Epstein-Barr Virus Based Expression Vectors
Eukaryotic viral vectors have played an important role in the expression and analysis of many eukaryotic genes. The field is growing at a rapid pace and is by no means mature (for contrast see the reports from the first and second Banbury Conferences on Eukaryotic Viral Vectors, Gluzman 1982; Gluzman and Hughes 1988). Although in theory it should be possible to develop a eukaryotic vector from any virus, practical considerations have led to concentration on a few well-characterized viruses, e.g., papovaviruses, adenoviruses, herpesviruses, and retroviruses. As the understanding of the underlying biology of the virus’ life cycle has matured, so too has the sophistication of specific virus based vectors. Frequently the development of virus vectors has been instrumental in dissecting the functional elements of the parent virus. Present-day eukaryotic vectors are a collection of eukaryotic elements from many different cellular and viral sources. Unfortunately, there are no simple rules for constructing the best eukaryotic vector, hence much of this field has developed from empirical trials. Furthermore, no single vector presently exists that provides a solution for all situations that require a eukaryotic vector.
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- Cleary M, Dorfman RF, Sklar J (1986) Failure in immunological control of the virus: post-transplant lymphoma. In: Epstein MA, Achong BG (eds) The Epstein-Barr virus: recent advances. Wiley, New YorkGoogle Scholar
- Dillner J, Kallin B, Alexander H, Ernberg I, Uno M, Ono Y, Klein G, Lerner RA (1986) An Epstein-Barr virus (EBV)-determined nuclear antigen (EBNA5) partly encoded by the transformation-associated Bam WYH region of EBV DNA: preferential expression in lymphoblastoid cell lines. Proc Natl Acad Sci USA 83: 6641–6645PubMedCrossRefGoogle Scholar
- Fischer DK, Robert MF, Shedd D, Summers WP, Robinson JE, Wolak J, Stefano JE, Miller G (1984) Identification of Epstein-Barr nuclear antigen polypeptide in mouse and monkey cells after gene transfer with a cloned 2.9-kilobase-pair subfragment of the genome. Proc Natl Acad Sci USA 81:43–47PubMedCrossRefGoogle Scholar
- Gluzman Y (ed) (1982) Eurkaryotic viral vectors. Cold Spring Harbor Laboratories, New YorkGoogle Scholar
- Gluzman Y, Hughes SH (eds) (1988) Eukaryotic viral vectors. Cold Spring Harbor Laboratories, New YorkGoogle Scholar
- Heller R, Song K, Villaret D, Margolskee RF, Dunne J, Hayakawa H, Ringold GM (1990) Amplified expression of tumor necrosis factor receptor in cells transfected with Epstein-Barr virus shuttle vector cDNA libraries. J Biol Chem 264: 5708–5717Google Scholar
- Henle W, Henle G (1972) The relation of the Epstein-Barr virus to Burkitt’s lymphoma. In: Biggs RM, de-The G, Payne LN (eds) Oncogenesis and herpesviruses. IARC, LyonGoogle Scholar
- Jenson HB, Farrell PJ, Miller G (987) Sequences of the Epstein-Barr virus (EBV) large internal repeat form the center of a 16-kilobase-pair palindrome of EBV (P3HR-1) heterogeneous DNA. J Virol 61: 1495–1506Google Scholar
- Miller G (1985) Epstein-Barr virus. In: Fields BN (ed) Virology. Raven, New York, pp 563–589Google Scholar
- Strand BC, Schuster TC, Hopkins RF, Neubauer RH, Rabin H (1981) Identification of an Epstein-Barr virus nuclear antigen by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis. J Virol 38: 996–1004Google Scholar
- Sugden B (1984) Expression of virus-associated functions in cells transformed in vitro by Epstein-Barr virus: Epstein-Barr virus cell surface antigen and virus-release from transformed cells. In: Purtillo DT (ed) Immune deficiency and cancer, Epstein-Barr virus and lymphoproliferative malignancies. Plenum, New York, p 165Google Scholar