Somatic Embryogenesis in Asparagus

  • A. Levi
  • K. C. Sink
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 31)


The genus Asparagus belongs to the family Liliaceae, and is comprised of about 150–300 herbaceous and woody perennial species spread throughout the temperate and tropical regions of the world (Lawrence 1982). Among these species Asparagus officinalis L. is the most well-known edible, and is considered one of the oldest garden vegetable plants (Luzny 1979). It is an European-Siberian continental plant which belongs to the northeast Mediterranean vegetation gene center (Luzny 1979), where it is well adapted to sandy soils. Asparagus is widely distributed and grown as a high-value crop in different parts of the world.


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  1. Chin CK (1982) Promotion of shoot and root formation in asparagus in vitro by ancymidol. HortScience 17: 590–591Google Scholar
  2. Doré C (1990) Asparagus anther culture and field trials of dihaploids and F1 hybrids. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 12. Haploids in crop improvement I. Springer, Berlin Heidelberg, New York, pp 322–345Google Scholar
  3. Ellison JH (1986) Asparagus breeding. In: Bassett MJ (ed) Breeding vegetable crops. Avi, Westport, pp 523–569Google Scholar
  4. Hasegawa PM, Murashige T, Takatori FH (1973) Propagation of asparagus through shoot apex culture. II. Light and temperature requirements, transplantability of plants, and cytohistological characteristics. J Am Soc Hortic Sci 98: 143–148Google Scholar
  5. Lawrence GHM (1982) Taxonomy of vascular plants. Macmillan, New York, 823 ppGoogle Scholar
  6. Levi A, Sink KC (1990) Differential effects of sucrose, glucose and fructose during somatic embryogenesis in asparagus. J Plant Physiol 137: 184–189Google Scholar
  7. Levi A, Sink KC (1991a) Somatic embryogenesis in asparagus: the role of expiants and growth regulators. Plant Cell Rep 10: 71–75CrossRefGoogle Scholar
  8. Levi A, Sink KC (1991b) Histology and morphology of asparagus somatic embryos. HortScience 10: 1322–1324Google Scholar
  9. Levi A, Sink KC (1992) Asparagus somatic embryos: Production in suspension culture and conversion to plantlets on solidified medium as influenced by carbohydrate regime. Plant Cell Tissue Organ Cult 31: 115–122CrossRefGoogle Scholar
  10. Linsamier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100–127CrossRefGoogle Scholar
  11. Loptien H (1979) Identification of the sex chromosome pair in asparagus (Asparagus officinalis L.). Z Pflanzenzücht 82: 162–173Google Scholar
  12. Luzny J (1979) The history of asparagus as a vegetable, the tradition of its growing in Czechoslovakia (CSSR) and prospect of its further propagation and breeding. In: Reuther G (ed) Proc 5th Int Asparagus Symp, Eucarpia Section Vegetables. Geisenheim Forschungsanstalt, GermanyGoogle Scholar
  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  14. Peirce LC (1987) Vegetables: characteristics, production and marketing. John Wiley, New YorkGoogle Scholar
  15. Reuther G (1977) Adventitious organ formation and somatic embryogenesis in callus of asparagus and iris and its possible application. Acta Hortic 78: 217–224Google Scholar
  16. Reuther G (1984) Asparagus. In: Sharp WR, Evans DA, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 2. Macmillan, New York, pp 211–242Google Scholar
  17. Steward FC, Mapes MO (1971) Morphogenesis and plant propagation in aseptic cultures of asparagus. Bot Gaz 132: 70–79CrossRefGoogle Scholar
  18. Volokita M, Levi A, Sink KC (1987) A revised protocol for micropropogation of asparagus. Asparagus Res Newslett 4: 8–17Google Scholar
  19. Wilmar C, Hellendoorn M (1968) Growth and morphogenesis of Asparagus cells cultured in vitro. Nature 217: 369–370CrossRefGoogle Scholar
  20. Wricke G (1979) Breeding research in Asparagus officinalis: introductory remarks. In: Reuther G (ed) Proc 5th Int Asparagus Symp, Ecarpia Section Vegetables. Geisenheim Forschungsanstalt, Germany, pp 1–7Google Scholar
  21. Yang HJ (1977) Tissue culture technique developed for asparagus propagation. HortScience 12: 140–141Google Scholar
  22. Yang H J, Clore WJ (1973) Aerial crown in Asparagus officinalis L. HoriScience 8: 33Google Scholar
  23. Yang J, Clore WJ (1975) In vitro reproductiveness of asparagus stem segments with branch-shoots at a node. HortScience 10: 411–412Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • A. Levi
    • 1
  • K. C. Sink
    • 2
  1. 1.Fruit Laboratory, BARC-WestUSDA-ARS, PSIBeltsvilleUSA
  2. 2.Department of HorticultureMichigan State UniversityEast LansingUSA

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