Heterothallie bei Schizosaccharomyces pombe. CR Lab Carlsberg Ser Physiol. 24: 381–480Google Scholar
- Leupold U (1955) Methodisches zur Genetik von Schizosaccharomyces pombe. Schweiz Z Allg Pathol Bakteriol 18: 161–170Google Scholar
- Rudolph C, Fleck 0, Kohli J (1998) Schizosaccharomyces pombe exol is involved in the same mismatch repair pathway as msh2 and pmsl. Curr Genet 34: 343–350Google Scholar
- Schmidt H, Kapitza P, Gutz H (1987) Switching genes in Schizosaccharomyces pombe: Their influence on cell viability and recombination. Curr Genet 11: 303–308Google Scholar
- Styrkarsdottir U, Egel R, Nielsen 0 (1993) The smt-0 mutation which abolishes mating-type switching in fission yeast is a deletion. Curr Genet 23: 184–186Google Scholar
- Tornier C, Bessone S, Varlet I et al. (2001) Requirement for Msh6, but not for Swi4 (Msh3), in Msh2-dependent repair of base-base mismatches and mononucleotide loops in Schizo-saccharomyces pombe. Genetics 158: 65–75Google Scholar