The Mystery of Virus Trafficking Into, Through and Out of Vascular Tissue

  • Richard S. Nelson
  • Aart J. E. van Bel
Part of the Progress in Botany book series (BOTANY, volume 59)


A simple way to look at the process of plant virus infection is as a board or computer game. The objective for the virus is to move from the site of inoculation throughout the rest of the host plant, replicating and accumulating at specific areas along the way. The host plant serves as the game board. To “win” this game, a virus must find the most efficient way to infect the entire plant. Since viruses encode only a portion of the proteins necessary for this challenge, they must enlist (i.e. usurp) plant host factors to aid them in this journey. They also must avoid defence mechanisms employed by the host to limit their systemic spread.


Mosaic Virus Tobacco Mosaic Virus Cucumber Mosaic Virus Sieve Tube Minor Vein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Allison R, Thompson C, Ahlquist P (1990) Regeneration of a functional RNA virus genome by recombination between deletion mutants and requirement for cowpea chlorotic mottle virus 3a and coat genes for systemic infection. Proc Natl Acad Sci USA 87: 1820–1824PubMedGoogle Scholar
  2. Altus DP, Canny MJ (1982) Loading of assimilates in wheat leaves I. The specialization of vein types for separate activities. Aust J Plant Physiol 9: 571–581Google Scholar
  3. Ammerlaan A, Kempers R, van Bel AJE (1996) Symplasmic isolation of sieve element-companion cell complex in stem is universal. J Exp Bot 47: 1300Google Scholar
  4. Anderson LS, Dale JE (1983) The sources of carbon for developing leaves of barley. J Exp Bot 34: 405–414Google Scholar
  5. Arce-Johnson P, Reimann-Philipp U, Padgett HS, Rivera-Bustamente R, Beachy RN (1997) Requirement of the movement protein for long distance spread of tobacco mosaic virus in grafted plants. Mol Plant Microbe Interact (in press)Google Scholar
  6. Atabekov JG, Dorokhov YL (1984) Plant virus-specific transport function and resistance of plants to viruses. Adv Virus Res 29: 313–364PubMedGoogle Scholar
  7. Atreya CD, Raccah B, Pirone TP (1990) A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology 178: 161–165PubMedGoogle Scholar
  8. Atreya PL, Atreya CD, Pirone TP (1991) Amino acid substitutions in the coat protein result in loss of insect transmissibility of a plant virus. Proc Natl Acad Sci USA 88: 7887–7891PubMedGoogle Scholar
  9. Azzam O, Frazer J, de la Rosa D, Beaver JS, Ahlquist P, Maxwell DP (1994) Whitefly transmission and efficient ssDNA accumulation of bean golden mosaic geminivirus require functional coat protein. Virology 204: 289–296PubMedGoogle Scholar
  10. Bao Y, Carter SA, Nelson RS (1996) The 126- and 183-kilodalton proteins of tobacco mosaic virus, and not their common nucleotide sequence, control mosaic symptom formation in tobacco. J Virol 70: 6378–6383PubMedGoogle Scholar
  11. Barker H (1987a) Invasion of non-phloem tissue in Nicotiana clevelandii by potato leafroll luteovirus is enhanced in plants also infected with potato Y potyvirus. J Gen Virol 68: 1223–1227Google Scholar
  12. Barker H (1987b) Multiple components of the resistance of potatoes to potato leafroll virus. Ann Appl Biol 111: 641–648Google Scholar
  13. Barker H, Harrison BD (1982) Infection of potato mesophyll protoplasts with five plant viruses. Plant Cell Rep 1: 247–249Google Scholar
  14. Beebe DU, Evert RF (1992) Photoassimilate pathway(s) and phloem loading in the leaf of Moricandia arvensis (L.) DC. (Brassicaceae). Int J Plant Sci 153: 61–77Google Scholar
  15. Bennett CW (1940) Relation of food translocation to movement of virus of tobacco mosaic. J Agric Res 60: 361–389Google Scholar
  16. Bennett CW (1956) Biological relations of plant viruses. Annu Rev Plant Physiol 7:143–170Google Scholar
  17. Blakeslee AF (1921) A graft-infectious disease of Datura resembling a vegetative mutation. J Genet 11: 17–36Google Scholar
  18. Boccard F, Baulcombe D (1993) Mutational analysis of cis-acting sequences and gene function in RNA3 of cucumber mosaic virus. Virology 193: 563–578PubMedGoogle Scholar
  19. Bosabalidis AM, Evert RF, Russin WA (1994) Ontogeny of the vascular bundles and contiguous tissues in the maize leaf blade. Am J Bot 81: 745–752Google Scholar
  20. Botha CEJ (1992) Plasmodesmatal distribution, structure and frequency in relation to assimilation in C3 and C4 grasses in southern Africa. Planta 187: 348–358Google Scholar
  21. Botha CEJ, Evert RF (1988) Plasmodesmatal distribution and frequency in vascular bundles and contiguous tissues of the leaf of Themeda triandra. Planta 173: 433–441Google Scholar
  22. Botha CEJ, van Bel AJE (1992) Quantification of symplastic continuity as visualised by plasmodesmograms: diagnostic value for phloem-loading pathways. Planta 187: 359–366Google Scholar
  23. Boulton MI, Steinkellner H, Donson J, Markham PG, King DI, Davies JW (1989) Mutational analysis of the virion-sense genes of maize streak virus. J Gen Virol 70: 2309–2323PubMedGoogle Scholar
  24. Bourquin S, Bonnemain J-L, Delrot S (1990) Inhibition of loading of,4C assimilates by p-chloromercuribenzenesulfonic acid. Localization of the apoplastic pathway in Vicia faba. Plant Physiol 92: 97–102PubMedGoogle Scholar
  25. Bransom KL, Weiland JJ, Tsai CH, Dreher TW (1995) Coding density of the turnip yellow mosaic virus genome: roles of the overlapping coat protein and p206-readthrough coding regions. Virology 206: 403–412PubMedGoogle Scholar
  26. Briddon RW, Watts J, Markham PG, Stanley J (1989) The coat protein of beet curly top virus is essential for infectivity. Virology 172: 628–633PubMedGoogle Scholar
  27. Brough CL, Hayes RJ, Morgan AJ, Coutts RHA, Buck KW (1988) Effects of mutagenesis in vitro on the ability of cloned tomato golden mosaic virus DNA to infect Nicotiana benthamiana plants. J Gen Virol 69: 503–514Google Scholar
  28. Brugidou C, Holt C, Yassi MNA, Zhang S, Beachy R, Fauquet C (1995) Synthesis of an infectious full-length cDNA clone of rice yellow mottle virus and mutagenesis of the coat protein. Virology 206: 108–115PubMedGoogle Scholar
  29. Buck KW (1996) Comparison of the replication of positive-stranded RNA viruses of plants and animals. Adv Virus Res 47: 159–251PubMedGoogle Scholar
  30. Burgyán J, Tavazza M, Dalmay T, Lucioli A, Baldz S (1993) Consequences of gene transfer between distantly related tombusviruses. Gene 129: 191–196PubMedGoogle Scholar
  31. Caldwell J (1930) The physiology of virus diseases in plants I. The movement of mosaic in the tomato plant. Ann Appl Biol 17: 429–443Google Scholar
  32. Caldwell J (1934) The physiology of virus diseases in plants V. The movement of the virus agent in tobacco and tomato. Ann Appl Biol 21: 191–205Google Scholar
  33. Callaway A, Liu W, Andrianov V, Stenzler L, Zhao J, Wettlaufer S, Jayakumar P, Howell SH (1996) Characterization of cauliflower mosaic virus (CaMV) resistance in virus- resistant ecotypes of Arabidopsis. Mol Plant Microbe Interact 9: 810–818PubMedGoogle Scholar
  34. Canny MJ (1990) What becomes of the transpiration stream? New Phytol 114: 341–368Google Scholar
  35. Carr JP, Zaitlin M (1991) Resistance in transgenic tobacco plants expressing a nonstructural gene sequence of tobacco mosaic virus is a consequence of markedly reduced virus replication. Mol Plant Microbe Interact 4: 579–585Google Scholar
  36. Carr RJ, Kim KS (1983) Evidence that bean golden mosaic virus invades non-phloem tissue in double infections with tobacco mosaic virus. J Gen Virol 64: 2489–2492Google Scholar
  37. Carrington JC, Kasschau KD, Mahajan SK, Schaad MC (1996) Cell-to-cell and longdistance transport of viruses in plants. Plant Cell 8: 1669–1681PubMedGoogle Scholar
