Bacterial Wilt Disease pp 103-109 | Cite as
Molecular Methods for Detection and Discrimination of Ralstonia solanacearum
Chapter
Abstract
In the past decade a large range of nucleic acid and serological detection and rimination tests for R. solanacearum have been developed. These tests offer disc either increased sensitivity, specificity, speed or discrimination over previous techniques. The aim of this paper is to discuss the relevant advantages and applicabilitybilit of these tests, and their suitability to laboratories in less-developed countries.
Keywords
Ralstonia Solanacearum Solanacearum Strain Remote Laboratory Bacterial Wilt Disease Blood Disease Bacterium
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
- Cook D, Barlow E, Sequeira L (1989) Genetic diversity of Pseudomonas solanacearum: detection of restriction fragment length polymorphisms that specify virulence and the hypersensitive response. Mol Plant-Microbe Inter 2:113 – 121CrossRefGoogle Scholar
- Eden-Green S, Seal S (1993) Bacterial Diseases Of Banana And Plantain In South East Asia. Pages 115 – 121 In: Breeding Banana and Plantain for Resistance to Diseases and Pests (ed. Ganry J.) Montpellier, France: CIRAD in collaboration with INIBAPGoogle Scholar
- Engelbrecht MC (1994) Modification of a semi-selective medium for the isolation and quantification of Pseudomonas solanacearum. ACIAR Bact Wilt Newsl 10: 3 – 5Google Scholar
- Gillings M, Fahy P (1993) Genomic fingerprinting: towards a unified view of the Pseudomonas solanacearum complex. In: Hartman GL, Hayward AC (eds) Bacterial Wilt, Proceedings of an international symposium, Kaohsiung, Taiwan, ROC, 28 – 30 October 1992. ACIAR Proceedings 45, 85 – 92Google Scholar
- Holloway B, St G Bowen A, Escuadra M (1993) Whole genome analysis of Pseudomonas. In: Hartman GL, Hayward AC (eds) Bacterial Wilt, Proceedings of an international symposium, Kaohsiung, Taiwan, ROC, 28 – 30 October 1992. ACIAR Proceedings 45, 22 – 27Google Scholar
- Louws FJ, Fulbright DW, Taylor Stephens C, De Bruijn FJ (1994) Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Appl Environ Microbiol 60:2286 – 2295PubMedGoogle Scholar
- Seal S, Elphinstone J (1994) Advances in detection and identification of P. solanacearum. In: Bacterial Wilt. AC Hayward, GL Hartman (eds) CAB International, Wallingford, Oxon, UK., 35 – 57Google Scholar
- Seal SE, Jackson LA, Young JPW, Daniels MJ (1993) Differentiation of P. solanacearum, P. syzygii, P. pickettii and the blood disease bacterium by partial 16S rRNA sequencing: construction of oligonucleotide primers for sensitive detection by polymerase chain reaction. J Gen Microbiol 139:1587 – 1594PubMedCrossRefGoogle Scholar
- Seal S, Jackson L, Daniels M (1992) The use of tRNA consensus primers to indicate subgroups of Pseudomonas solanacearum by polymerase chain reaction amplification. Appl Environ Microbiol 58:3759 – 3761PubMedGoogle Scholar
- Seal S, Taghavi M, Fegan N, Hayward A, Fegan M (submitted) PCR tests for determination of Ralstonia solanacearum (synonym Pseudomonas solanacearum) rDNA subgroup.Google Scholar
- Skoglund L, Seal S, Elphinstone J, Berrios D (1993) Study of latent infection of potato tubers by Pseudomonas solanacearum in Burundi. In: Hartman GL, Hayward AC (eds) Bacterial Wilt Proceedings of an international symposium, Kaohsiung, Taiwan, ROC, 28 – 30 October, 1992. ACIAR Proceedings 45, 106 – 110Google Scholar
- Smith JJ, Offord LC, Holderness M, Saddler GS (1995) Genetic diversity of Burkholderia solanacearum (synonym Pseudomonas solanacearum) race 3 in Kenya. Appl Environ Microbiol 61:4263 – 4268PubMedGoogle Scholar
- Taghavi M, Hayward C, Sly Li, Fegan M (1996) Analysis of the phylogenetic relationships of strains of Burkholderia solanacearum, Pseudomonas syzygii and the blood disease bacterium based on sequencing of the-16S rRNA gene sequences. Int J Syst Bacteriol 46:10 – 15PubMedCrossRefGoogle Scholar
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