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Liquid-Overlay Culture of Cellular Spheroids

  • J. Carlsson
  • J. M. Yuhas
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 95)

Abstract

The aim of this work has been to develop realistic and easily handled models of tumor growth, from which increased knowledge of the physical and chemical factors that govern or sometimes disturb cell proliferation may be obtained. Emphasis has been laid mainly on the properties of cultured spheroids originating from mammalian cells. The three-dimensional arrangement affects the concentration of oxygen and other substances and invokes, for cells inside the spheriods, physical, chemical, and nutritional stress factors that are probably typical of poorly vascularized areas of solid tumors. Increased knowledge of this situation is important, because it is believed to determine radiation sensivity and also the effects of cytotoxic substances.

Keywords

Human Glioma Multicellular Spheroid Multicellular Tumour Spheroid Catabolic Product Spheroid Surface 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Acker H, Carlsson J, Stalnacke CG (1983) Electrophysiological measurements in cultured cellular spheroids. Acta Pathol Microbiol Scand [A] 91: 151–160Google Scholar
  2. Ackerman LW, Rosai J (1974) Surgical pathology. Mosby, St LouisGoogle Scholar
  3. Allison DC, Yuhas JM, Ridolpho PF, Anderson SL, Johnson TS (1983) Cytophotometric measurement of the cellular DNA content of (3H)thymidine-labelled spheroids: Demonstration that some non-labelled cells have S and G2 DNA content. Cell Tissue Kinet 16: 237–246PubMedGoogle Scholar
  4. Andrews JR (1978) The radiobiology of human cancer radiotherapy. University Park Press, Baltimore MDGoogle Scholar
  5. Brunk U (1973) Lyosomes and residual bodies in neurons and in vitro cultivated glia cells. Acta Univ Upsal 148Google Scholar
  6. Brunk U, Ericsson I (1972) The demonstration of acid phosphatase in vitro cultured cells. Studies on the significance of fixation, tonicity and permeability. Histochem J 4: 349–363Google Scholar
  7. Carlsson J (1977) Proliferation gradient in three-dimensional colonies of cultured human glioma cells. Int J Cancer 20: 129–136PubMedCrossRefGoogle Scholar
  8. Carlsson J (1978) Tumor models in vitro. A study of poliferation and growth in cellular spheroids. Acta Univ Upsal 466Google Scholar
  9. Carlsson J, Brunk U (1977) The fine structure of three-dimensional colonies of human glioma cells in agarose culture. Acta Pathol Microbiol Scand [A] 85: 183–192Google Scholar
  10. Carlsson J, Collins P, Brunk U (1978) Plasma membrane motility and proliferation of human glioma cells in agarose and monolayer cultures. Acta Pathol Microbiol Scand [A] 86: 45–55Google Scholar
  11. Carlsson J, Nilsson K, Westermark B, Ponten J, Sundstrom C, Larsson E, Berg J, Palman S, Bush C, Collins P (1983) Formation and growth of multicellular spheroids of human origin. Int J Cancer 31: 523–533PubMedCrossRefGoogle Scholar
  12. Carlsson J, Nederman T (1983) A method to measure the radio and chemosensitivity of human spheroids. Adv Exp Med Biol 159: 399–417PubMedGoogle Scholar
  13. Carlsson J, Stalnacke CG, Acker H, Haji-Karim M, Nilsson S, Larsson B (1979) The influence of oxygen on viability and proliferation in cellular spheroids. Int J Radiat Oncol Biol Phys 5: 2011–2020PubMedCrossRefGoogle Scholar
  14. Charbit A, Malaise EP, Tubiana M (1971) Relation between the pathological nature and growth rate of human tumours. Eur J Cancer 7: 307–315PubMedCrossRefGoogle Scholar