  38. Carrington WA, Lynch RM, Moore EDW, Isenberg G, Fogarty KE, Fay FS (1995) Super-resolution three-dimensional images of fluorescence in cells with minimal light exposure. Science 268: 1483–1487PubMedGoogle Scholar
  39. Cartwright SC, Lush WM, Canny M (1977) A comparison of translocation of labelled assimilate by normal and lignified sieve elements in wheat leaves. Planta 134: 207–208Google Scholar
  40. Casavan W, Kramer J, Hitrys D (1996) High-resolution 3-D fluorescence microscopy: a comparison of confocal laser scanning microscopy and a wide-field deconvolution technique. Am Biotechnol Lab 14: 12–16Google Scholar
  41. Chambers TC, Francki RIB (1966) Localization and recovery of lettuce necrotic yellows virus from xylem tissues of Nicotiana glutinosa. Virology 29: 673–676PubMedGoogle Scholar
  42. Chapman S, Hills G, Watts J, Baulcombe D (1992a) Mutational analysis of the coat protein gene of potato virus X: effects on virion morphology and viral pathogenicity. Virology 191: 223–230PubMedGoogle Scholar
  43. Chapman S, Kavanagh T, Baulcombe D (1992b) Potato virus X as a vector for gene expression in plants. Plant J 2: 549–557PubMedGoogle Scholar
  44. Chonan N, Kawahara H, Matsuda T (1984) Ultrastructure of vascular bundles and fundamental parenchyma in relation to movement of photosynthate in leaf sheath of rice. Jpn J Crop Sci 53: 435–444Google Scholar
  45. Chonan N, Kawahara H, Matsuda T (1985) Ultrastructure of transverse veins in relation to phloem loading in the rice leaf. Jpn J Crop Sci 54: 160–169Google Scholar
  46. Citovsky V, Zambryski P (1993) Transport of nucleic acids through membrane channels: snaking through small holes. Annu Rev Microbiol 47: 167–197PubMedGoogle Scholar
  47. Cleland RF, Fujiwara T, Lucas WJ (1994) Plasmodesmal-mediated cell-to-cell transport in wheat roots is modulated by anaerobic stress. Protoplasma 178: 81–85PubMedGoogle Scholar
  48. Colbert JT, Evert RF (1982) Leaf vasculature in sugarcane (Saccharum officinarum L.) Planta 156: 136–151Google Scholar
  49. Covey SN, Hull R (1992) Genetic engineering with double-stranded DNA viruses. In: Wilson TMA, Davies JW (eds) Genetic engineering with plant viruses. CRC Press, Boca Raton, pp 217–249Google Scholar
  50. Crameri A, Whitehorn EA, Tate E, Stemmer WPC (1996) Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat Biotechnol 14: 315–319PubMedGoogle Scholar
  51. Cronin S, Verchot J, Haldemann-Cahill R, Schaad MC, Carrington JC (1995) Long distance movement factor: a transport function of the potyvirus helper component proteinase. Plant Cell 7: 549–559PubMedGoogle Scholar
  52. D’Arcy CJ, de Zoeten GA (1979) Best western yellows virus in phloem tissue of Thlaspi arvense. Phytopathology 69: 1194–1198Google Scholar
  53. Dalmay T, Rubino L, Burgydn J, Russo M (1992) Replication and movement of a coat protein mutant of cymbidium ringspot tombusvirus. Mol Plant Microbe Interact 5: 379 - 383PubMedGoogle Scholar
  54. Dannenhoffer JM, Evert RF (1994) Development of the vascular system in the leaf of barley. Int J Plant Sci 155: 143–157Google Scholar
  55. Dannenhoffer JM, Ebert W, Evert RF (1990) Leaf vasculature in barley, Hordeum vulgare (Poaceae). Am J Bot 77: 636–652Google Scholar
  56. Dawson WO, Hilf ME (1992) Host-range determinants of plant viruses. Annu Rev Plant Physiol Plant Mol Biol 43: 527–557Google Scholar
  57. Dawson WO, Schlegel DE (1973) Differential temperature treatment of plants greatly enhances multiplication rates. Virology 53: 476–478PubMedGoogle Scholar
  58. Dawson WO, Bubrick P, Grantham GI (1988) Modification of the tobacco mosaic virus coat protein gene affecting replication, movement, and symptomology. Phytopathology 78: 783–789Google Scholar
  59. De Boer AH, Wegner LH (1997) Regulatory mechanisms of ion channels in xylem parenchyma cells. J Exp Bot 48 (in press)Google Scholar
  60. De Jong W, Ahlquist P (1995) Host-specific alterations in viral RNA accumulation and infection spread in a brome mosaic virus isolate with an expanded host range. J Virol 69: 1485–1492PubMedGoogle Scholar
  61. Dengler NG, Dengler RE, Hattersley PW (1985) Differing ontogenic origins of PCR (‘Kranz’) sheaths in leaf blades of C4 grasses (Poaceae). Am J Bot 72: 284–302Google Scholar
  62. Deom CM, Lapidot M, Beachy RN (1992) Plant virus movement proteins. Cell 69: 221–224PubMedGoogle Scholar
  63. Deom CM, He XZ, Beachy RN, Weissinger AK (1994) Influence of heterologous to- bamovirus movement protein and chimeric-movement protein genes on cell-to-cell and long-distance movement. Virology 205: 198–209PubMedGoogle Scholar
  64. Derrick PM, Barker H (1992) The restricted distribution of potato leafroll luteovirus antigen in potato plants with transgenic resistance resembles that in clones with one type of host gene-mediated resistance. Ann Appl Biol 120: 451–457Google Scholar
  65. Derrick PM, Barker H (1997) Short and long distance spread of potato leafroll luteovirus: effects of host genes and transgenes conferring resistance to virus accumulation in potato. J Gen Virol 78: 243–251PubMedGoogle Scholar
  66. Derrick PM, Barker H, Oparka KJ (1992) Increase in plasmodesmatal permeability during cell-to-cell spread of tobacco rattle virus from individually inoculated cells. Plant Cell 4: 1405–1412PubMedGoogle Scholar
  67. Derrick PM, Carter SA, Nelson RS (1997) Mutation of the tobacco mosaic tobamovirus 126/183 kDa proteins: effects on phloem-dependent virus accumulation and synthesis of viral proteins. Mol Plant Microbe Interacts 10: 589–596Google Scholar
  68. De Zoeten GA (1995) Plant virus infection: another point of view. Adv Bot Res 21: 105–124Google Scholar
  69. De Zoeten GA, Gaard G (1983) Mechanisms underlying systemic invasion of pea plants by pea enation mosaic virus. Intervirology 19: 85–94PubMedGoogle Scholar
  70. Ding B (1997) Cell-to-cell transport of macromolecules through plasmodesmata: a novel signally pathway in plants. Trends Cell Biol 7: 5–9PubMedGoogle Scholar
  71. Ding B, Pathasarathy MV, Niklas K, Turgeon R (1988) A morphometric analysis of the phloem-unloading pathway in developing tobacco leaves. Planta 176: 307–318Google Scholar
  72. Ding B, Haudenshield JS, Hull RJ, WolfS, Beachy RN, Lucas WJ (1992) Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants. Plant Cell 4: 915–928PubMedGoogle Scholar
  73. Ding SW, Li WX, Symons RH (1995) A novel naturally occurring hybrid gene encoded by a plant RNA virus facilitates long distance virus movement. EMBO J 14: 5762–5772PubMedGoogle Scholar
  74. Ding XS, Shintaku MH, Arnold SA, Nelson RS (1995) Accumulation of mild and severe strains of tobacco mosaic virus in minor veins of tobacco. Mol Plant Microbe Interact 8: 32–40Google Scholar
  75. Ding XS, Shintaku MH, Carter SA, Nelson RS (1996) Invasion of minor veins of tobacco leaves inoculated with tobacco mosaic virus mutants defective in phloem-dependent movement. Proc Natl Acad Sci USA 93: 11155–11160PubMedGoogle Scholar
  76. Ding XS, Carter SA, Nelson RS (1997) Tobamovirus and potyvirus accumulation in minor veins of inoculated leaves from representatives of the Solanaceae and Fabaceae. Plant Physiol (in press)Google Scholar
  77. Dolja VV, Haldeman R, Robertson NL, Dougherty WG, Carrington JC (1994) Distinct functions of capsid protein in assembly and movement of tobacco etch potyvirus in plants. EMBO J 13: 1482–1491PubMedGoogle Scholar
  78. Dolja VV, Haldeman-Cahill R, Montgomery AE, Vandenbosch KA, Carrington JC (1995) Capsid protein determinants involved in cell-to-cell and long distance movement of tobacco etch potyvirus. Virology 206: 1007–1016PubMedGoogle Scholar
  79. Dorokhov YL, Alexandrova NM, Miroshnichenko NA, Atabekov JG (1984) Stimulation by aurintricarboxylic acid of tobacco mosaic virus-specific RNA synthesis and production of informosome-like infection-specific ribonucleoprotein. Virology 135: 395–405PubMedGoogle Scholar
