Influence of High Pressure on the Secondary Structure of Poly-L-Lysine

  • H. Plangger
  • M. Scheibenzuber
  • G. Blümelhuber
  • R. Meyer-Pittroff
Conference paper

Abstract

In this study the influence of high-pressure treatment on the secondary structure conformation of Poly-L-Lysine was studied by circular dichroism measurement after treatment with pressures up to 600 MPa and various dwell times. Before HP treatment PLL was transformed into certain secondary structures by setting pH and temperature. Detected alterations in secondary structure indicate dependency on pressure altitude and dwell time.

Keywords

Secondary Structure Circular Dichroism Dwell Time Protein Secondary Structure Random Structure 
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References

  1. [1]
    Davidson B, Fasman GD (1967). The Conformational Transitions of Uncharged PolyL-Lysine. Biochemistry 6 1616–1629.CrossRefGoogle Scholar
  2. [2]
    Provencher SW, Glöckner J (1981). Estimation of globular protein secondary structure from circular dichroism. Biochemistry 20 33–37.CrossRefGoogle Scholar
  3. [3]
    Sreerama N, Venyaminov SY, Woody RW (2000). Estimation of Protein Secondary Structure from Circular Dichroism Spectra: Inclusion of Denatured Proteins with Native Proteins in the Analysis. Analytical Biochemistry 287 243–251.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • H. Plangger
    • 1
  • M. Scheibenzuber
    • 1
  • G. Blümelhuber
    • 1
  • R. Meyer-Pittroff
    • 1
  1. 1.Weihenstephan Center of Life and Food Sciences, Chair of Energy and Environmental Technologies of the Food IndustryTechnical University MunichFreisingGermany

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