Abstract
Since the phrase “fine-structure preservation” is central to the theme of this book, it is important to define precisely what it means. It is relatively easy to give a theoretical definition: — cell structure should be preserved exactly as it was in the living state and should be visualized at the resolution limit of the electron microscope.
Keywords
Electron Microscopy Section Specimen Preparation Technique Zellforsch Mikrosk Anat Knife Mark Normal Transmission Electron Microscopy
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Preview
Unable to display preview. Download preview PDF.
References
- Balch WE, Wagner KR, Keller DS (1987) Reconstitution of transport of vesicular stomatitis virus G protein from the endoplasmic reticulum to the Golgi complex using a cell-free system. J Cell Biol 104:749–760PubMedCrossRefGoogle Scholar
- Bernhard W, Haguenau F, Gautier A, Oberling Ch (1952) La structure submicroscopique des elements basophiles cytoplasmiques dans le foie le pancréas et les glandes salivaires. Z Zellforsch Mikrosk Anat 37:281–289CrossRefGoogle Scholar
- Carlemalm E, Kellenberger E (1982) The reproducible observation of unstained embedded cellular material in thin sections: visualisation of an integral membrane protein by a new mode of imaging for STEM. EMBO J 1:63–67PubMedGoogle Scholar
- Chang J J, Mowall AW, Freeman R, Walter CA, Dubochet J (1983) Freezing sectioning and observing artefacts of frozen hydrated sections for electron microscopy. J Microsc 132:109–123CrossRefGoogle Scholar
- Dalton AI, Felix MD (1952) Studies of the Golgi substance of the epithelial cells of the epidymis and duodenum of the mouse. Am J Anat 92:277–305CrossRefGoogle Scholar
- Dubochet J, Mowell AW, Menge B, Schmid EN, Lickfeld KG (1983b) Electron miscroscopy of frozen-hydrated bacteria. J Bact 155:381–390PubMedGoogle Scholar
- Ebersold HR, Cordier IL, Lüthy P (1981) Bacterial mesosomes: method dependent artefacts. Arch Microbiol 130:19–22PubMedCrossRefGoogle Scholar
- Elias H (1972) Identification of structure by the common-sense approach. J Microsc 95:59–68PubMedCrossRefGoogle Scholar
- Fooke-Achterrath M, Lickfeld KG, Reutsch VM Jr, Aebi U, Tschöpe U, Menge B (1974) Close-to-life preservation of Staphylococcus aureus mesosomes for transmission electron microscopy. J Ultrastruct Res 49:270–285PubMedCrossRefGoogle Scholar
- Griffiths G, Hoflack B, Simons K, Mellman I, Kornfeld S (1988) The mannose 6-phosphate receptor and the biogenesis of lysosomes. Cell 52:329–341PubMedCrossRefGoogle Scholar
- Griffiths G, Matteoni R, Back R, Hoflack B (1990) Characterization of the cation-independent mannose 6-phosphate receptor-enriched prelysosomal compartment in NRK cells. J Cell Sci 95:441–461PubMedGoogle Scholar
- Hayat MA (1981) Fixation for electron microscopy. Academic Press, New YorkGoogle Scholar
- Kellenberger E, Carlemalm E, Villiger W (1986) Physics of specimen preparation and observation of specimens that involve cryoprocedures. In: Science of biological specimen preparation. SEM Inc, AMF O’Hare (Chicago) USA, pp 1–20Google Scholar
- Ledbetter M, Porter K (1963) A “microtubule” in plant cell fine structures. J Cell Biol 19:239–250PubMedCrossRefGoogle Scholar
- McDowall AW, Chang JJ, Freeman R, Lepault L, Walter CA, Dubochet J (1983) Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samples. J Microsc 131:1–9PubMedCrossRefGoogle Scholar
- McDowall AW, Gruenberg J, Römisch K, Griffiths G (1989) The structure of organelles of the endocytic pathway in hydrated cryosections of cultured cells. Eur J Cell Biol 49:281–294PubMedGoogle Scholar
- Nass MMK, Buck CA (1963a) Intramitochondrial fibres with DNA chartacteristics. I. Fixation and electron staining reactions. J Cell Bio 19:593–612Google Scholar
- Nass S, Nass MMK (1963b) Intramitochondrial fibres with DNA characteristics. II. Enzymatic and other hydrolytic treatments. J Cell Bio 19:613–621Google Scholar
- Palade GE (1975) Intracellular aspects of the process of protein synthesis. Science 189:347–357PubMedCrossRefGoogle Scholar
- Palade GE, Claude A (1949) The nature of the Golgi apparatus. Parallelism between Golgi apparatus and intracellular myelin figures. J Morphol 85:35–112Google Scholar
- Palade GE, Porter KR (1954) Studies on the endoplasmic reticulum. I. Its identification in cells in situ. J Exp Med 100:641–650Google Scholar
- Parsons DF (1972) Status of X-ray diffraction of membranes. Ann NY Acad Sci 145:321–328CrossRefGoogle Scholar
- Porter KA (1953) Observations on a submicroscopic basophilic component of the cytoplasm. J Exp Med 97:727–735PubMedCrossRefGoogle Scholar
- Porter KR, Tucker JB (1981) The ground substance of the living cell. Sci Am 244:40–46CrossRefGoogle Scholar
- Rash JE, Johnson TJA, Hudson CS, Giddings FD, Graham WF, Eldefrawi ME (1982) Labelled-replica techniques: post-shadow labelling of intramembrane particles in freeze-fracture replicas. J Microsc 128:121–138PubMedCrossRefGoogle Scholar
- Roth TF, Porter KR (1964) Yolk protein uptake in the oocyte of the mosquito Aedes aegypti. J Cell Biol 27:313–320CrossRefGoogle Scholar
- Sabatini DD, Bensch K, Barrnett RJ (1963) Aldehyde fixation for morphological and enzyme histochemical studies with the electron microscope. J Cell Biol 17:19–58PubMedCrossRefGoogle Scholar
- Simons K, Virta H (1987) Perforated MDK cells support intracellular transport. EMBO J 6:2241–2247PubMedGoogle Scholar
- Sjöstrand F (1953) Systems of double membranes in the cytoplasm of certain tissue cells. Nature 171:31–35CrossRefGoogle Scholar
- Slauterbach D (1963) Cytoplasmic microtubules 1. Hydra. J Cell Biol 18:367–388CrossRefGoogle Scholar
- Weibel ER (1979) Stereological methods. Practical methods for biological morphometry, vol. I. Academic Press, New YorkGoogle Scholar
- Wolosewick JJ, Porter KR (1979) Microtrabecular lattice of the cytoplasmic ground substance-artefact or reality. J Cell Biol 82:114–139PubMedCrossRefGoogle Scholar
Copyright information
© Springer-Verlag Berlin Heidelberg 1993