  80. Dubois F, Sangwan RS, Sangwan-Norreel BS (1994) Spread of beet necrotic yellow vein virus in infected seedlings and plants of sugar beet (Beta vulgaris). Protoplasma 179: 72–82Google Scholar
  81. Dufour O, Palloix A, Selassie KG, Pochard E, Marchoux G (1989) The distribution of cucumber mosaic virus in resistant and susceptibe plants of pepper. Can J Bot 67: 655–660Google Scholar
  82. Eastman PAK, Dengler NG, Peterson CA (1988a) Suberized bundle sheaths in grasses (Poaceae) of different photosynthetic types. I. Anatomy, ultrastructure and histochemistry. Protoplasma 142: 92–111Google Scholar
  83. Eastman PAK, Peterson CA, Dengler NG (1988b) Suberized bundle sheaths in grasses (Poaceae) of different photosynthetic types. II. Apoplastic permeability. Protoplasma 142: 112–126Google Scholar
  84. Edwards MC, Gonsalves D, Prowidenti R (1983) Genetic analysis of cucumber mosaic virus in relation to host resistance: location of determinants for pathogenicity to certain legumes and Lactuca saligno. Phytopathology 73: 269–273Google Scholar
  85. Eleftheriou EP, Tsekos I (1979) Development of mestome sheath cells in leaves of Aegilops comosa var. thessalica. Protoplasma 100: 139–153Google Scholar
  86. Epel BL, van Lent JWM, Cohen L, Kotlizky G, Katz A, Yahalom A (1996) A 41 kDa protein isolated from maize mesocotyl cell walls immunolocalizes to plasmodesmata. Protoplasma 191: 70–78Google Scholar
  87. Esau K (1967) Minor veins in Beta leaves: structure related to function. Proc Am Philos Soc 111: 219–233Google Scholar
  88. Esau K (1968) Viruses in plant hosts. Form, distribution, and pathologic effects. University of Wisconsin Press, Madison, WI pp 1–225Google Scholar
  89. Esau K (1977) Anatomy of seed plants. John Wiley & Sons, New York, pp 321–332Google Scholar
  90. Esau K, Cronshaw J (1967) Relation of tobacco mosaic virus to the host cells. J Cell Biol 33: 665–678PubMedGoogle Scholar
  91. Esau K, Hoefert LL (1971) Cytology of beet yellows virus infection in Tetragonia III. Conformations of virus in infected cells. Protoplasma 73: 51–65PubMedGoogle Scholar
  92. Esau K, Cronshaw J, Hoefert LL (1967) Relation of beet yellows virus to the phloem and the movement in the sieve tube. J Cell Biol 32: 71–87PubMedGoogle Scholar
  93. Eschrich W, Evert RF, Heyser W (1971) Proteins of the sieve-tube exudate of Cucurbita maxima. Planta 100: 208–221Google Scholar
  94. Etessami P, Watts J, Stanley J (1989) Size reversion of African cassava mosaic virus coat protein gene deletion mutants during infection of Nicotiana benthamiana. J Gen Virol 70: 277–289PubMedGoogle Scholar
  95. Evert RF, Mierzwa R (1986) Pathway(s) of assimilate movement from mesophyll cells to sieve tubes in the Beta vulgaris leaf. In: Cronshaw J, Lucas WJ, Giaquinta RT (eds) Phloem transport. Liss, New York, pp 419–432\Google Scholar
  96. Evert RF, Russin WA (1993) Structurally, phloem unloading in the maize leaf cannot be symplastic. Am J Bot 80: 1310–1317Google Scholar
  97. Evert RF, Eschrich W, Heyser W (1977) Distribution and structure of the plasmodesmata in mesophyll and bundle-sheath cells of Zea mays L. Planta 136: 77–89Google Scholar
  98. Evert RF, Eschrich W, Heyser W (1978) Leaf structure in relation to solute transport and phloem loading in Zea mays L. Planta 138: 279–294Google Scholar
  99. Evert RF, Botha CEJ, Mierzwa RJ (1985) Free-space marker studies on the leaf of Zea mays L. Protoplasma 126: 62–73Google Scholar
  100. Fenczik CA, Padgett HS, Holt CA, Casper SJ, Beachy RN (1995) Mutational analysis of the movement protein of odontoglossum ringspot virus to identify a host-range determinant. Mol Plant Microbe Interact 8: 666–673PubMedGoogle Scholar
  101. Fisher DB, Wu Y, Ku MSB (1992) Turnover of soluble proteins in the wheat sieve tube. Plant Physiol 100: 1433–1441PubMedGoogle Scholar
  102. Fisher DG (1986) Ultrastructure, plasmodesmatal frequency and solute concentration in green areas of variegated Coleus blumei Benth. leaves. Planta 169: 141–152Google Scholar
  103. Fisher DG (1990) Distribution of plasmodesmata in leaves. A comparison of Cananga odarata with other species using different measures of plasmodesmatal frequency. In: Robards AW, Lucas WJ, Pitts JD, Jongsma HJ, Spray DC (eds) Parallels in cell-to-cell junctions in plants and animals. Springer, Berlin Heidelberg New York, pp 199–221Google Scholar
  104. Fisher DG, Evert RF (1982) Studies on the leaf of Amaranthus retroflexus (Amaranthaceae): ultrastructure, plasmodesmatal frequency, and solute concentration in relation to phloem loading. Planta 155: 377–387Google Scholar
  105. Flasinski S, Dzianott A, Pratt S, Bujarski JJ (1995) Mutational analysis of the coat protein gene of brome mosaic virus: effects on replication and movement in barley and in Chenopodium hybridum. Mol Plant Microbe Interact 8: 23–31PubMedGoogle Scholar
  106. Flora LL, Madore MA (1996) Significance of minor-vein anatomy to carbohydrate transport. Planta 198: 171–178Google Scholar
  107. Forster RLS, Beck DL, Guilford PJ, Voot DM, van Dolleweerd CJ, Andersen MT (1992) The coat protein of white clover mosaic potexvirus has a role in facilitating cell-to-cell transport in plants. Virology 191: 480–484PubMedGoogle Scholar
  108. Fox JM, Johnson JE, Young MJ (1994) RNA/protein interactions in icosahedral virus assembly. Semin Virol 5: 51–60Google Scholar
  109. Franceschi VR, Giaquinta RT (1983a) The paraveinal mesophyll of soybean leaves in relation to assimilate transfer and compartmentation. II. Structural, metabolic and compartmental changes during reproductive growth. Planta 157: 422–431Google Scholar
  110. Franceschi VR, Giaquinta RT (1983b) Specialized cellular arrangements in legume leaves in relation to assimilate transport and compartmentation: comparison of the paraveinal mesophyll. Planta 159: 415–422Google Scholar
  111. Fritz E, Evert RF, Heyser W (1983) Microautoradiographic studies of phloem loading and transport in the leaf of Zea mays L. Planta 159: 193–206Google Scholar
  112. Fritz E, Evert RF, Nasse H (1989) Loading and transport of assimilates in different maize leaf bundles. Digital image analysis of l4C-microautoradiographs. Planta 178: 1–9Google Scholar
  113. Fry PR, Matthews REF (1963) Timing of some early events following inoculation with tobacco mosaic virus. Virology 19: 461–469PubMedGoogle Scholar
  114. Fuentes AL, Hamilton RI (1993) Failure of long-distance movement of southern bean mosaic virus in a resistant host correlated with lack of normal virion formation. J Gen Virol 74: 1903–1910PubMedGoogle Scholar
  115. Fujiwara T, Giesman-Cookmeyer D, Ding B, Lommel SA, Lucas WJ (1993) Cell-to-cell trafficking of macromolecules through plasmodesmata potentiated by the red clover necrotic mosaic virus movement protein. Plant Cell 5: 1783–1794PubMedGoogle Scholar
  116. Gal-On A, Kaplan I, Roossnick MJ, Palukaitis P (1994) The kinetics of infection of zucchini squash by cucumber mosaic virus indicate a function for RNA 1 in virus movement. Virology 205: 280–289PubMedGoogle Scholar
  117. Gamalei YV (1989) Structure and function of leaf minor veins in trees and herbs. A taxonomic review. Trees 3: 96–110Google Scholar
  118. Gamalei YV (1990) Leaf phloem. Nauka, Leningrad (in Russian)Google Scholar
  119. Gamalei YV, Pakhomova MV, Sjutkina AV (1992) Ecological aspects of assimilate trans-port. I. Temperature. Fiziol Rast 39: 1068–1078Google Scholar
  120. Gamalei YV, van Bel AJE, Pakhomova MV, Sjutkina AV (1994) Effects of temperature on the conformation of the endoplasmic reticulum and on starch accumulation in leaves with the symplasmic minor-vein configuration. Planta 194: 443–453Google Scholar
  121. Gardiner WE, Sunter G, Brand L, Elmer JS, Rogers SG, Bisaro DM (1988) Genetic analysis of tomato golden mosaic virus: the coat protein is not required for systemic spread or symptom development. EMBO J 7: 899–904PubMedGoogle Scholar
