Genetic Transformation of Somatic Embryos

  • D. Ellis
Chapter
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 30)

Abstract

Although DNA can be introduced into virtually any plant cell, several biological constraints still exist for the regeneration of transformed plants. These biological constraints are linked to the ability of an individual cell to respond to both the introduced DNA and to a regeneration stimulus. For transformation success, the plant cell must have certain attributes which include (1) the competence to express the introduced DNA; (2) the competence for stable integration of introduced DNA; and (3) the competence to regenerate into a whole plant. The competence to express introduced DNA at a high level as well as ability to differentiate plants from single cells has made somatic embryogenesis a useful tool in plant transformation. In fact, somatic embryogenesis has been instrumental in the regeneration of transformed plants from recalcitrant species in such divergent plant groups as monocots and gymnosperms. Examples of somatic embryogenesis used in the regeneration of transformed plants are listed in Table 1.

Keywords

Somatic Embryo Somatic Embryogenesis Embryogenic Callus Zygotic Embryo Particle Bombardment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • D. Ellis
    • 1
  1. 1.Department of HorticultureUniversity of WisconsinMadisonUSA

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