  15. Cooper EH (1973) The biology of cell death in tumours. Cell Tissue Kinet 6: 87–95PubMedGoogle Scholar
  16. Costachel O, Fadel L, Badea E (1969) Tumour cell suspension culture on non-adhesive substratum. Z Krebsforsch 72: 24–31PubMedCrossRefGoogle Scholar
  17. Cox R, Masson WK (1974) Cloning of human diploid cells. Int J Radiat Biol 26: 193–198CrossRefGoogle Scholar
  18. Dalen H, Burki HJ (1971) Some observations on the three-dimensional growth of L5178Y cell colonies in soft agar culture. Exp Cell Res 65: 433–439PubMedCrossRefGoogle Scholar
  19. Denekamp J, Kallman RF (1973) In vitro and in vivo labelling of animal tumours with tritiated thymidine. Cell Tissue Kinet 6: 217–228PubMedGoogle Scholar
  20. Durand RE (1975) Cure, regression and cell survival: A comparison of common radiobiological endpoints using an in vitro tumour model. Br J Cancer 48: 556–571Google Scholar
  21. Durand RE (1980) Variable radiobiological responses of spheroids. Radiat Res 81: 85–89PubMedCrossRefGoogle Scholar
  22. Durand RE, Olive PL (1976) Irradiation of multi-cell spheroids with fast neutrons versus X-rays; a qualitative difference in sub-lethal damage repair capacity or kinetics. Int J Rad Biol 30: 589–592CrossRefGoogle Scholar
  23. Durand RE, Sutherland RM (1973) Growth and radiation survival characteristics of V79 — 171-b chinese hamster cells: A possible influence of intercellular contact. Radiat Res 56: 513–527Google Scholar
  24. Folkman J (1975) Tumor angiogenesis. In: Becker F (ed) Cellular biology and growth. New York, pp 355–388 (Cancer, vol 3 )Google Scholar
  25. Friedman M (1974) Paradoxes of the law of Bergonie and Tribondeau. In: Friedman M (ed) The biological and clinical basis of radiosensitivity. Thomas, Springfield, pp 389–390Google Scholar
  26. Gray LH (1961) Radiobiology basis of oxygen as a modifying factor in radiation therapy. AJR 85: 803–815Google Scholar
  27. Gullino PM (1975) Extracellular compartments of solid tumours. In: Becker F (ed) Cellular biology and growth. Plenum, New York, pp 327–354 (Cancer, vol 3 )Google Scholar
  28. Haji-Karim M, Carlsson J (1978) Proliferation and viability in cellular spheroids of human origin. Cancer Res 38: 1457–1464PubMedGoogle Scholar
  29. Hall EJ (1978) Radiobiology for the radiologist. Harper amp; Row, HagerstownGoogle Scholar
  30. Khan M, Tanaka S, Kligerman M, Yuhas J (1978) Responses of multicellular tumour spheroids to fractionated doses of negative pi-mesons. Radiat Res 74: 565–566Google Scholar
  31. Kopp J (1978) Radiation biological investigations with multicellular spheroids as an in vitro model. Stud Biophys 68: 159–162Google Scholar
  32. Lightdale C, Lipkin M (1975) Cell division and tumour growth. In: Becker F (ed) Cellular biology and growth. Plenum, New York, pp 201–215, (Cancer, vol 3 )Google Scholar
  33. MacPherson I (1973) Soft agar techniques. In: Kruse PF, Patterson MK (eds) Tissue culture methods and applications. Academic Press, London New York, pp 276–280Google Scholar
  34. Malaise EP, Chavaudra N, Tubiana M (1973) The relationship between growth rate, labelling index and histological type of human solid tumours. Eur J Cancer 9: 305–312PubMedCrossRefGoogle Scholar
  35. Malmqvist M (1977) Degradation of agarose by bacterial enzymes. Acta Univ Upsal 406Google Scholar
  36. McAllister RM, Reed G (1968) Colonial growth in agar of cells derived from neoplastic and non-neoplastic tissues of children. Pediatr Res 2: 356–360PubMedCrossRefGoogle Scholar
  37. McAllister RM, Reed G, Huebner RJ (1967) Colonial growth in agar of cells derived from adenovirus induced hamster tumours. J Natl Cancer Inst 39: 43–53PubMedGoogle Scholar