  122. Gera A, Deom CM, Donson J, Shaw JJ, Lewandowski DJ, Dawson WO (1995) Tobacco mosaic tobamovirus does not require concomitant synthesis of movement protein during vascular transport. Mol Plant Microbe Interact 8: 784–787Google Scholar
  123. Gergerich RC, Scott HA (1988) Evidence that virus transmission and virus infection of non-wounded cells are associated with transmissibility by leaf-feeding beetles. J Gen Virol 69: 2935–2938Google Scholar
  124. Gilbertson RL, Lucas WJ (1996) How do viruses traffic on the Vascular highway? Trends Plant Sci 1: 260–267Google Scholar
  125. Goodrick BJ, Kuhn CW, Hussey RS (1991) Restricted systemic movement of cowpea chlorotic mottle virus in soybean with nonnecrotic resistance. Phytopathology 81: 1426–1431Google Scholar
  126. Goulden MG, Baulcombe DC (1993) Functionally homologous host components recognize potato virus X in Gomphrena globosa and potato. Plant Cell 5: 921–930PubMedGoogle Scholar
  127. Grusak MA, Beebe DU, Turgeon R (1996) Phloem loading: In: Zamski E, Schaffer AA (eds) Photoassimilate distribution in plants and crops. Dekker, New York, pp 209–227Google Scholar
  128. Hacker DL, Petty ITD, Wei N, Morris TJ (1992) Turnip crinkle virus genes required for RNA replication and virus movement. Virology 186: 1–8PubMedGoogle Scholar
  129. Hamilton WDO, Baulcombe DC (1989) Infectious RNA produced by in vitro transcription of a full-length tobacco rattle virus RNA-1 cDNA. J Gen Virol 70: 963–968Google Scholar
  130. Hassan S, Thomas PE (1988) Extreme resistance in tomato yellow top virus and potato leaf roll virus in Lycopersicon peruvianum and some of its tomato hybrids. Phytopathology 78: 1164–1167Google Scholar
  131. Hatta T, Matthews REF (1974) The sequence of early cytological changes in Chinese cabbage leaf cells following systemic infection with turnip yellow moasic virus. Virology 59: 383–396PubMedGoogle Scholar
  132. Hayes PM, Offler CE, Patrick JW (1985) Cellular structures, plasma membrane surface areas and plasmodesmatal frequencies of the stem of Phaseolus vulgaris in relation to radial photosynthate transfer. Ann Bot 56: 125–138Google Scholar
  133. Hayes PM, Patrick JW, Offler CE (1987) The cellular pathway of radial transfer in stems of Phaseolus vulgaris L.: effects on cellular plasmolysis and p-chloromercuribenzene- sulphonic acid. Ann Bot 59: 635–642Google Scholar
  134. Hayes RJ, Buck KW (1990) Complete replication of a eukaryotic virus RNA in vitro by a purified RNA-dependent RNA polymerase. Cell 63: 363–368PubMedGoogle Scholar
  135. Heaton LA, Morris TJ (1992) Structural implications for spherical plant virus disassembly in vivo. Semin Virol 3: 433–439Google Scholar
  136. Heaton LA, Lee TC, Wei N, Morris TJ (1991) Point mutations in the turnip crinkle virus capsid protein affect the symptoms expressed by Nicotiana benthamiana. Virology 183: 143–150PubMedGoogle Scholar
  137. Hickey LJ (1979) A revised classification of the architecture of dicotyledonous leaves. In: Metcalfe CR, Chalk L (eds) Anatomy of the dicotyledons. Oxford University Press, New York, pp 25–39Google Scholar
  138. Hilf ME, Dawson WO (1993) The tobamovirus capsid protein functions as a host-specific determinant of long-distance movement. Virology 193: 106–114PubMedGoogle Scholar
  139. Hoefert LL, Esau K, Duffiis JE (1970) Electron microscopy of Beta leaves infected with beet yellow stunt virus. Virology 42: 814–824PubMedGoogle Scholar
  140. Hoefert LL, Pinto RL, Fail GL (1988) Ultrastructural effects of lettuce infectious yellows virus in Lactuca sativa L. J Ultrastruct Mol Struct Res 98: 243–253Google Scholar
  141. Holmes FO (1930) Local and systemic increase of tobacco mosaic virus. Am J Bot 17: 789–805Google Scholar
  142. Holmes FO (1932) Movement of mosaic virus from primary lesions in Nicotiana tabacum L. Contrib Boyce Thompson Inst 4: 297–322Google Scholar
  143. Holmes FO (1934) A masked strain of tobacco mosaic virus. Phytopathology 24: 845–873Google Scholar
  144. Holmes FO (1955) Additive resistances to specific viral diseases in plants. Ann Appl Biol 42: 129–139Google Scholar
  145. Horner HT, Lersten NR, Wirth CL (1994) Quantitative survey of sieve tube distribution in foliar terminal veins of ten dicot species. Am J Bot 81: 1267–1274Google Scholar
  146. Ingham DJ, Pascal E, Lazarowitz SG (1995) Both bipartite geminivirus movement proteins define viral host range, but only BL1 determines viral pathogenicity. Virology 207: 191–204PubMedGoogle Scholar
  147. Ishiwatari Y, Honda C, Kawashima I, Nakamura S, Hirano H, Mori S, Fujiwara T, Hayashi H, Chino M (1995) Thioredoxin h is one of the major proteins in rice phloem sap. Planta 195: 456–463PubMedGoogle Scholar
  148. Jacobsen KR, Fisher DG, Maretzki A, Moore PH (1992) Developmental changes in the anatomy of the sugarcane stem in relation to phloem unloading and sucrose storage. Bot Acta 105: 70–80Google Scholar
  149. Jeffrey JL, Pooma W, Petty ITD (1996) Genetic requirements for local and systemic movement of tomato golden mosaic virus in infected plants. Virology 223: 208–218PubMedGoogle Scholar
  150. Kaneko M, Chonan N, Matsuda T, Kawahara H (1980) Ultrastructure of small vascular bundles and transfer pathways for photosynthate in the leaves of rice plant. Jpn J Crop Sci 49: 42–50Google Scholar
  151. Kempers R, van Bel A JE (1997) Symplasmic connections between sieve element and companion cell in the stem phloem of Vicia faba L. have a molecular exclusion limit of a least 10 kDa. Planta 201: 195–201Google Scholar
  152. Kempers R, Prior DAM, van Bel AJE, Oparka KJ (1993) Plasmodesmata between sieve element and companion cell of extrafascicular stem phloem of Cucurbita maxima permit passage of 3 kDa fluorescent probes. Plant J 4: 567–575Google Scholar
  153. Köhm BA, Goulden MG, Gilbert JE, Kavanagh TA, Baulcombe DC (1993) A potato virus X resistance gene mediates an induced, nonspecific resistance in protoplasts. Plant Cell 5: 913–920PubMedGoogle Scholar
  154. Komor E (1977) Sucrose uptake by cotyledons of Ricinus communis L: characteristics, mechanism, and regulation. Planta 137: 119–131Google Scholar
  155. Kuhn CW, Wyatt SD, Brantley BB (1981) Genetic control of symptoms, movement, and virus accumulation in cowpea plants infected with cowpea chlorotic mottle virus. Phytopathology 71: 1310–1315Google Scholar
  156. Kuo J, O’Brien TP (1974) Lignified sieve elements in the wheat leaf. Planta 117: 349–353Google Scholar
  157. Kuo J, O’Brien TP, Canny MJ (1974) Pit-field distribution, plasmodesmatal frequency, and assmilate flux in the mestome sheath cells of wheat leaves. Planta 121: 97–118Google Scholar
  158. Laakso MM, Heaton LA (1993) Asp -» Asn substitutions in the putative calcium-binding site of the turnip crinkle virus coat protein affect virus movement in plants. Virology 197: 774–777PubMedGoogle Scholar
  159. Lakshman DK, Gonsalves D (1985) Genetic analyses of two large-lesion isolates of cucumber mosaic virus. Phytopathology 75: 758–762Google Scholar
  160. Law MD, Moyer JW, Payne GA (1989) Effect of host resistance on pathogenesis of maize dwarf mosaic virus. Phytopathology 79: 757–761Google Scholar
  161. Lazarowitz SG (1992) Geminiviruses: Genome structure and gene function. Crit Rev Plant Sci 11: 327–349Google Scholar
  162. Lazarowitz SG, Pinder AJ, Damsteegt VD, Rogers SG (1989) Maize streak virus genes essential for systemic spread and symptom development. EMBO J 8: 1023–1032PubMedGoogle Scholar
  163. Lei JD, Agrios GN (1986) Mechanisms of resistance in corn to maize dwarf mosaic virus. Phytopathology 76: 1034–1040Google Scholar
  164. Leisner SM, Turgeon R (1993) Movement of virus and photoassimilate in the phloem: a comparative analysis. Bioessays 15: 741–748PubMedGoogle Scholar
