Homologous Radioimmunoassay for Human Parathyroid Hormone (Residues 53–84) with Tyrosylated Peptide as Tracer

  • W. Hitzler
Chapter

Abstract

Measurement of carboxyl-terminal fragments of human parathyroid hormone (hPTH) helps to distinguish patients with primary hyperparathyroidism from healthy controls [12, 22]. In renal failure, the greatly increased concentration of circulating carboxyl-terminal fragments results from hypersecretion of the hormone by the parathyroid glands and its inadequate removal from the circulation [14]. The main disadvantage of existing carboxyl-terminal PTH assays is that sometimes the results for healthy individuals, patients with tumor hypercalcemia, and patients with proven primary hyperparathyroidism overlap [2]. This overlapping could be related to (e.g.) differences in the structure of bovine, porcine, and human hormone, resulting in incomplete recognition of the structure of the human hormone by the antibody. Therefore we have tried to develop a largely homologous radioimmunoassay (RIA) system for measurement of PTH in human serum, based on the use of a commercially available synthetic fragment of human PTH (residues 53–84).

Keywords

Parathyroid Hormone Primary Hyperparathyroidism Parathyroid Adenoma Human Parathyroid Hormone Sequential Saturation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Arnaud CD, Goldsmith RS, Bordier PJ, Sizemore GW (1974) Influence of immunoheterogeneity of circulating parathyroid hormone on results of radioimmunoassays of serum in man. Am J Med 56:785–793PubMedCrossRefGoogle Scholar
  2. 2.
    Ashby JP, Thakkar H (1988) Diagnostic limitations of region-specific parathyroid hormone assays in the investigation of hypercalcemia. Ann Clin Biochem 25:275–279PubMedGoogle Scholar
  3. 3.
    Bouillon R, Koninckx P, De Moor P (1974) A radioimmunoassay for human serum parathyroid hormone: methods and clinical evaluation. In: Radioimmunoassay and related procedures in medicine. International Atomic Energy Agency, Vienna, pp 353–365Google Scholar
  4. 4.
    Boyd JC, Lewis JW, Slatopolsky E, Ladenson JH (1981) Parathyrin measured concurrently with free or total calcium in the differential diagnosis of hypercalcemia. Clin Chem 27:574–579PubMedGoogle Scholar
  5. 5.
    Desplan C, Jullienne A, Moukhtar MS, Milhaud G (1977) Sensitive assay for biologically active fragment of human parathyroid hormone. Lancet ii:198–199CrossRefGoogle Scholar
  6. 6.
    Di Bella FP, Kehrwald JM, Laakso K, Zitzner L (1978) Parathyrin radioimmunoassay: diagnostic utility of antisera produced against carboxyl-terminal fragments of the hormone from the human. Clin Chem 24:451–454PubMedGoogle Scholar
  7. 7.
    Di Bella FP, Gilkinson JB, Flueck J, Arnaud CD (1978) Carboxyl-terminal fragments of human parathyroid hormone in parathyroid tumors: unique new source of immunogens for the production of antisera potentially useful in the radioimmunoassay of parathyroid hormone in human serum. J Clin Endocrinol Metab 46:604–612PubMedCrossRefGoogle Scholar
  8. 8.
    Di Bella FP, Hawker CD, McCann DS, Martin KJ, Heath H III, Anast CS, Forman DT (1982) Parathyrin (parathyroid hormone): radioimmunoassay for intact and carboxyl-terminal moieties. Clin Chem 28:226–235PubMedGoogle Scholar
  9. 9.
    Gorog RH, Hakim MK, Thompson NW, Rigg GA, McCann DS (1982) Radioimmunoassay of serum parathyrin: comparison of five commercial kits. Clin Chem 28:87–91PubMedGoogle Scholar
  10. 10.
    Hunter WM, Greenwood FC (1962) Preparation of iodine-131-labelled human growth hormone of high specific activity. Nature 194:495–496PubMedCrossRefGoogle Scholar
  11. 11.
    Kyle LH, Canary JJ, Mintz DH, De Leon A (1962) Inhibitory effects of induced hypercalcemia on secretion of parathyroid hormone. J Clin Endocrinol Metab 22:52–58CrossRefGoogle Scholar
  12. 12.
    Mallette LE (1980) Sensitivity of the antibovine parathyroid hormone serum 211/32 to synthetic fragments of human parathyroid hormone. J Clin Endocrinol Metab 50:201–203PubMedCrossRefGoogle Scholar
  13. 13.
    Niall HD, Sauer RT, Jacobs JW, Keutmann HT, Segre GV, O’Riordan JLH, Aurbach GD, Potts JT Jr (1974) The amino-acid sequence of the amino-terminal 37 residues of human parathyroid hormone. Proc Natl Acad Sci USA 71:384–388PubMedCrossRefGoogle Scholar
  14. 14.
    Papapoulos SE, Hendy GN, Tomlinson S, Lewin IG, O’Riordan JLH (1977) Clearance of exogenous parathyroid hormone in normal and uremic man. Clin Endocrinol 7:211–225CrossRefGoogle Scholar
  15. 15.
    Schöneshöfer M, Kage A, Kage R, Fenner A (1982) A convenient technique for the specific isolation of 125I-labelled peptide molecules. Fresenius Z Anal Chem 311:429–430CrossRefGoogle Scholar
  16. 16.
    Sofroniew MV, Madler M, Müller OA, Scriba PC (1978) A method for the consistent production of high quality antisera to small peptide hormones. Fresenius Z Anal Chem 290:163CrossRefGoogle Scholar
  17. 17.
    Solling H (1981) A rapid and inexpensive radioimmunoassay for parathyroid hormone utilizing the Wide-principle in combination with an improved separation techniq ue. Clin Chim Acta 116:349–359PubMedCrossRefGoogle Scholar
  18. 18.
    Thorell JI, Johansson BG (1971) Enzymic iodination of polypeptides with iodine-125 to high specific activity. Biochim Biophys Acta 251:363–369PubMedCrossRefGoogle Scholar
  19. 19.
    Voll R, Schmidt-Gayk H, Wiedemann J, Hüfner M, Bouillon R, Keutmann Hehrmann R (1978) Radioimmunoassay for parathyrin. Characterization of six different antigens and antisera. J Clin Chem Clin Biochem 16:269–277PubMedGoogle Scholar
  20. 20.
    Wood WG (1983) A comparison of six commercial kits for the determination of parathyrin (PTH) levels in serum. Ärztl Lab 29:311–316Google Scholar
  21. 21.
    Wood WG, Butz R, Casaretto M, Hehrmann R, Jüppner H, Marschner I, Wachter C, Zahn H, Hesch RD (1980) Preliminary results on the use of an antiserum to human parathyrin in a homologous radioimmunoassay. J Clin Chem Clin Biochem 18:789–795PubMedGoogle Scholar
  22. 22.
    Zillikens D, Armbruster FP, Stern J, Schmidt-Gayk H, Raue F (1987) Sensitive homologous radioimmunoassay for human parathyroid hormone to diagnose hypoparathyroid conditions. Ann Clin Biochem 24:608–613PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • W. Hitzler

There are no affiliations available

Personalised recommendations