  38. Nederman T, Carlsson J, Malmqvist M (1981) Penetration of substances into tumour tissue. A methodological study on cellular spheroids. In Vitro 17: 290–298Google Scholar
  39. Nilsson S, Carlsson J, Larsson B, Pontén J (1980) Survival of irradiated glia and glioma cells studied with a new cloning technique. Int J Radiat Biol 37: 267–279CrossRefGoogle Scholar
  40. Rubin P, Casarett GW (1968) Clinical radiation pathology. Saunders, PhiladelphiaGoogle Scholar
  41. Shaw MT, Palmer C, Yuhas JM, Rembe A (1978) B-cell leukemia lymphoma with striking resemblance to Burkitt’s Lymphoma in a 70-year-old woman. Am J Hematol 5: 155–162PubMedCrossRefGoogle Scholar
  42. Sridhar R, Barratt DG, Grant CWM (1979) Infiltration of multicellular spheroids by lipid vesicles. Fed Proc 38: 370Google Scholar
  43. Steel GG (1977) Growth kinetics of tumours. Clarendon, OxfordGoogle Scholar
  44. Sutherland RM (1974) Selective chemotherapy of non-cycling cells in an in vitro tumour model. Cancer Res 34: 3501–3503PubMedGoogle Scholar
  45. Sutherland RM, Durand RE (1971) Radiation response of multicell spheroids in tissue culture as a model of nodular carcinomas. J Natl Cancer Inst 46: 113–120PubMedGoogle Scholar
  46. Sutherland RM, Durand RE (1976) Radiation response of multicell spheroids. An in vitro tumour model. Curr Top Radiat Res 11: 87–139Google Scholar
  47. Sutherland RM, Eddy HA, Bareham B, Reich K, Vanartwerp D (1979) Resistance to adriamycin in multicellular spheroids. Int J Radiat Oncol Biol Phys 5: 1225–1230PubMedGoogle Scholar
  48. Tannock I (1968) The relation between cell proliferation and the vascular system in a transplanted mouse mammary tumour. Br J Cancer 22: 258–273PubMedCrossRefGoogle Scholar
  49. Thomlinson RH, Gray LH (1955) The histologic structure of some human lung cancers and the possible implications for radiotherapy. Br J Cancer 9: 539–549PubMedCrossRefGoogle Scholar
  50. Waldenström JG (1978) Paraneoplasia. Biological signals in the diagnosis of cancer. Wiley, New YorkGoogle Scholar
  51. Wollard J J (1980) Nutritional management of the cancer patient. Raven, New YorkGoogle Scholar
  52. Yuhas JM (1983) Clonogenic assays for predicting the response of solid tumors to therapy. In: Fletcher G (ed) Biological bases and clinical implications of tumor radioresistance. Masson, New York, pp 383–390Google Scholar
  53. Yuhas JM, Li AP (1978) Growth fraction as the major determinant of multicellular tumor spheroid growth rates. Cancer Res 38: 1528–1532PubMedGoogle Scholar
  54. Yuhas JM, Tarleton AE (1978) Dormancy and spontaneous recurrence of human breast cancer in vitro. Cancer Res 38: 3584–3589PubMedGoogle Scholar
  55. Yuhas JM, Li AP, Martinez AO, Ladman A J (1977) A simplified method for production and growth of multicellular tumour spheroids. Cancer Res 37: 3639–3643PubMedGoogle Scholar
  56. Yuhas JM, Tarleton AE, Hartman JG (1978a) In vitro analysis of the response of multicellular tumour spheroids exposed to chemotherapeutic agents in vitro or in vivo. Cancer Res 38: 3595–3598PubMedGoogle Scholar
  57. Yuhas JM, Tarleton AE, Molzen KB (1978b) Multicellular tumor spheroid formation by breast cancer cells isolated from different sites. Cancer Res 38: 2486–2491PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1984

Authors and Affiliations

  • J. Carlsson
    • 1
  • J. M. Yuhas
    • 1
  1. 1.Department of Radiation Biology (FOA 4)National Defence Research InstituteUmeåSweden

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