  165. Leisner SM, Turgeon R, Howell SH (1992) Long distance movement of cauliflower mosaic virus in infected turnip plants. Mol Plant Microbe Interact 5: 41–47Google Scholar
  166. Leisner SM, Turgeon R, Howell SH (1993) Effects of host plant development and genetic determinants on the long-distance movement of cauliflower mosaic virus in Arabidopsis. Plant Cell 5: 191–202PubMedGoogle Scholar
  167. Lersten NR (1990) Sieve tubes in foliar vein endings: review and quantitative survey of Rudbeckia laciniata (Asteraceae). Am J Bot 77: 1132–1141Google Scholar
  168. Lewandowski DJ, Dawson WO (1993) A single amino acid change in tobacco mosaic virus replicase prevents symptom production. Mol Plant Microbe Interact 6: 157–160Google Scholar
  169. Lucas WJ (1995) Plasmodesmata: intercellular channels for macromolecular transport in plants. Curr Opin Cell Biol 7: 673–680PubMedGoogle Scholar
  170. Lucas WJ, Gilbertson RL (1994) Plasmodesmata in relation to viral movement within leaf tissues. Annu Rev Phytopathol 32: 387–411Google Scholar
  171. Lucas WJ, Wolf S (1993) Plasmodesmata: the intercellular organelles of green plants. Trends Cell Biol 3: 308–315PubMedGoogle Scholar
  172. Lucy AP, Boulton MI, Davies JW, Maule AJ (1996) Tissue specificity of Zea mays infection by maize streak virus. Mol Plant Microbe Interact 9: 22–31Google Scholar
  173. Lush WM (1976) Leaf structure and translocation of dry matter in a C3 and a C4 grass. Planta 130: 235–244Google Scholar
  174. Maule AJ (1991) Virus movement in infected plants. Crit Rev Plant Sci 9: 457–473Google Scholar
  175. Mauseth JD (1988) Plant Anatomy. Benjamin/Cummings, Menlo Park, CA, pp 238–244Google Scholar
  176. McCauley MM, Evert RF (1989) Minor veins of the potato (Solanum tuberosum L.) leaf: ultrastructure and plasmodesmatal frequency. Bot Gaz 150: 351–368Google Scholar
  177. Melcher U (1989) Symptoms of cauliflower mosaic virus infection in Arabidopsis thaliana and turnip. Bot Gaz 150: 139–147Google Scholar
  178. Minchin PEH, Thorpe MR (1987) Measurement of unloading and reloading of photo-assimilate within the stem of bean. J Exp Bot 38: 211–220Google Scholar
  179. Mise K, Ahlquist P (1995) Host-specificity restriction by bromovirus cell-to-cell movement protein occurs after initial cell-to-cell spread of infection in nonhost plants. Virology 206: 276–286PubMedGoogle Scholar
  180. Miyake H, Maeda E (1976) The fine structure of plastids in various tissues in the leaf blade of rice. Ann Bot 40: 1131–1138Google Scholar
  181. Mundry KW, Watkins PAC, Ashfield T, Piaskitt KA, Eisele-Walter S, Wilson TMA (1991) Complete uncoating of the 5′ leader sequence of tobacco mosaic virus RNA occurs rapidly and is required to initiate cotranslational virus disassembly in vitro. J Gen Virol 72: 769–777PubMedGoogle Scholar
  182. Murphy JF, Kyle MM (1995) Alleviation of restricted systemic spread of pepper mottle potyvirus in Capsicum annuum cv. Avelar by coinfection with a cucumovirus. Phytopathology 85: 561–566Google Scholar
  183. Nelson RS, Li G, Hodgson RAJ, Beachy RN, Shintaku MH (1993) Impeded phloem-dependent accumulation of the masked strain of tobacco mosaic virus. Mol Plant Microbe Interact 6: 45–54Google Scholar
  184. Nilsson-Tillgren T, Kolehmainen-Sevrus L, von Wettstein D (1969) Studies on the bio-synthesis of TMV I. A system approaching a synchronized virus synthesis in a tobacco leaf. Mol Gen Genet 104: 124–141PubMedGoogle Scholar
  185. Nishiguchi M, Kikuchi S, Kiho Y, Ohno T, Meshi T, Okada Y (1985) Molecular basis of plant viral virulence: the complete nucleotide sequence of an attenuated strain of tobacco mosaic virus. Nucleic Acids Res 13: 5585–5590PubMedGoogle Scholar
  186. Oparka KJ, Viola R, Wright KM, Prior DAM (1992) Sugar transport and metabolism in the potato tuber. In: Pollock CJ, Farrar IF, Gordon AJ (eds) Carbon partitioning within and between organisms. BIOS, Oxford, pp 91–114Google Scholar
  187. Oparka KJ, Duckett CM, Prior DAM, Fisher DB (1994) Real-time imaging of phloem unloading in the root tip of Arabidopsis. Plant J 6: 759–766Google Scholar
  188. Oparka KJ, Prior DAM, Wright KM (1995a) Symplastic communication between primary and developing lateral roots of Arabidopsis thaliana. J Exp Bot 46: 187–197Google Scholar
  189. Oparka KJ, Roberts AG, Prior DAM, Chapman S, Baulcombe D, Santa Cruz S (1995b) Imaging the green fluorescent protein in plants - viruses carry the torch. Protoplasma 189: 133–141Google Scholar
  190. Oparka KJ, Boevink P, Santa Cruz S (1996) Studying the movement of plant viruses using green fluorescent protein. Trends Plant Sci 1: 412–418Google Scholar
  191. Overall RL, Blackman LM (1996) A model of the macromolecular structure of plasmodemata. Trends Plant Sci 1: 307–311Google Scholar
  192. Oxelfelt P (1970) Development of systemic tobacco mosaic virus infection. I. Initiation of infection and time course of virus multiplication. Phytopathol Z 69: 202–211Google Scholar
  193. Oxelfelt P (1975) Development of systemic tobacco mosaic virus infection. IV. Synthesis of viral RNA and intact virus and systemic movement of two strains as influenced by temperature. Phytopathol Z 83: 66–76Google Scholar
  194. Padidam M, Beachy RN, Fauquet CM (1995) Tomato leaf curl geminivirus from India has a bipartite genome and coat protein is not essential for infectivity. J Gen Virol 76: 25–35PubMedGoogle Scholar
  195. Palukaitis P (1987) Potato spindle tuber viroid: investigation of the long-distance, intra-plant transport route. Virology 158: 239–241PubMedGoogle Scholar
  196. Pang S-Z, DeBoer DL, Wan Y, Ye G, Layton JG, Neher MK, Armstrong CL, Fry JE, Hinchee MAW, Fromm ME (1996) An improved green fluorescent protein gene as a vital marker in plants. Plant Physiol 112: 893–900PubMedGoogle Scholar
  197. Patrick JW, Offler CE (1996) Post-sieve element transport of photoassimilates in sink regions. J Exp Bot 47: 1165–1177PubMedGoogle Scholar
  198. Petty ITD, Jackson AO (1990) Mutational analysis of barley stripe mosaic virus RNA ß. Virology 179: 712–718PubMedGoogle Scholar
  199. Poethig RS, Sussex IM (1985a) The cellular parameters of leaf development in tobacco: a clonal analysis. Planta 165: 170–184Google Scholar
  200. Poethig RS, Sussex IM (1985b) The developmental morphology and growth dynamics of the tobacco leaf. Planta 165: 158–169Google Scholar
  201. Pooma W, Gillette WK, Jeffrey JL, Petty ITD (1996) Host and viral factors determine the dispensability of coat protein for bipartite geminivirus systemic movement. Virology 218: 264–268PubMedGoogle Scholar
  202. Powell CA, de Zoeten GA (1977) Replication of pea enation mosaic virus RNA in isolated pea nuclei. Proc Natl Acad Sci USA 74: 2919–2922PubMedGoogle Scholar
  203. Powell CA, de Zoeten GA, Gaard G (1977) The localization of pea enation mosaic virus-induced RNA-dependent RNA polymerase in infected peas. Virology 78: 135–143PubMedGoogle Scholar
  204. Prüfer D, Tacke E, Schmitz J, Kuli B, Kaufmann A, Rohde W (1992) Ribosomal frameshifting in plants: a novel signal directs the -1 frameshift in the synthesis of the putative viral replicase of potato leafroll luteovirus. EMBO J 11: 1111–1117PubMedGoogle Scholar
  205. Qiu SG, Schoelz JE (1992) Three regions of cauliflower mosaic virus strain W260 are involved in systemic infection of solanaceous hosts. Virology 190: 773–782PubMedGoogle Scholar
  206. Quadt R, Kao CC, Browning KS, Hershberger RP, Ahlquist P (1993) Characterisation of a host protein associated with brome mosaic virus RNA-dependent RNA polymerase. Proc Natl Acad Sci USA 90: 1498–1502PubMedGoogle Scholar
  207. Quillet L, Guilley H, Jonard G, Richards K (1989) In vitro synthesis of biologically active beet necrotic yellow vein virus RNA. Virology 172: 293–301PubMedGoogle Scholar
