Effects of Pressure on the Growth Kinetics of Orthorhombic Lysozyme Crystals

  • Y. Suzuki
  • H. Suto
  • T. Sawada
  • K. Tamura
Conference paper

Abstract

Solubility and growth rate of orthorhombic lysozyme (from hen egg-white) crystals were measured at 0.1 and 100 MPa. A growth or dissolution rate of a given concentration was measured, and the solubility was determined as the concentration at which the crystal did not grow or dissolve. The solubility decreased with increasing pressure at 30, 35 and 40 °C. This means that the supersaturation, σ (= lnC/C e, C: protein concentration, C e: solubility), increases; hence, the crystallization is enhanced with increasing pressure. The growth rates of (010) face, R(010), were replotted with σ, and the kinetic growth coefficients, k (= R(010) /σ), were estimated. The coefficient increased with increasing pressure at 30, 35 and 40 °C. This means that the growth kinetics is also enhanced_with increasing pressure.

Keywords

Dissolution Rate Growth Kinetic Partial Molar Volume Silicone Tube Seed Crystal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • Y. Suzuki
    • 1
  • H. Suto
    • 1
  • T. Sawada
    • 2
  • K. Tamura
    • 1
  1. 1.Department of Chemical Science and Technology, Faculty of EngineeringThe University of TokushimaTokushimaJapan
  2. 2.National Institute for Materials ScienceTsukubaJapan

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