Selectable and Screenable Markers for Rice Transformation

  • R. M. Twyman
  • E. Stöger
  • A. Kohli
  • T. Capell
  • P. Christou
Chapter
Part of the Molecular Methods of Plant Analysis book series (MOLMETHPLANT, volume 22)

Abstract

Rice transformation is a major goal in cereal biotechnology, not only because rice is the world’s most important food crop but also because this species has now been recognized as the model for cereal genomics. A number of alternative transformation strategies are available, the most widely used of which are particle bombardment (Christou et al. 1991) and Agrobacterium-mediated transformation (Hiei et al. 1994). Regardless of the chosen strategy, transformation is a low-efficiency process. Since most foreign genes introduced into plants do not confer a phenotype that can be used conveniently for the identification or selective propagation of transformed cells, marker genes must therefore be introduced along with the transgene of interest to provide such a phenotype.

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© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • R. M. Twyman
  • E. Stöger
  • A. Kohli
  • T. Capell
  • P. Christou

There are no affiliations available

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