  208. Rao ALN, Grantham GL (1996) Molecular studies on bromovirus capsid protein. II. Functional analysis of the amino-terminal arginine-rich motif and its role in encapsidation, movement, and pathology. Virology 226: 294–305PubMedGoogle Scholar
  209. Register JC, Nelson RS (1992) Early events in plant virus infection: relationships with genetically engineered protection and host gene resistance. Semin Virol 3: 441–451Google Scholar
  210. Reichel C, Mathur J, Eckes P, Langenkemper K, Koncz C, Schell J, Reiss B, Maas C (1996) Enhanced green fluorescence by the expression of an Aequorea victoria green fluorescent protein mutant in mono- and dicotyledonous plant cells. Proc Natl Acad Sci USA 93: 5888–5893PubMedGoogle Scholar
  211. Reid MS, Matthews REF (1966) On the origin of the mosaic induced by turnip yellow mosaic virus. Virology 28: 563–570PubMedGoogle Scholar
  212. Rhodes J, Thain JF, Wildon DC (1996) The pathway for systemic electrical signal conduction in the wounded tomato plant. Planta 200: 50–57Google Scholar
  213. Riesmeier JW, Himer B, Frommer WB (1993) Potato sucrose transporter expression in minor veins indicates a role in phloem loading. Plant Cell 5: 1591–1598PubMedGoogle Scholar
  214. Rigden JE, Dry IB, Mullineaux PM, Rezaian MA (1993) Mutagenesis of the virion-sense open reading frames of tomato leaf curl geminivirus. Virology 193: 1001–1005PubMedGoogle Scholar
  215. Roberts A, Santa Cruz S, Roberts IM, Prior DAM, Turgeon R, Oparka K (1997) Phloem- unloading in sink leaves of Nicotiana benthamiana is symplastic and regulated by class III veins: comparison of fluorescent solute with fluorescent virus. Plant cell (in press)Google Scholar
  216. Robinson-Beers K, Evert RF (1991a) Fine structure of plasmodesmata in mature leaves of surgarcane. Planta 184: 307–318Google Scholar
  217. Robinson-Beers K, Evert RF (1991b) Ultrastructure of and plasmodesmatal frequency in mature leaves of surgarcane. Planta 184: 291–306Google Scholar
  218. Rochon DM, Johnston JC, Rivière CJ (1991) Molecular analysis of the cucumber necrosis virus genome: Can J Plant Pathol 13: 142–154Google Scholar
  219. Roossinck MJ, Palukaitis P (1990) Rapid induction and severity of symptoms in zucchini squash (Cucurbita pepo) map to RNA 1 of cucumber mosaic virus. Mol Plant Microbe Interact 3: 188–192Google Scholar
  220. Russell SH, Evert RF (1985) Leaf vasculature in Zea mays L. Planta 164: 448–458Google Scholar
  221. Russin WA, Evert RF (1985) Studies on the leaf of Populus deltoides (Salicaceae): ultrastructure, plasmodesmatal frequency and solute concentrations. Am J Bot 72: 1232–1247Google Scholar
  222. Russin WA, Evert RF, Vanderveer PJ, Sharkey TD, Briggs SP (1996) Modification of a specific class of plasmodesmata and loss of sucrose export ability in the sucrose export defective 1 maize mutant. Plant Cell 8: 645–658PubMedGoogle Scholar
  223. Sacher R, Ahlquist P (1989) Effects of deletion in the N-terminal base arm of brome mosaic virus coat protein on RNA packaging and systemic infection. J Virol 63: 4545–4552PubMedGoogle Scholar
  224. Saito T, Yamanaka K, Okada Y (1990) Long-distance movement and viral assembly of tobacco mosaic virus mutants. Virology 176: 329–336PubMedGoogle Scholar
  225. Samuel G (1934) The movement of tobacco mosaic virus within the plant. Ann Appi Biol 21: 90–111Google Scholar
  226. Sanderfoot AA, Lazarowitz SG (1996) Getting it together in plant virus movement: cooperative interactions between bipartite geminivirus movement proteins. Trends Cell Biol 6: 353–358PubMedGoogle Scholar
  227. Sänger HL (1969) Functions of the two particles of tobacco rattle virus. J Virol 3: 304–312PubMedGoogle Scholar
  228. Sanger M, Passmore B, Falk BW, Bruening G, Ding B, Lucas WJ (1994) Symptom severity of beet western yellows virus strain ST9 is conferred by the ST9-associated RNA and is not associated with virus release from the phloem. Virology 200: 48–55PubMedGoogle Scholar
  229. Santa Cruz S, Chapman S, Roberts AG, Roberts IM, Prior DAM, Oparka KJ (1996) As-sembly and movement of a plant virus carrying a green fluorescent protein overcoat. Proc Natl Acad Sci USA 93: 6286–6290PubMedGoogle Scholar
  230. Schaad MC, Carrington JC (1996) Suppression of long-distance movement of tobacco etch virus in a nonsusceptible host. J Virol 70: 2556–2561PubMedGoogle Scholar
  231. Schmalstig JG, Geiger DR (1985) Phloem unloading in developing leaves of sugar beet. I. Evidence for pathway through the symplast. Plant Physiol 79: 237–241PubMedGoogle Scholar
  232. Schmalstig JG, Geiger DR (1987) Phloem unloading in developing leaves of sugar beet. II. Termination of phloem unloading. Plant Physiol 83: 49–52PubMedGoogle Scholar
  233. Schmitz I, Rao ALN (1996) Molecular studies on bromovirus capsid protein. I. Characterization of cell-to-cell movement-defective RNA3 variants of brome mosaic virus. Virology 226: 281–293PubMedGoogle Scholar
  234. Schmitz J (1995) Molekularbiologische Studien am vermuteten Transportprotein des potato leafroll virus (PLRV). Untersuchungen zur Lokalisation und alternativen Expression von prl7. PhD Thesis, University of CologneGoogle Scholar
  235. Schmitz K, Cuypers B, Moll M (1987) Pathway of assimilate transfer between mesophyll cells and minor veins in leaves of Cucumis melo L. Planta 171: 19–29Google Scholar
  236. Schneider IR, Worley JF (1959) Rapid entry of infectious particles of southern bean mosaic virus into living cells following transport of the particles in the water stream. Virology 8: 243–249PubMedGoogle Scholar
  237. Schneider WL, Green AE, Allison RF (1997) The carboxy-terminal two-thirds of the cow- pea chlorotic mottle bromovirus capsid protein is incapable of virion formation yet supports systemic movement. J Virol 71: 4862–4865PubMedGoogle Scholar
  238. Schobert C, Großmann P, Gottschalk M, Komor E, Pecsvaradi A, zur Nieden U (1995) Sieve-tube exudate from Ricinus communis L. seedlings contains ubiquitin and chaperones. Planta 196: 205–210Google Scholar
  239. Scholthof HB, Morris TJ, Jackson AO (1993) The capsid protein gene of tomato bushy stunt virus is dispensable for systemic movement and can be replaced for localized expression of foreign genes. Mol Plant Microbe Interact 6: 309–322Google Scholar
  240. Scholthof HB, Scholthof K-BG, Kikkert M, Jackson AO (1995) Tomato bushy stunt virus spread is regulated by two nested genes that function in cell-to-cell movement and host-dependent systemic invasion. Virology 213: 425–438PubMedGoogle Scholar
  241. Schulz A (1995) Plasmodesmal widening accompanies the short-term increase in symplasmic phloem unloading in pea root tips under osmotic stress. Protoplasma 188: 22–37Google Scholar
  242. Séron K, Haenni A-L (1996) Vascular movement of plant viruses. Mol Plant Microbe Interact 9: 435–442PubMedGoogle Scholar
  243. Shaw JG, Piaskitt KA, Wilson TMA (1986) Evidence that tobacco mosaic virus particles disassemble contranslationally in vivo. Virology 148: 326–336PubMedGoogle Scholar
  244. Shepardson S, Esau K, McCrum R (1980) Ultrastructure of potato leaf phloem infected with potato leafroll virus. Virology 105: 379–392PubMedGoogle Scholar
  245. Shintaku MH, Carter SA, Bao Y, Nelson RS (1996) Mapping nucleotides in the 126-kDa protein gene that control the differential symptoms induced by two strains of tobacco mosaic virus. Virology 221: 218–225PubMedGoogle Scholar
  246. Siegel A, Hari V, Kolacz K (1978) The effect of tobacco mosaic virus infection on host and virus-specific protein synthesis in protoplasts. Virology 85: 494–503PubMedGoogle Scholar
  247. Siegel A, Zaitlin M, Sehgal OP (1962) The isolation of defective tobacco moasic virus strains. Proc Natl Acad Sci USA 48: 1845–1851PubMedGoogle Scholar
  248. Simon AE, Li XH, Lew JE, Stange R, Zhang C, Polacco M, Carpenter CD (1992) Susceptibility and resistance of Arabidopsis thaliana to turnip crinkle virus. Mol Plant Microbe Interact 5: 496–503Google Scholar
  249. Sokolova M, Prüfer D, Tacke E, Rohde W (1997) The potato leafroll virus 17K movement protein is phosphorylated by a membrane-associated protein kinase from potato with biochemical features of protein kinase C. FEBS Lett 400: 201–205PubMedGoogle Scholar
  250. Solberg RA, Bald JG (1962) Virus invasion and multiplication during leaf histogenesis. Virology 17: 359–361PubMedGoogle Scholar
  251. Stadler R, Brandner J, Schulz A, Gahrtz M, Sauer N (1995) Phloem loading by the PmSUC2 sucrose carrier from Plantago major occurs into companion cells. Plant Cell 7: 1545–1554PubMedGoogle Scholar
  252. Suzuki M, Kuwata S, Kataoka J, Masuta C, Nitta N, Takanami Y (1991) Functional analysis of deletion mutants of cucumber mosaic virus RNA3 using an in vitro transcription system. Virology 183: 106–113PubMedGoogle Scholar
  253. Tacke E, Schmitz J, Priifer D, Rohde W (1993) Mutational analysis of the nucleic acid- binding 17 kDa phosphoprotein of potato leafroll luteovirus identifies an amphipathic a-helix as the domain for protein/protein interactions. Virology 197: 274–282PubMedGoogle Scholar
  254. Takamatsu N, Ishikawa M, Meshi T, Okada Y (1987) Expression of bacterial chloramphenicol acetyltransferase gene in tobacco plants mediated by TMV-RNA. EMBO J 6: 307–311PubMedGoogle Scholar
  255. Taliansky ME, Garcia-Arenal F (1995) Role of cucumovirus capsid protein in long distance movement within the infected plant. J Virol 69: 916–922PubMedGoogle Scholar
  256. Taliansky ME, Malyshenko SI, Kaplan IB, Kondakova OA, Atabekov JG (1992) Production of the tobacco mosaic virus (TMV) transport protein in transgenic plants is essential but insufficient for complementing foreign virus transport: a need for the full- length TMV genome or some other TMV-encoded product. J Gen Virol 73: 471–474PubMedGoogle Scholar
  257. Tóth KF, Wang Q, Sjfilund RD (1994) Monoclonal antibodies against phloem P-protein from plant tissue cultures. I. Microscopy and biochemical analysis. Am J Bot 81: 1370–1377Google Scholar
  258. Traynor P, Young BM, Ahlquist P (1991) Deletion analysis of brome mosaic virus 2a protein: effects on RNA replication and systemic spread. J Virol 65: 2807–2815PubMedGoogle Scholar
  259. Truernit E, Sauer N (1995) The promoter of Arabidopsis thaliana SUC2 sucrose H+ symporter gene directs expression of p-glucuronidase to the phloem: evidence for phloem loading and unloading by SUC2. Planta 196: 564–570PubMedGoogle Scholar
  260. Tucker EB (1993) Azide treatment enhances cell-to-cell diffusion in staminal hairs of Setcreasea purpurea. Protoplasma 174: 45–49Google Scholar
  261. Turgeon R (1984) Termination of nutrient import and development of vein loading capacity in albino tobacco leaves. Planta 76: 45–48Google Scholar
  262. Turgeon R (1986) The import-export transition in dicotyledonous leaves. In: Cronshaw J, Lucas WJ, Giaquinta RT (eds) Phloem transport. Liss, New York, pp 285–291Google Scholar
  263. Turgeon R (1987) Phloem unloading in tobacco sink leaves: insensitivity to anoxia indicates a symplastic pathway. Planta 171: 73–81Google Scholar
  264. Turgeon R (1989) The sink-source transition in leaves. Annu Rev Plant Physiol Plant Mol Biol 40: 119–138Google Scholar
  265. Turgeon R (1996) Phloem loading and plasmodesmata. Trends Plant Sci 1: 418–423Google Scholar
  266. Turgeon R, Hepler PK (1989) Symplastic continuity between mesophyll and companion cells in minor veins of mature Cucurbita pepo L. leaves. Planta 179: 24–31Google Scholar
  267. Turgeon R, Webb JA (1973) Leaf development and phloem transport in Cucurbita pepo; transition from import to export. Planta 113: 179–191Google Scholar
  268. Turgeon R, Wimmers LE (1988) Different patterns of vein loading of exogenous [14C]-sucrose in leaves of Pisum sativum and Coleus blumei. Plant Physiol 87: 179–182PubMedGoogle Scholar
  269. Turgeon R, Webb JA, Evert RF (1975) Ultrastructure of minor veins of Cucurbita pepo leaves. Protoplasma 83: 217–232Google Scholar
  270. Turgeon R, Beebe DU, Gowan E (1993) The intermediary cell: minor-vein anatomy and raffinose oligosaccharide synthesis in the Scrophulariaceae. Planta 191: 446–456Google Scholar
  271. Urban LA, Ramsdell DC, Klomparens KL, Lynch T, Hancock JF (1989) Detection of blueberry shoestring virus in xylem and phloem tissues of highbush blueberry. Phytopathology 79: 488–493Google Scholar
  272. Vaewhongs AA, Lommel SA (1995) Virion formation is required for the long-distance movement of red clover necrotic mosaic virus in movement protein transgenic plants. Virology 212: 607–613PubMedGoogle Scholar
  273. Valkonen JPT, Somersalo S (1996) Patterns and barriers of cell-to-cell movement and lack of systemic spread of tobacco etch potyvirus (TEV-GUS) in Solatium brevidens. Plant Sci 113: 221–228Google Scholar
  274. Van Bel AJE (1990) Xylem-phloem exchange via the rays: the undervalued route of transport. J Exp Bot 41: 631–644Google Scholar
  275. Van Bel AJE (1993) Strategies of phloem loading. Annu Rev Plant Physiol Plant Mol Biol 44: 253–281Google Scholar
  276. Van Bel AJE (1996a) Carbohydrate processing in the mesophyll trajectory in symplasmic and apoplasmic phloem loading. Prog Bot 57: 140–167Google Scholar
  277. Van Bel AJE (1996b) Interaction between sieve element and companion cell and the consequences for photoassimilate distribution. Two structural hardware frames with associated software packages in dicotyledons? J Exp Bot 47: 1129–1140PubMedGoogle Scholar
  278. Van Bel AJE, Gamalei YV (1992) Ecophysiology of phloem loading in source leaves. Plant Cell Environ 15: 265–270Google Scholar
  279. Van Bel AJE, Kempers R (1991) Symplastic isolation of the sieve element/companion cell complex in the phloem of Ricirtus communis and Salix alba stems. Planta 183: 69–76Google Scholar
  280. Van Bel AJE, Kempers R (1997) The pore/plasmodesm unit; key element in the interplay between sieve element and companion cell. Prog Bot 58: 278–291Google Scholar
  281. Van Bel AJE, Oparka KJ (1995) On the validity of plasmodesmograms. Bot Acta 108: 174–182Google Scholar
  282. Van Bel AJE, Van Rijen HVM (1994) Microelectrode-recorded development of the symplasmic autonomy of the sieve element/companion cell complex in the stem phloem of Lupinus luteus L. Planta 192: 165–175Google Scholar
  283. Van Bel AJE, van Kesteren WJP, Papenhuijzen C (1988) Ultrastructural indications for coexistence of symplastic and apoplastic phloem loading in Commelina benghalesis leaves. Planta 176: 159–172Google Scholar
  284. Van Bel AJE, Ammerlaan A, van Dijk AA (1994) A three-step screening procedure to identify the mode of phloem loading in intact leaves. Evidence for symplasmic and apoplasmic phloem loading associated with the type of companion cell. Planta 192: 31–39Google Scholar
  285. Van Bel AJE, Gamalei YV, Ammerlaan A, Bik LPM (1992) Dissimilar phloem loading in leaves with symplasmic and apoplasmic minor-vein configurations. Planta 186: 518–525Google Scholar
  286. Van den Heuvel JFJM, De Blank CM, Peters D, Van Lent JWM (1995) Localization of potato leafroll virus in leaves of secondarily-infected potato plants. Eur J Plant Pathol 101: 567–571Google Scholar
  287. Van der Kuyl AC, Neeleman L, Bol JF (1991) Complementation and recombination between alfalfa mosaic virus RNA3 mutants in tobacco plants. Virology 183: 731–738PubMedGoogle Scholar
  288. Van der Schoot C, Van Bel AJE (1989) Glass microelectrode measurements of sieve tube membrane potentials in internode discs and petiole strips of tomato (Solarium ly-copersicum L.). Protoplasma 149: 144–154Google Scholar
  289. Van der Schoot C, Van Bel AJE (1990) Mapping membrane potential differences and dye-coupling in internodal tissues of tomato (Solatium lycopersicum L.) Planta 182: 9–21Google Scholar
  290. Van Lent JWM, Verduin BJM (1987) Detection of viral antigen in semi-thin sections of plant tissue by immunogold-silver staining and light microscopy. Neth J Pathol 93: 261–272Google Scholar
  291. Verduin BJM (1992) Early interactions between viruses and plants. Semin Virol 3: 423–431Google Scholar
  292. Volk GM, Turgeon R, Beebe DU (1996) Secondary plasmodesmata formation in the minor-vein phloem of Cucumis melo L. and Cucurbita pepo L. Planta 199: 425–432Google Scholar
  293. Waigmann E, Lucas WJ, Citovsky V, Zambryski P (1994) Direct functional assay for tobacco mosaic virus cell-to-cell movement protein and identification of a domain involved in increasing plasmodesmal permeability. Proc Natl Acad Sci USA 91: 1433–1437PubMedGoogle Scholar
  294. Walsh MA (1974) Late formed metaphloem sieve elements in Zea mays L. Planta 121: 17–25Google Scholar
  295. Wang HL, Gilbertson RL, Lucas WJ (1996) Spatial and temporal distribution of bean dwarf mosaic geminivirus in Phaseolus vulgaris and Nicotiana benthamiana. Phytopathology 86: 1204–1214Google Scholar
  296. Wang Q, Monroe J, Sjolund RD (1995) Identification and characterization of a phloem-specific p-amylase. Plant Physiol 109: 743–750PubMedGoogle Scholar
  297. Warmbrodt R (1985a) Studies on the root of Zea mays L. - Structure of the adventitious roots with respect to phloem unloading. Bot Gaz 146: 169–180Google Scholar
  298. Warmbrodt R (1985b) Studies on the root of Hordeum vulgare L. - ultrastructure of the seminal root with special reference to the phloem. Am J Bot 72: 414–432Google Scholar
  299. Warmbrodt R (1986) Structural aspects of the primary tissues of the Cucurbita pepo L. roots with special reference to the phloem. New Phytol 102: 175–192Google Scholar
  300. Warmbrodt R, van der Woude WJ (1990) Leaf of Spinacia oleracea (spinach): ultrastructure, and plasmodesmatal distribution and frequency, in relation to sieve-tube loading. Am J Bot 77: 1361–1377Google Scholar
  301. Watanabe Y, Emori Y, Ooshika I, Meshi T, Ohno T, Okada Y (1984) Synthesis of TMV- specific RNAs and proteins at the early stage of infection in tobacco protoplasts: transient expression of the 30K protein and its mRNA. Virology 133: 18–24PubMedGoogle Scholar
  302. Watanabe Y, Morita N, Nishiguchi M, Okada Y (1987) Attenuated strains of tobacco mosaic virus reduced synthesis of a viral protein with a cell-to-cell movement function. J Mol Biol 194: 699–704PubMedGoogle Scholar
  303. Wegner LH (1996) The role of ion channels in salt transport between the xylem and adjacent cells. PhD Thesis, University of AmsterdamGoogle Scholar
  304. Weiland J J, Edwards MC (1994) Evidence that the aa gene of barley stripe mosaic virus encodes determinants of pathogenicity to oat (Avena sativa). Virology 201: 116–126PubMedGoogle Scholar
  305. Weiland JJ, Edwards MC (1996) A single nucleotide substitution in the aa gene confers oat pathogenicity to barley stripe mosaic virus strain ND18. Mol Plant Microbe Interact 9: 62–67PubMedGoogle Scholar
  306. Welbaum GE, Meinzer FC, Grayson RL, Thornham KT (1992) Evidence for and consequences of a barrier to solute diffusion between the apoplast and vascular bundles in sugarcane stalk tissue. Aust J Plant Physiol 19: 611–623Google Scholar
  307. Wellink J, van Kammen AB (1989) Cell-to-cell transport of cowpea mosaic virus requires both the 58K/48K proteins and the capsid proteins. J Gen Virol 70: 2279–2286Google Scholar
  308. White RF, Sugars JM (1996) The systemic infection by tobacco mosaic virus of tobacco plants containing the N gene at temperatures below 28°C. J Phytopathol 144: 139–142Google Scholar
  309. Whitham S, Dinesh-Kumar SP, Choi D, Hehl R, Corr C, Baker B (1994) The product of the tobacco mosaic virus resistance gene N: similarity to toll and the interleukin-1 receptor. Cell 78: 1101–1115PubMedGoogle Scholar
  310. Williams ML, Farrar JF, Pollock CJ (1989) Cell specialization within the parenchymatous bundle sheath of barley. Plant Cell Environ 12: 909–918Google Scholar
  311. Wilson CR, Jones RAC (1992) Resistance to phloem transport of potato leafroll virus in potato plants. J Gen Virol 73: 3219–3224PubMedGoogle Scholar
  312. Wilson TMA, Shaw JG (1987) Cotranslational disassembly of filamentous plant virus nucleocapsids in vitro and in vivo. In: Brinton MA, Rueckert RR (eds) Positive strand RNA viruses. Liss, New York, pp 159–181Google Scholar
  313. Wilson TMA, Plaskitt KA, Watts JW, Osbourn JK, Watkins PAC (1990) Signals and structures involved in early interactions between plants and viruses or pseudoviruses. In: Fraser RSS (ed) Recognition and response in plant-virus interactions. Springer, Berlin Heidelberg New York, pp 123–145Google Scholar
  314. Wimmers LE, Turgeon R (1991) Transfer cells and solute uptake in minor veins of Pisum sativum leaves. Planta 186: 2–12Google Scholar
  315. Wintermantel WM, Anderson EJ, Schoelz JE (1993) Identification of domains within gene VI of cauliflower mosaic virus that influence systemic infection of Nicotiana bigelovii in a light-dependent manner. Virology 196: 789–798PubMedGoogle Scholar
  316. Wintermantel WM, Banerjee N, Oliver JC, Paolillo DJ, Zaitlin M (1997) Cucumber mosaic virus is restricted from entering minor veins in transgenic tobacco exhibiting replicase-mediated resistance. Virology 231: 248–257PubMedGoogle Scholar
  317. Wisniewski HM, Ashworth E, Schaffer K (1987) The use of lanthanum to characterize cell wall permeability in relation to deep supercooling and extracellular freezing in woody plants. I. Intergeneric comparisons between Prunus, Cornus, and Salix. Protoplasma 139: 105–116Google Scholar
  318. Wisniewski LA, Powell PA, Nelson RS, Beachy RN (1990) Local and systemic spread of tobacco mosaic virus in transgenic tobacco. Plant Cell 2: 559–567PubMedGoogle Scholar
  319. Wood RM, Patrick JW, Offler CE (1997) The cellular pathway of short-distance transfer of photosynthate and potassium in the elongating stem of Phaseolus vulgaris L. A structural assessment. Ann Bot 79: 89–100Google Scholar
  320. Wright KM, Oparka KJ (1997) Metabolic inhibitors induce symplastic movement of solutes from the pathway phloem of Arabidopsis roots. J Exp Bot 48 (in press)Google Scholar
  321. Wu X, Shaw J (1996) Bidirectional uncoating of the genomic RNA of a helical virus. Proc Natl Acad Sci USA 93: 2981–2984PubMedGoogle Scholar
  322. Wu X, Xu Z, Shaw JG (1994) Uncoating of tobacco mosaic virus RNA in protoplasts. Virology 200: 256–262PubMedGoogle Scholar
  323. Wyatt SD, Kuhn CW (1980) Derivation of a new strain of cowpea chlorotic mottle virus from resistant cowpeas. J Gen Virol 49: 289–296Google Scholar
  324. Xiong Z, Kim KH, Giesman-Cookmeyer D, Lommel SA (1993) The roles of the red clover necrotic mosaic virus capsid and cell-to-cell movement proteins in systemic infection. Virology 192: 27–32PubMedGoogle Scholar
  325. Yang F, Moss LG, Phillips GN (1996) The molecular structure of green fluorescent protein. Nat Biotechnol 14: 1246–1251PubMedGoogle Scholar
  326. Zech H (1952) Untersuchungen über den Infektionsvorgang und die Wanderung des Tabakmosaikvirus im Pflanzenkörper. Planta 40: 461–514Google Scholar
  327. Ziegler-Graff V, Brault V, Mutterer JD, Simonis M-T, Herrbach E, Guilley H, Richards KE, Jonard G (1996) The coat protein of beet western yellows luteovirus is essential for systemic infection but the viral gene products P29 and P19 are dispensable for systemic infection and aphid transmission. Mol Plant Microbe Interact 9: 501–510Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Richard S. Nelson
    • 1
  • Aart J. E. van Bel
    • 2
  1. 1.Plant Biology DivisionThe Samuel Roberts Noble FoundationArdmoreUSA
  2. 2.Institut für Allgemeine Botanik und PflanzenphysiologieJustus-Liebig Universität GiessenGiessenGermany

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